ISU VDL Capacity Building and Methods Development Grant

项目摘要 食用动物饲料或伴侣动物饲料的污染不仅会伤害动物， 但也会产生对人体有害的残留物。例如，在2007年的三聚氰胺食品爆发中， 宠物食品中的黑色素检测先于婴儿配方奶粉中的黑色素检测， 中国的孩子们。因此，快速检测动物食品/饲料中的毒素可以保护公众健康。的 2007年的三聚氰胺食品爆发暴露了兽医诊断实验室能力的许多不足， 在紧急情况下快速检测和量化食品污染。这导致成立，6-7年 前，Vet-LIRN，一个由联邦，州和大学实验室组成的网络，其功能是催化 开发新的和快速的基于组织的分析技术，提供设备齐全和人员配备齐全的 设施，提高合作伙伴关系的有效性，以协调食品安全和国防资源，以减少 食源性疾病的发病率，并增加网络实验室之间的沟通。爱荷华州 大学兽医诊断实验室（ISU VDL）是创始成员和热心支持者， 参与网络活动。尽管这打破了单个实验室的“筒仓”心态， 尽管沟通有所改善，但在保护动物健康和公众健康方面仍有许多工作要做。这 项目有两个具体目标： 具体目标1：执行支助股兽医诊断实验室的能力建设。为了提高我们的 实验室，我们需要保持我们的设备和工作人员在“准备”模式，以快速，有效地响应 VPO的电话。我们建议购买设备，支付服务协议，为我们的实验室提供差旅费 参加FDA会议和继续教育的人员，保持我们对Vet-LIRN活动的参与 例如实验室间能力测试活动。我们将支持大规模的浪涌容量测试 动物食品/饲料紧急事件，并参与确定的短期监测和相关活动 由VPO我们还将参与来自其他实验室的测试的实验室间验证。 具体目标#2：开发和验证用于诊断钠的基于组织的诊断方法 动物一氟乙酸灭鼠剂中毒。故意污染食用牲口的食物， 杀鼠剂会损害动物的健康和像爱荷华州这样的农业州的经济。美国 政府已将一氟乙酸钠确定为优先研究的杀虫剂，因为： （a）它有可能被用作化学武器， B）它没有解毒剂，并且与人类的高死亡率和高发病率相关 和动物知识差距是一个主要问题，本提案将解决这一问题， 缺乏针对这种毒物的快速的基于组织的诊断方法。执行支助股-虚拟数据库将牵头开展工作， 基于常见技术平台（即HPLC荧光和/或离子色谱）的组织分析方法 层析我们建议对基于组织的分析方法进行实验室内和实验室间验证。 从这个提议中发展起来的方法。 如果获得资助，ISU VDL将保持作为Vet-LIRN实验室的全部功能，并随时准备为以下方面做出贡献： FDA履行《食品安全现代化法案》要求的使命。

PROJECT SUMMARY Contamination of feed for food producing animals or food for companion animals not only can harm animals, but can also cause residues that can harm people. For example, in the melamine food outbreak of 2007, detection of melanine in pet food preceded detection of the same in infant formula which sickened and killed children in China. Therefore, rapid detection of toxins in animal food/feed can protect public health. The melamine food outbreak of 2007 revealed many deficiencies in veterinary diagnostic laboratory capacity to rapidly detect and quantify food contamination during emergency outbreaks. This led to founding, 6-7 years ago, of Vet-LIRN, a network of Federal, State, and University Laboratories whose function is to catalyze the development of new and rapid tissue-based analytical techniques, provide for well-equipped and well-staffed facilities, improve the effectiveness of partnerships to coordinate food safety and defense resources to reduce the incidence of foodborne illnesses, and to increase communication among network labs. The Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) is a founding member and an avid supporter and participant in network activities. Whereas this has broken down the “silo” mentality of individual labs and improved communication, a lot more remains to be done to protect animal health and public health. This project proposal has two Specific Aims: Specific Aim #1: Capacity building of the ISU Veterinary Diagnostic Laboratory. To improve readiness of our laboratory we need to keep our equipment and staff in a “ready” mode to quickly and efficiently respond to VPO’s calls. We propose to purchase equipment, pay for service agreements, fund travel for our laboratory personnel to attend FDA meetings and continuing education, maintain our participation in Vet-LIRN activities such as inter-laboratory proficiency testing exercises. We will support surge capacity testing during large scale animal food/feed emergency events and participate in short-term surveillance and related activities identified by the VPO. We will also participate in inter-laboratory validation of tests originating from other labs. Specific Aim #2: To develop and validate tissue-based diagnostic method(s) for diagnosis of sodium monofluoroacetate rodenticide intoxication in animals. Intentional contamination of food for food animals by this rodenticide can harm both the health of the animals and the economy of agricultural states like Iowa. The US government has identified sodium monofluoroacetate as a priority pesticide for research because: a) It potentially could to be used as a chemical weapon, and b) There is no antidote to it and it is associated with a high mortality and high morbidity rates both in humans and animals. The knowledge gap, which is a major concern and is to be addressed by this proposal, is that rapid tissue-based diagnostic methods for this toxicant are lacking. The ISU-VDL will lead the effort to develop tissue-based analytical methods based on common technology platforms ie HPLC fluorescence and/or Ion Chromatography. We propose to conduct both intra-lab-and inter-lab validation of tissue-based analytical methods developed from this proposal. If funded, the ISU VDL will maintain full functionality as a Vet-LIRN laboratory, with readiness to contribute to the FDA mission in fulfilment of requirements of the Food Safety Modernization Act.