Shared Resource: Biosample Repository Facility

共享资源：生物样本储存库设施

• 批准号: 10427539
• 负责人: DENISE C CONNOLLY
• 金额: $ 4.92万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10427539
• 关键词: Abbreviations; Advisory Committees; Basic Science; Bioinformatics; Biological; Biological Markers; Biological Models; Biological Specimen Banks; Biometry; Blood; Blood Cells; Blood Donations; Blood specimen; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cancer Control; Cell Culture Techniques; Cell Separation; Clinical; Clinical Data; Collaborations; Collection; Comprehensive Cancer Center; Computer Assisted; Consent; Custom; Detection; Development; Development Plans; Diagnostic; Doctor of Philosophy; Drug or chemical Tissue Distribution; Enzyme-Linked Immunosorbent Assay; Epigenetic Process; Ethics; Family Cancer History; Family member; Farming environment; Fox Chase Cancer Center; Foxes; Freezing; Funding; Future; Genomics; Goals; Health; Health system; Histopathology Facility; Hospital Personnel; Human; Immunoperoxidase Technics; Individual; Informed Consent; Institutional Review Boards; Interleukin-6; International; Intervention; Laboratory Animals; Link; Malignant - descriptor; Malignant Neoplasms; Medical Oncologist; Molecular; Normal tissue morphology; Participant; Pathologic; Pathologist; Pathology; Patient Self-Report; Patients; Peer Review; Population Study; Procedures; Process; Recording of previous events; Research; Research Personnel; Research Support; Resource Sharing; Resources; Sampling; Services; Specimen; Stains; Supervision; Surgeon; Surgical Pathology; Therapeutic; Tissue Banks; Tissue Microarray; Tissue Sample; Tissues; Training; Transgenic Mouse Facility; Translational Research; Universities; University Hospitals; Urine; Work; animal facility; automated image analysis; cancer prevention; clinical database; high throughput screening; imaging facilities; imaging system; lifestyle factors; liquid crystal polymer; medical schools; member; molecular modeling; patient oriented; peripheral blood; programs; quantitative imaging; research study; volunteer

MODEL SYSTEMS SHARED RESOURCE: BIOSAMPLE REPOSITORY FACILITY (BRF) PROJECT SUMMARY/ABSTRACT Fox Chase Cancer Center (FCCC) established biospecimen banking activities in 1987 to coordinate the ethical collection, storage, annotation and distribution of tissue and peripheral blood samples to support translational research. This centralized shared resource operates under the supervision of Denise Connolly, PhD (MT) and is advised by an inter-programmatic Facility Advisory Committee (FAC). To enhance BRF services, the Center invested $369,770 for a PE Vectra Multispectral Quantitative Imaging System and a MesoScale Discovery chemiluminescent ELISA platform. The Biosample Repository Facility (BRF) has three major functions: 1) to identify participants, obtain informed consent, collect tissue, blood and/or urine specimens and obtain information on personal and family histories of cancer, clinical intervention and lifestyle factors for use in research; 2) to process and store human biospecimens obtained through investigator-initiated studies; and 3) to provide tissue-related analysis services including custom tissue microarray (TMA) construction, standard immunoperoxidase and fluorescent immunohistochemical detection and analysis of biomarker expression. Working in collaboration with pathologists, medical oncologists, surgeons and other hospital personnel, specifically trained BRF staff obtains subject informed consent, collects samples and assembles comprehensive clinical, pathological and personal/demographic information about each donor and the corresponding samples. As part of this banking process, the BRF staff provides: 1) diagnostic pathology support; 2) a comprehensive informed consent process for the appropriate collection of tissue, blood and other biospecimens for research; 3) a specialized biospecimen bank devoted to the collection and distribution of de- identified specimens to support research; 4) custom TMA construction; 5) immunohistochemical staining; 6) computer assisted tissue analysis services; and 7) extensive supporting data from the clinical record and self- reported health history of each participant. All specimens collected by and housed in the BRF are linked to comprehensive clinical databases supported by the Population Studies Facility (PSF). The BRF currently houses over 16,500 fresh-frozen tissue samples, over 47,000 blood samples, and has access to >100,000 surgical pathology cases. The annual accruals for new tissue and blood specimens are approximately 1,500 and 3,000, respectively. In 2014, the BRF served 31 FCCC investigators (24 peer-review funded) across all five Research Programs. Ninety (90%) percent of the BRF's 2014 use (by primary service line) was by peer-review funded Cancer Center members. Future plans involve the Center's commitment to construct a dedicated freezer farm that will provide sample storage capacity for the next ten years.

模型系统共享资源：生物样本库设施(BRF) 项目摘要/摘要 狐狸大通癌症中心于1987年建立了生物质谱库活动，以协调伦理 组织和外周血液样本的收集、存储、注释和分发，以支持翻译 研究。这种集中的共享资源在Denise Connolly博士(MT)和 由方案间融资机制咨询委员会(FAC)提供咨询。为加强博鳌亚洲论坛服务，中心 投资369,770美元用于PE Vectra多光谱定量成像系统和中尺度发现 化学发光酶联免疫吸附试验平台。生物样本库设施(BRF)有三个主要功能：1) 确定参与者的身份，获得知情同意，收集组织、血液和/或尿液样本，并获得 关于癌症的个人和家族病史、临床干预和生活方式因素的信息 研究；2)处理和储存通过研究人员发起的研究获得的人类生物样本；以及3) 提供组织相关分析服务，包括定制组织微阵列(TMA)构建、标准 免疫过氧化物酶和荧光免疫组织化学检测和分析生物标志物的表达。 与病理学家、内科肿瘤学家、外科医生和其他医院工作人员合作， 经过专门培训的BRF工作人员获得受试者知情同意，采集样本和组装 关于每个捐赠者的全面临床、病理和个人/人口统计信息 相应的样本。作为银行业务流程的一部分，基金会工作人员提供：1)诊断病理学 支持；2)全面的知情同意程序，以便适当收集组织、血液和其他 用于研究的生物质谱库；3)专门的生物质谱库，致力于收集和分发脱氧核糖核酸 确定支持研究的样本；4)定制TMA结构；5)免疫组织化学染色；6) 计算机辅助组织分析服务；以及7)来自临床记录和自我检查的广泛支持数据 报告每个参与者的健康史。所有由基金会收集和存放的标本都与 人口研究机构(PSF)支持的全面临床数据库。目前，BRF 储存了超过16,500份新鲜冷冻组织样本，超过47,000份血液样本，并可以获得100,000份 外科病理病例。新的组织和血液样本的年应计费用约为1500美元 和3000人。2014年，论坛为31名《气候变化框架公约》调查员提供了服务(24名同行审查资助) 五个研究项目。BRF 2014年使用量的90%(通过主要服务项目)是 同行评议资助的癌症中心成员。未来的计划包括该中心致力于建设一个 专门的冷冻场，将为未来十年提供样品存储能力。