Vet-LIRN Network Capacity-Building Project Molecular Gel Imaging system at UI VDL

工程摘要 伊利诺伊大学（UI）兽医诊断实验室（VDL）是一个全方位的服务，AAVLD认证，所有 物种，参考兽医诊断实验室。作为VET-LIRN的成员，UI VDL是其中一个实验室 积极参与多项VET-LIRN活动，包括作为牵头实验室的李斯特菌iSeq测序项目， 作为源实验室为MiSeq WGS测序项目提供分离株，参与抗生素耐药性研究 试点项目，并作为牵头实验室评估自动核酸提取方法。凝胶图像的质量 对于传统的分子PCR是至关重要的，我们目前的凝胶成像系统， 很旧，因此产生低质量的图像。在这份提案中，我们要求拨款购买一台Bio-Rad GelDoc Go凝胶成像系统，用于提高UI VDL分子服务质量，以检测动物疾病，以及 Vet-LIRN协调的常规病例调查和监测。

Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference veterinary diagnostic laboratory. As a member of the VET-LIRN, UI VDL is one of the labs actively involved in several VET-LIRN activities including iSeq-sequencing Listeria project as a lead lab, providing isolates for MiSeq WGS sequencing project as a source lab, participation on antimicrobial resistance pilot project, and evaluation of automatic nucleic acid extraction methods as a lead lab. The quality of gel images for conventional molecular PCRs are critical in making call on the results and our current gel imaging system is very old, thus producing low quality of images. In this proposal, we request funding to purchase a Bio-Rad GelDoc Go Gel Imaging System to enhance UI VDL molecular service quality for testing animal diseases, and routine case investigation and surveillance coordinated by Vet-LIRN.