Geobiology of Cataracts

白内障是世界范围内导致失明的主要原因。它们可能是由先天性突变引起的， 透镜蛋白质或包括糖尿病在内的各种疾病。我们最近研究了携带连接蛋白的小鼠 突变（Cx46 fs 380和Cx 50 D47 A），模拟人类白内障相关突变。这些老鼠的晶状体 积累钙，形成沉淀物，对应于白内障。我们假设生物- 矿化是白内障形成的一般机制。我们将描述钙的组成 连接蛋白基因突变小鼠白内障中的白细胞沉淀及其形成与白内障发生的关系。我们 将测试不同透镜蛋白（γ B-晶状体蛋白）突变的小鼠或实验性 诱发的白内障也含有钙沉淀物。这一建议可能会使人们了解基本的 白内障的致病机制，并导致新的预防/治疗方法。

Cataracts are the leading cause of blindness worldwide. They can be caused by congenital mutations of lens proteins or by various diseases including diabetes. We have recently studied mice carrying connexin mutations (Cx46fs380 and Cx50D47A) that mimic human cataract-linked mutations. The lenses of these mice accumulate calcium which forms precipitates that correspond to the cataracts. We hypothesize that bio- mineralization is a general mechanism of cataract formation. We will characterize the composition of calcium precipitates in connexin mutant mice and the relation between their formation and cataract development. We will test whether mice with mutations of a different lens protein (γB-crystallin) or mice with experimentally- induced cataracts also contain calcium precipitates. This proposal may give insights into fundamental pathogenic mechanisms of cataracts and lead to new preventative/therapeutic approaches.