Development of a charge-sensitive optical detection system for high-throughput study of small molecules

标题： 用于高通量研究小分子的电荷灵敏光学检测系统的研制 摘要 测量小分子与蛋白质受体结合的动力学和蛋白质的生化反应，如 作为翻译后的蛋白质修饰，是理解疾病的一项基本任务，发现 诊断生物标志物和药物筛选。已经开发了各种无标签技术，但它们的 灵敏度随着分子质量的降低而降低，这使得检测小分子和 生化反应。为了解决这一问题，提出了一种电荷敏感光学检测系统(CSOD 需要。该技术不仅对小分子敏感，而且与标准微孔板兼容 技术，这特别适合于高吞吐量应用。确立了工作原理 并在NIH NCI IMAT(癌症创新分子分析技术)的支持下进行了验证 研究)拨款(R21和R33)给亚利桑那州立大学(ASU)团队。在这个SBIR直接二期项目中， 生物传感仪器公司(BI)将与亚利桑那州立大学的团队合作，开发一种商业上可行的原型系统。 我们将继续与生物医学研究和制药行业的潜在客户合作 用于测试与其兴趣相关的样本，并验证开发系统的性能可用性。 该项目的成功将带来一种新的高通量分子检测筛选技术 相互作用，特别是与蛋白质的小分子相互作用，以及翻译后修饰 蛋白质。这些过程对于生物标记物的发现、疾病诊断和药物筛选都非常重要。

TITLE: Development of a charge sensitive optical detection system for high-throughput study of small molecules SUMMARY Measuring the kinetics of small molecule binding to protein receptors and biochemical reactions of proteins, such as post-translational protein modifications, is a basic task in the understanding of diseases, discovering of diagnosis biomarkers, and screening of drugs. Various label-free techniques have been developed, but their sensitivity decreases with the molecular mass, which makes it challenging to detect small molecules and biochemical reactions. A charge sensitive optical detection (CSOD) system is proposed to address this unmet need. The technology is not only sensitive to small molecules, but also compatible with the standard microplate technology, which is particularly suitable for high-throughput applications. The working principle was established and validated with the support of NIH NCI IMAT (Innovative Molecular Analysis Technologies for cancer research) grants (R21 and R33) to the Arizona State University (ASU) team. In this SBIR direct phase II project, Biosensing Instrument Inc. (BI) will work with the ASU team to develop a commercially viable prototype system. We will continue collaborations with potential customers in biomedical research and pharmaceutical industries for testing samples relevant to their interest and validating the performance usability of the developing system. The success of the project will lead to a new high-throughput screening technology for measuring molecular interactions, particularly small molecule interactions with proteins, and post-translational modifications of proteins. These processes are highly important for biomarker discovery, disease diagnosis and drug screening.