Vet-LIRN Network Capacity-Building Project Molecular Gel Imaging system at UI VDL

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Project Abstract The University of Illinois (UI) Veterinary Diagnostic Laboratory (VDL) is a full service, AAVLD accredited, all species, reference veterinary diagnostic laboratory. As a member of the VET-LIRN, UI VDL is one of the labs actively involved in several VET-LIRN activities including iSeq-sequencing Listeria project as a lead lab, providing isolates for MiSeq WGS sequencing project as a source lab, participation on antimicrobial resistance pilot project, and evaluation of automatic nucleic acid extraction methods as a lead lab. The quality of gel images for conventional molecular PCRs are critical in making call on the results and our current gel imaging system is very old, thus producing low quality of images. In this proposal, we request funding to purchase a Bio-Rad GelDoc Go Gel Imaging System to enhance UI VDL molecular service quality for testing animal diseases, and routine case investigation and surveillance coordinated by Vet-LIRN.