Stanford O'Brien Urology Research Center

斯坦福奥布莱恩泌尿学研究中心

• 批准号: 10297619
• 负责人: JAMES D. BROOKS
• 金额: $ 120万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10297619
• 关键词: 3-Dimensional; Affect; Atlases; Automobile Driving; Benign; Benign Prostatic Hypertrophy; Bioinformatics; Biology; CXCL13 gene; Cell Nucleus; Cell physiology; Cells; Clinical; Collaborations; Communication; Communities; Data; Data Analyses; Development; Dimensions; Disease; Elements; Embryo; Embryonic Development; Ensure; Epithelial; Epithelial Cells; Fibroblasts; Fibrosis; Functional disorder; Gene Expression; Gene Expression Profiling; Goals; Growth; Growth and Development function; Heterogeneity; Histologic; Histology; Human; Image; Immune; Immune response; Immunohistochemistry; Infiltration; Inflammation; Inflammatory; International; Investigation; Lead; Magnetic Resonance Imaging; Modeling; Molecular; Multiplexed Ion Beam Imaging; National Institute of Diabetes and Digestive and Kidney Diseases; Pathogenesis; Pathway interactions; Patients; Pilot Projects; Play; Population; Prostate; Prostatic Diseases; Prostatic Urethra; Radiology Specialty; Reporting; Research; Research Personnel; Resource Sharing; Resources; Role; Sampling; Science; Scientist; Services; Signal Pathway; Signal Transduction; Signaling Molecule; Stromal Cells; Symptoms; Technology; Testing; Therapeutic; Time; Tissue Expansion; Tissues; Training; Urologic Diseases; Urology; associated symptom; base; bioimaging; cell growth; cell type; data de-identification; experience; genomic data; graduate student; histological image; human disease; improved; indexing; lower urinary tract symptoms; man; men; molecular subtypes; next generation; novel therapeutic intervention; novel therapeutics; older men; prevent; programs; prostate enlargement; response; response to injury; senescence; success; three-dimensional modeling; transcriptomics; translational scientist; tumor; tumor-immune system interactions; undergraduate student; urinary; urologic

ABSTRACT – OVERALL COMPONENT Benign prostatic hyperplasia (BPH) is the most common cause of urinary symptoms in older men, yet we understand little about its origins, drivers of growth, and how it causes lower urinary tract symptoms. Since BPH-caused Lower Urinary Tracts Symptoms (LUTS) appears unique to man, we propose a highly integrated project to create an atlas encompassing the molecular, cellular, microenvironmental, histological and macroscopic dimensions of human BPH. Definition of the features responsible for growth and progression of BPH could ultimately lead to new therapeutic approaches to treat or prevent BPH. Our overall goal is to expand research in benign urology to improve our understanding and treatment of urological diseases. The components of the Stanford O’Brien Urology Research Center include: The Administrative Core is based in the Department of Urology and directed by Dr. James Brooks, an experienced clinician and translational scientist in prostate disease who will administer the Center to ensure the scientific and training goals are realized and interface with the NIDDK and Urology Research Consortia. He will be advised by an Internal and External Advisory Board, to ensure progress is made and to provide scientific advice to ensure success. He will meet with the Investigator Committee to formulate plans, integrate findings between projects and allocate Project and Core resources to ensure projects succeed. The Biospecimen/Bioimaging Core, directed by Dr. Robert West provides critical support to projects of the Center by providing human BPH tissues with deidentified data, generates histological images and manages these and the MRI images and provides Multiplexed Ion Beam Imaging (MIBI) and data analysis for Projects 1, 2 & 3. The Core also provides this service to the O’Brien Urology Centers and Urology Disease Centers. Project 1 seeks to define the role of fibroblast subtypes in the development and progression of BPH. Project 2 characterizes the immune microenvironment and investigates how it is shaped by the stromal cells and how it influences the stromal and epithelial compartments of BPH. Project 3 uses MR Images with associated International Prostate Symptom Scores (IPSS) and Bothersome Indices (BI) to construct 3D models of BPH overlayed with histology. These models serve as an atlas for integrating stromal and immune microenvironment data and gene expression subtypes and will provide a means to test how molecular, cellular, microenvironment, histological and radiologic features and their heterogeneity relate BPH to LUTS. These projects serve as the nucleus for training of undergraduate, graduate, and post graduate students to become the next generation of leaders in urological science.

摘要-整体组件 良性前列腺增生（BPH）是老年男性泌尿系统症状的最常见原因，但我们 对它的起源、生长的驱动因素以及它如何引起下尿路症状知之甚少。以来 BPH引起的下尿路症状（LUTS）似乎是人类独有的，我们提出了一个高度综合的 该项目旨在创建一个涵盖分子、细胞、微环境、组织学和生物学的图谱， 人类BPH的宏观尺寸。负责生长和进展的特征的定义 BPH最终可能导致新的治疗方法来治疗或预防BPH。我们的总体目标是 扩大良性泌尿外科的研究，以提高我们对泌尿外科疾病的认识和治疗。的 斯坦福大学奥布莱恩泌尿学研究中心的组成部分包括： 行政核心是设在泌尿科和詹姆斯博士指导布鲁克斯， 经验丰富的临床医生和前列腺疾病的转化科学家，他将管理中心，以确保 实现科学和培训目标，并与NIDDK和泌尿学研究联盟进行对接。他 将由内部和外部咨询委员会提供咨询，以确保取得进展，并提供 科学指导，确保成功。他将与调查委员会会面，制定计划，整合 项目之间的调查结果和分配项目和核心资源，以确保项目的成功。 生物标本/生物成像核心，由罗伯特·韦斯特博士指导，为该项目提供关键支持。 通过为人类BPH组织提供去识别数据，生成组织学图像并管理 这些和MRI图像，并提供多路复用离子束成像（MIBI）和数据分析项目1， 2和3.核心还提供这项服务的奥布莱恩泌尿中心和泌尿疾病中心。 项目1旨在确定成纤维细胞亚型在BPH发展和进展中的作用。 项目2描述了免疫微环境的特征，并研究了基质细胞如何塑造免疫微环境 以及它如何影响BPH的基质和上皮细胞。 项目3使用MR图像以及相关的国际前列腺症状评分（IPSS）和烦恼 指数（BI）构建与组织学重叠的BPH三维模型。这些模型可作为 整合基质和免疫微环境数据和基因表达亚型，并将提供一个 检测分子、细胞、微环境、组织学和放射学特征及其 BPH与LUTS异质性相关。 这些项目作为培养本科生、研究生和研究生的核心， 成为泌尿科学的下一代领导者。