Geobiology of Cataracts

白内障的地球生物学

• 批准号: 10630104
• 负责人: VIVIANA M BERTHOUD BARRANDEGUY
• 金额: $ 54.18万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630104
• 关键词: Animals; Anions; Appearance; Blindness; Buthionine Sulfoximine; Calcium; Calcium ion; Cataract; Cells; Circulation; Confocal Microscopy; Connexins; Crystallins; Darkness; Data; Defect; Deposition; Development; Diabetes Mellitus; Disease; Dyes; Electron Microscopy; Environmental Risk Factor; Etiology; Event; Excision; Functional disorder; Gene Mutation; Genes; Homeostasis; Human; Immunofluorescence Immunologic; In Vitro; Ions; Learning; Lens Fiber; Light; Link; Lipids; Maintenance; Mass Spectrum Analysis; Microscopy; Mus; Mutant Strains Mice; Mutation; Nutrient; Operative Surgical Procedures; Organ; Oxidation-Reduction; Pathogenicity; Pathologic; Patients; Precipitation; Process; Prosthesis; Proteins; Publications; Retina; Risk; Roentgen Rays; Salts; Series; Solubility; Spectrum Analysis; Stains; Streptozocin; Systemic disease; Testing; Therapeutic; Time; Tissues; United States; Visual System; Visual impairment; Water; Western Blotting; X-Ray Computed Tomography; age related; biomineralization; calcification; congenital infection; experimental study; gap junction channel; human model; innovation; insight; intercellular communication; lens; lens transparency; light transmission; microCT; mineralization; mouse model; mutant; new technology; novel; novel therapeutics; operation; prevent; sight restoration

Cataracts are the leading cause of blindness worldwide. They can be caused by congenital mutations of lens proteins or by various diseases including diabetes. We have recently studied mice carrying connexin mutations (Cx46fs380 and Cx50D47A) that mimic human cataract-linked mutations. The lenses of these mice accumulate calcium which forms precipitates that correspond to the cataracts. We hypothesize that bio- mineralization is a general mechanism of cataract formation. We will characterize the composition of calcium precipitates in connexin mutant mice and the relation between their formation and cataract development. We will test whether mice with mutations of a different lens protein (γB-crystallin) or mice with experimentally- induced cataracts also contain calcium precipitates. This proposal may give insights into fundamental pathogenic mechanisms of cataracts and lead to new preventative/therapeutic approaches.

白内障是全世界致盲的主要原因。它们可能是由基因突变引起的