Stanford O'Brien Urology Research Center

抽象-整体组件 良性前列腺增生症(BPH)是老年男性尿路症状的最常见原因，然而我们 对它的起源、生长动力以及它如何引起下尿路症状知之甚少。自.以来 BPH引起的下尿路症状(LUTS)似乎是人类独有的，我们提出了一种高度整合的 创建一份涵盖分子、细胞、微环境、组织学和 人类良性前列腺增生症的宏观维度。定义对儿童的成长和进步负责的特征 BPH最终可能导致治疗或预防BPH的新的治疗方法。我们的总体目标是 扩大良性泌尿外科研究，提高对泌尿外科疾病的认识和治疗水平。这个 斯坦福·奥布莱恩泌尿外科研究中心的组成部分包括： 管理核心设在泌尿科，由詹姆斯·布鲁克斯博士指导，他是 在前列腺病方面经验丰富的临床医生和翻译科学家，他们将管理该中心以确保 科学和培训目标得以实现，并与NIDDK和泌尿外科研究联盟建立了联系。他 将由内部和外部咨询委员会提供咨询，以确保取得进展并提供 确保成功的科学建议。他将与调查委员会会面，制定计划，整合 在项目之间分配结果，并分配项目和核心资源，以确保项目成功。 由Robert West博士指导的Biosecimen/生物成像核心为以下项目提供关键支持 中心通过为人类BPH组织提供识别数据，生成组织图像并管理 这些和MRI图像，并为项目1提供多路复用离子束成像(MIBI)和数据分析， 2和3.Core也向O‘Brien泌尿中心和泌尿外科疾病中心提供这项服务。 项目1试图确定成纤维细胞亚型在良性前列腺增生症的发生和发展中的作用。 项目2描述了免疫微环境的特征，并研究了基质细胞如何塑造免疫微环境。 以及它如何影响BPH的间质和上皮室。 项目3使用具有相关国际前列腺症状评分(IPSS)和麻烦的磁共振图像 指数(BI)，以构建与组织学重叠的BPH三维模型。这些模型可作为地图集 整合间质和免疫微环境数据以及基因表达亚型，并将提供 测试分子、细胞、微环境、组织学和放射学特征以及它们的 异质性将BPH与LUTS联系起来。 这些项目是培养本科生、研究生和研究生的核心 成为下一代泌尿外科科学的领导者。

ABSTRACT – OVERALL COMPONENT Benign prostatic hyperplasia (BPH) is the most common cause of urinary symptoms in older men, yet we understand little about its origins, drivers of growth, and how it causes lower urinary tract symptoms. Since BPH-caused Lower Urinary Tracts Symptoms (LUTS) appears unique to man, we propose a highly integrated project to create an atlas encompassing the molecular, cellular, microenvironmental, histological and macroscopic dimensions of human BPH. Definition of the features responsible for growth and progression of BPH could ultimately lead to new therapeutic approaches to treat or prevent BPH. Our overall goal is to expand research in benign urology to improve our understanding and treatment of urological diseases. The components of the Stanford O’Brien Urology Research Center include: The Administrative Core is based in the Department of Urology and directed by Dr. James Brooks, an experienced clinician and translational scientist in prostate disease who will administer the Center to ensure the scientific and training goals are realized and interface with the NIDDK and Urology Research Consortia. He will be advised by an Internal and External Advisory Board, to ensure progress is made and to provide scientific advice to ensure success. He will meet with the Investigator Committee to formulate plans, integrate findings between projects and allocate Project and Core resources to ensure projects succeed. The Biospecimen/Bioimaging Core, directed by Dr. Robert West provides critical support to projects of the Center by providing human BPH tissues with deidentified data, generates histological images and manages these and the MRI images and provides Multiplexed Ion Beam Imaging (MIBI) and data analysis for Projects 1, 2 & 3. The Core also provides this service to the O’Brien Urology Centers and Urology Disease Centers. Project 1 seeks to define the role of fibroblast subtypes in the development and progression of BPH. Project 2 characterizes the immune microenvironment and investigates how it is shaped by the stromal cells and how it influences the stromal and epithelial compartments of BPH. Project 3 uses MR Images with associated International Prostate Symptom Scores (IPSS) and Bothersome Indices (BI) to construct 3D models of BPH overlayed with histology. These models serve as an atlas for integrating stromal and immune microenvironment data and gene expression subtypes and will provide a means to test how molecular, cellular, microenvironment, histological and radiologic features and their heterogeneity relate BPH to LUTS. These projects serve as the nucleus for training of undergraduate, graduate, and post graduate students to become the next generation of leaders in urological science.