Revealing novel pathogenic and repairing mechanisms of CADASIL disease.

揭示 CADASIL 疾病的新致病和修复机制。

• 批准号: 10419211
• 负责人: LI-RU ZHAO
• 金额: $ 49.52万
• 依托单位: UPSTATE MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10419211
• 关键词: Adult; Affect; Antibodies; Area; Arterial Disorder; Arteries; Automobile Driving; Biological; Biological Assay; Blood - brain barrier anatomy; Blood Circulation; Blood Vessels; Blood capillaries; Blood flow; Bone Marrow; Brain; Cancer Patient; Cells; Cerebral small vessel disease; Cerebrovascular Disorders; Cerebrum; Cessation of life; Combined Modality Therapy; Data; Defect; Dementia; Deposition; Disease; Disease Progression; Extracellular Domain; Extravasation; FDA approved; Functional disorder; Future; Gene Mutation; Genes; Genetic; Granulocyte Colony-Stimulating Factor; Growth Factor; Hematopoietic Cell Growth Factors; Image; Immunoglobulin G; Impaired cognition; Impairment; Induced Mutation; Inherited; Intervention; Ischemic Stroke; Knowledge; Methods; Microvascular Dysfunction; Missense Mutation; Molecular; Mus; Mutation; NOTCH3 gene; Pathogenesis; Pathogenicity; Pathologic; Pathology; Patients; Pericytes; Pharmacology; Play; Recovery; Recurrence; Research; Research Priority; Role; Route; Signal Transduction; Smooth Muscle Myocytes; Stem Cell Factor; Stroke; Subcortical Infarctions; Subcortical Leukoencephalopathy; Testing; Therapeutic; Thrombosis; Toxic effect; Transgenic Mice; Treatment Factor; United States National Institutes of Health; Up-Regulation; VEGFA gene; Vascular Dementia; Vascular Endothelial Growth Factors; Vascular Smooth Muscle; base; bevacizumab; cellular pathology; cerebral capillary; cerebrovascular; cerebrovascular pathology; chemotherapy; density; disability; enhancing factor; improved; innovation; insight; middle age; mortality; mouse model; neuron loss; novel; preclinical study; receptor; recombinase-mediated cassette exchange; repaired; stem cells; therapeutic development; transcriptome sequencing; two photon microscopy; two-photon; vascular cognitive impairment and dementia

PROJECT SUMMARY/ABSTRACT Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) represents the most common form of hereditary ischemic stroke (microinfarcts) and vascular dementia. CADASIL is a monogenic cerebral small vessel disease driven by progressive degeneration of mural cells due to NOTCH3 gene mutation in the mural cells (vascular smooth muscle cells and pericytes). This vascular degeneration-induced dementia is one of the diseases sharing a similar pathological mechanism of “Vascular Contributions to Cognitive Impairment and Dementia” (VCID). The NIH has designated VCID as a critical research area requiring studies to explore new molecular mechanisms of cerebrovascular pathology and potential treatments for vascular dementia. CADASIL mainly affects young and middle-aged adults and causes severe disability and early death. No treatment is available to stop or delay disease progression for CADASIL. Although the causative role of NOTCH3 gene mutation in CADASIL was revealed in 1996, it still remains unclear today how NOTCH3 mutation drives cerebrovascular defects. The lack of this pathogenic knowledge creates a critical barrier for the development of therapeutic strategies for CADASIL. Our earlier studies have demonstrated that combined treatment of two hematopoietic growth factors, stem cell factor (SCF) and granulocyte colony-stimulating factor (G-CSF) (SCF+G-CSF) ameliorates vascular smooth muscle cell degeneration, cerebrovascular regression, capillary thrombosis, microinfarcts, blood-brain barrier (BBB) leakage, and cognitive impairments in a mouse model of CADASIL (TgNotch3R90C mice). It remains to be determined, however, how the SCF+G-CSF treatment enhances cerebrovascular repair in TgNotch3R90C mice. The objective of this application is to determine a novel pathogenic mechanism for CADASIL and the underlying mechanism for SCF+G-CSF-enhanced cerebrovascular repair in TgNotch3R90C mice. Using 2- photon cortical capillary circulation imaging, BBB integrity assay, pharmacological intervention, and Cre-Lox technology, this application will define how impaired cerebral capillary circulation in TgNotch3R90C mice is improved by SCF+G-CSF treatment (Aim 1), how cerebral capillary leakage in TgNotch3R90C mice is ameliorated by SCF+G-CSF treatment (Aim 2), and how mural cell-produced pathogenic molecules drive progressive cerebrovascular defects and how SCF+G-CSF treatment ameliorates the cerebrovascular defects by modulating the pathogenic molecules (Aim 3) in TgNotch3R90C mice. It is expected that the findings of this mechanistic study will significantly advance the understanding of the pathogenesis for CADASIL and will reveal new targets and new routes to develop novel treatments for CADASIL. This proposed study fits within the research priorities of NIH-highlighted VCID research.

项目总结/文摘