ISU VDL Capacity Building and Methods Development Grant

ISU VDL 能力建设和方法开发补助金

• 批准号: 10424404
• 负责人: WILSON Kiiza RUMBEIHA
• 金额: $ 6.36万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10424404
• 关键词: ISU; VDL; Capacity; Building; Methods

PROJECT SUMMARY Contamination of feed for food producing animals or food for companion animals not only can harm animals, but can also cause residues that can harm people. For example, in the melamine food outbreak of 2007, detection of melanine in pet food preceded detection of the same in infant formula which sickened and killed children in China. Therefore, rapid detection of toxins in animal food/feed can protect public health. The melamine food outbreak of 2007 revealed many deficiencies in veterinary diagnostic laboratory capacity to rapidly detect and quantify food contamination during emergency outbreaks. This led to founding, 6-7 years ago, of Vet-LIRN, a network of Federal, State, and University Laboratories whose function is to catalyze the development of new and rapid tissue-based analytical techniques, provide for well-equipped and well-staffed facilities, improve the effectiveness of partnerships to coordinate food safety and defense resources to reduce the incidence of foodborne illnesses, and to increase communication among network labs. The Iowa State University Veterinary Diagnostic Laboratory (ISU VDL) is a founding member and an avid supporter and participant in network activities. Whereas this has broken down the “silo” mentality of individual labs and improved communication, a lot more remains to be done to protect animal health and public health. This project proposal has two Specific Aims: Specific Aim #1: Capacity building of the ISU Veterinary Diagnostic Laboratory. To improve readiness of our laboratory we need to keep our equipment and staff in a “ready” mode to quickly and efficiently respond to VPO’s calls. We propose to purchase equipment, pay for service agreements, fund travel for our laboratory personnel to attend FDA meetings and continuing education, maintain our participation in Vet-LIRN activities such as inter-laboratory proficiency testing exercises. We will support surge capacity testing during large scale animal food/feed emergency events and participate in short-term surveillance and related activities identified by the VPO. We will also participate in inter-laboratory validation of tests originating from other labs. Specific Aim #2: To develop and validate tissue-based diagnostic method(s) for diagnosis of sodium monofluoroacetate rodenticide intoxication in animals. Intentional contamination of food for food animals by this rodenticide can harm both the health of the animals and the economy of agricultural states like Iowa. The US government has identified sodium monofluoroacetate as a priority pesticide for research because: a) It potentially could to be used as a chemical weapon, and b) There is no antidote to it and it is associated with a high mortality and high morbidity rates both in humans and animals. The knowledge gap, which is a major concern and is to be addressed by this proposal, is that rapid tissue-based diagnostic methods for this toxicant are lacking. The ISU-VDL will lead the effort to develop tissue-based analytical methods based on common technology platforms ie HPLC fluorescence and/or Ion Chromatography. We propose to conduct both intra-lab-and inter-lab validation of tissue-based analytical methods developed from this proposal. If funded, the ISU VDL will maintain full functionality as a Vet-LIRN laboratory, with readiness to contribute to the FDA mission in fulfilment of requirements of the Food Safety Modernization Act.

项目总结 生产动物的饲料或同伴动物的食物受到污染不仅会伤害动物， 但也会造成残留物，对人体造成伤害。例如，在2007年三聚氰胺食品暴发事件中， 宠物食品中的黑色素检测先于婴儿配方奶粉中的黑色素检测，婴儿配方奶粉中的黑色素导致疾病和死亡 中国的孩子们。因此，快速检测动物性食品/饲料中的毒素可以保护公众健康。这个 2007年爆发的三聚氰胺食品暴露出兽医诊断实验室在能力上的许多不足 在紧急疫情期间快速检测和量化食品污染。这导致了成立，6-7年 Vet-LIRN是一个由联邦、州和大学实验室组成的网络，其功能是催化 新的和快速的基于组织的分析技术的发展，提供了设备齐全和人员齐全的 设施，提高伙伴关系的有效性，协调食品安全和国防资源，以减少 食源性疾病的发病率，并增加网络实验室之间的交流。爱荷华州 大学兽医诊断实验室(ISU VDL)是创始成员和热心支持者， 网络活动的参与者。鉴于这打破了个别实验室和 改善沟通，在保护动物健康和公共健康方面仍有很多工作要做。这 项目建议书有两个具体目标： 具体目标1：执行支助股兽医诊断实验室的能力建设。为了提高我们的 实验室我们需要使我们的设备和工作人员处于“随时准备”的状态，以便快速有效地应对 VPO的电话。我们建议购买设备，支付服务协议，资助我们实验室的旅费 参加FDA会议和继续教育的人员，保持我们对VET-LIRN活动的参与 例如实验室间的能力测试活动。我们将支持大规模期间的浪涌容量测试 动物性食品/饲料紧急事件，并参与确定的短期监测和相关活动 被VPO带走了。我们还将参与来自其他实验室的测试的实验室间验证。 特定目的#2：建立和验证钠中毒的组织学诊断方法(S) 动物中的单氟乙酸酯杀鼠剂中毒。故意污染食用动物的食物 杀鼠剂既会损害动物的健康，也会损害爱荷华州等农业州的经济。美国 政府已将一氟乙酸钠确定为优先研究的杀虫剂，原因是： A)它有可能被用作化学武器，以及 B)没有解毒剂，它与人类的高死亡率和高发病率有关 还有动物。知识差距是一个主要问题，这项提案将解决这一问题，即 这种毒物缺乏基于组织的快速诊断方法。ISU-VDL将领导开发工作 基于常用技术平台的组织分析方法，如高效液相色谱、荧光和/或离子 层析法。我们建议同时进行基于组织的分析的实验室内和实验室间的验证 从这一提议发展出来的方法。 如果得到资助，ISU VDL将保持作为Vet-LIRN实验室的全部功能，并随时准备为 FDA的使命是履行《食品安全现代化法案》的要求。