Transrenal DNA for point-of-care diagnosis of TB
用于结核病即时诊断的经肾 DNA
基本信息
- 批准号:10443886
- 负责人:
- 金额:$ 18.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-02 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:AdultAerosolsBase PairingBiological AssayCellsCessation of lifeChildChildhoodClinicalClinical SensitivityCollectionCommunicable DiseasesCoughingDNADetectionDevelopmentDiagnosisDiseaseExhibitsExposure toFoundationsGenomic SegmentGoldGrowthHIVHIV SeronegativityHIV SeropositivityHIV/TBIndividualKidneyLengthLibrariesLow incomeLungMethodsMicroscopyMucous body substanceMycobacterium tuberculosisOutcome StudyPatientsPersonsPopulationPreparationPulmonary TuberculosisRecoveryResourcesSample SizeSamplingSensitivity and SpecificitySouth AfricaSpecificitySpecimenSputumTestingTimeTuberculosisTuberculosis diagnosisUnited StatesUrineWorkaccurate diagnosisbaseblood filterco-infectioncohortdesigndiagnostic accuracydisease diagnosiseffective therapyhuman DNAimprovedlaboratory developmentlaboratory facilitylow and middle-income countriesmultiplex assaymultiplex detectionnon-tuberculosis mycobacterianovelpilot testpoint of carepoint of care testingpoint-of-care diagnosistuberculosis diagnosticstuberculosis treatment
项目摘要
ABSTRACT
M. tuberculosis (Mtb) infects one-third of the world’s population, causes TB disease in 10 million people per year,
and is responsible for 1.6 million deaths annually. While effective treatments for TB have been available for over
50 years, a major barrier to its control has remained the inability to reliably diagnose disease in low-resource
TB-endemic settings. Most clinical tests require an adequate sample of sputum (mucus from the lungs) which
may be difficult to obtain in patients without a cough, in children, and in HIV-infected individuals. Urine offers an
attractive method for point-of-care TB diagnostics since it is abundant, simple to collect, and collection does not
involve exposure to infectious aerosols. Transrenal DNA (trDNA) present in the urine offers a specific target that
can be amplified for high sensitivity. The development of a PCR-based test of TB from urine, and its development
as a point-of-care test, could have enormous impact on TB diagnostics.
Aim 1. To improve detection of Mtb-specific trDNA using ultrashort PCR and multiplexing. Hypothesis:
Amplification of shorter fragments and/or targeting multiple unique fragments will enable detection of low-
concentrations samples, increasing sensitivity while maintaining specificity.
Aim 2. To determine sensitivity and specificity of improved Mtb-specific trDNA detection in urine
samples from adults and children in a TB-endemic setting. Hypothesis: Mtb trDNA is present in detectable
quantities in urine from both adults and children with pulmonary TB regardless of HIV status, and our
hybridization sequence-capture method can increase clinical sensitivity compared to previous TB trDNA studies.
抽象的
结核病分枝杆菌(MTB)感染世界人口的三分之一,每年引起1000万人结核病,
并每年造成160万人死亡。虽然已经有效的结核病治疗可以结束
50年,其控制的主要障碍仍然无法可靠地诊断出低资源的疾病
TB-endomic设置。大多数临床测试都需要足够的痰液样本(肺部的粘液)
在没有咳嗽,儿童和艾滋病毒感染者的患者中可能难以获得。尿液提供
护理点结核病诊断的有吸引力的方法,因为它丰富,收集易于收集,收集不
涉及暴露于传染性气溶胶。尿液中存在的跨肾脏DNA(trDNA)提供了一个特定的靶标
可以扩增以获得高灵敏度。开发了尿液中基于PCR的结核病测试及其发育
作为一项护理点测试,可能会对结核病诊断物产生巨大影响。
AIM 1。使用Ultrashort PCR和多路复用来提高MTB特异性TRDNA的检测。假设:
较短片段和/或靶向多个独特片段的扩增将使低 - 检测
浓度样本,提高灵敏度,同时保持特异性。
目标2。确定尿液中MTB特异性TrDNA检测的灵敏度和特异性
在结核病环境中的成人和儿童的样本。假设:MTB trDNA在可检测的
不论艾滋病毒状况如何
与以前的TB TrDNA研究相比,杂交序列捕获方法可以提高临床灵敏度。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Barry Ryan Lutz其他文献
Barry Ryan Lutz的其他文献
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{{ truncateString('Barry Ryan Lutz', 18)}}的其他基金
Transrenal DNA for point-of-care diagnosis of TB
用于结核病即时诊断的经肾 DNA
- 批准号:
10285973 - 财政年份:2021
- 资助金额:
$ 18.29万 - 项目类别:
Engineering a denaturant-resistant polymerase for direct nucleic acid diagnostics
设计用于直接核酸诊断的抗变性聚合酶
- 批准号:
10308721 - 财政年份:2020
- 资助金额:
$ 18.29万 - 项目类别:
V-OLA: point-of-care HIV viral load monitoring and drug resistance testing
V-OLA:即时 HIV 病毒载量监测和耐药性测试
- 批准号:
10407553 - 财政年份:2019
- 资助金额:
$ 18.29万 - 项目类别:
V-OLA: point-of-care HIV viral load monitoring and drug resistance testing
V-OLA:即时 HIV 病毒载量监测和耐药性测试
- 批准号:
10179314 - 财政年份:2019
- 资助金额:
$ 18.29万 - 项目类别:
V-OLA: point-of-care HIV viral load monitoring and drug resistance testing
V-OLA:即时 HIV 病毒载量监测和耐药性测试
- 批准号:
10655323 - 财政年份:2019
- 资助金额:
$ 18.29万 - 项目类别:
HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoring
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- 批准号:
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HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoring
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- 批准号:
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- 资助金额:
$ 18.29万 - 项目类别:
HIV-specific target capture and quantitative isothermal amplification for acute HIV diagnosis and treatment monitoring
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- 批准号:
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$ 18.29万 - 项目类别:
Point-of-care diagnosis of pulmonary TB from urine samples
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- 批准号:
9166566 - 财政年份:2016
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$ 18.29万 - 项目类别:
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