Flow & Image Cytometry Shared Resource

流动

• 批准号: 10398049
• 负责人: Kai Fu
• 金额: $ 21.33万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-16 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10398049
• 关键词: 1-Phosphatidylinositol 3-Kinase; Address; Advisory Committees; Agonist; Area; Award; Biological Assay; Buffaloes; CD80 gene; CD86 gene; CXCL12 gene; CXCR4 gene; Cancer Center Support Grant; Categories; Cells; Clinical; Collaborations; Confocal Microscopy; Consult; Consultations; Custom; Cytometry; Data Analyses; Detection; Development; Devices; Environment; Equipment; Experimental Designs; Fees; Flow Cytometry; Funding; Future; Gene Expression Profiling; Genomics Shared Resource; Goals; Guidelines; Human Resources; IFN consensus sequence binding protein; Image; Image Cytometry; Individual; Infrastructure; Institutes; Interleukin-6; L-Selectin; Leadership; Liver; Methodology; Microscope; Mission; Monitor; Multiple Myeloma; Myeloid-derived suppressor cells; NF-kappa B; NOTCH1 gene; Nature; Peer Review; Phenotype; Preparation; RNA; Radiation Protection; Reaction; Recommendation; Regulation; Reporting; Research; Research Personnel; Residual Neoplasm; Resolution; Resource Sharing; Resources; Role; Sampling; Secure; Services; Signal Transduction; Sorting - Cell Movement; Source; Specialist; Stains; Strategic Planning; Surveys; T-Cell Activation; T-Lymphocyte; TLR5 gene; Techniques; Technology; Testing; Transplant Recipients; United States National Institutes of Health; adaptive immunity; analytical tool; anti-cancer; anticancer research; antigen-specific T cells; base; cost effective; cost effectiveness; data acquisition; exosome; experimental study; graduate student; high dimensionality; imaging system; indexing; instrument; instrumentation; live cell imaging; lymph nodes; member; method development; nanoparticle; new technology; novel; oncolytic virotherapy; programs; quality assurance; remote control; tumor; user-friendly

PROJECT SUMMARY / ABSTRACT The mission of the Flow and Image Cytometry Shared Resource (FICSR) is to provide contemporary advanced flow cytometric analysis and sorting services, as well as imaging services at cellular and subcellular levels of resolution in a cost-effective, user-friendly and scientifically rigorous environment. A major strategic goal of the resource is to provide custom methods development services in a variety of labor-intensive, highly-specialized techniques. Development of methodology is often empirical in nature and cannot be easily obtained from outside sources. During the reporting period, FICSR has acquired new instrumentation, increased capacity, established new services, expanded educational programs, and engaged the Shared Resource Advisory Committee to develop a strategic plan that will enable us to continue to meet the rapidly evolving research needs of CCSG members. FICSR served a total of 134 Roswell users, of which 111 (93%) were CCSG members. There are three types of research investigators who use the FICSR. Level 1 users are those who are proficient in the techniques and know the instruments’ capabilities. For these investigators, the majority of our user base, the resource provides oversight and consultation. Level 2 users depend exclusively on the expertise of the resource staff to provide sample preparation, data acquisition, data analysis and its interpretation. Level 3 users include investigators who are new to flow and/or image cytometry or who are just beginning to consider new applications of this technology to address their research questions. Here the resource staff may perform pilot experiments to determine what would be a useful approach. The Specific Aims of the FICSR are: 1) To provide Roswell Park CCSG members access to cost-effective flow and image cytometry technology which is maintained according to a quality assurance program that follows regulatory agency guidelines and which meets the functional demands of their research programs; 2) To educate Roswell Park CCSG members on new developments in flow and image cytometry technology, their application and implementation pertaining to their specific research areas; 3) To enhance inter- and intra-programmatic collaborations by providing an educational and user-friendly environment in which FICSR personnel assist users with experimental design, execution, and analysis of their experiments commensurate with the user’s level of proficiency in the field. The resource offers services not provided by any other facility in the Buffalo region and the fees are among the lowest in the nation. The main objective of the FICSR is to stay on pace or ideally ahead of the cytometry needs of the CCSG user base. Thus, the resource leadership will continue close interactions with CCSG members to determine their needs and will also monitor the emergence of new technologies and analytic tools.

项目总结/摘要 流式细胞仪和图像细胞仪共享资源（FICSR）的使命是提供当代先进的 流式细胞仪分析和分选服务，以及细胞和亚细胞水平的成像服务， 在一个具有成本效益、用户友好和科学严谨的环境中解决问题。一个主要的战略目标， 资源是提供定制方法开发服务的各种劳动密集型，高度专业化 技术.方法的发展往往是经验性的，不能轻易地从 外部来源。在本报告所述期间，FICSR购置了新的仪器，提高了生产能力， 建立了新的服务，扩大了教育计划，并参与了共享资源咨询 委员会制定一项战略计划，使我们能够继续满足迅速发展的研究 CCSG成员的需求。FICSR共为134名Roswell用户提供服务，其中111名（93%）为CCSG 成员有三种类型的研究调查者使用FICSR。1级用户是指 熟练掌握技术并了解仪器的性能。对于这些研究人员来说，大多数 我们的用户群、资源提供监督和咨询。2级用户完全依赖于 资源工作人员的专业知识，提供样品制备，数据采集，数据分析及其 解释。3级用户包括流式细胞术和/或图像细胞术新手或刚刚接触流式细胞术的研究者 开始考虑这项技术的新应用，以解决他们的研究问题。这里 资源工作人员可以进行试点实验，以确定什么是有用的方法。具体目标 FICSR的目标是：1）为罗斯威尔公园CCSG成员提供具有成本效益的流程和形象 根据质量保证计划维护的流式细胞术技术， 机构的指导方针，并满足其研究计划的功能需求; 2）教育罗斯韦尔 Park CCSG成员介绍流式细胞术和图像细胞术技术的新发展， 3）加强方案间和方案内的合作， 通过提供一个教育和用户友好的环境，FICSR人员协助合作 用户的实验设计，执行和分析他们的实验与用户的 在该领域的熟练程度。该资源提供布法罗任何其他设施所不提供的服务 该地区的费用是全国最低的。FICSR的主要目标是跟上步伐， 理想情况下，领先于CCSG用户群的细胞计数需求。因此，资源领导将继续关闭 与CCSG成员互动，以确定他们的需求，并将监测新的 技术和分析工具。