Natural Sources and Microbial Transformation of Marine Halogenated Pollutants

海洋卤化污染物的天然来源和微生物转化

• 批准号: 10443787
• 负责人: Eric Ellsworth Allen
• 金额: $ 12.56万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10443787
• 关键词: Anabolism; Animal Model; Animals; Aquaculture; Area; Bacteria; Bacterial Genes; Biochemical; Biochemistry; Biota; Chemicals; Climate; Collaborations; Consumption; Coupled; Cytochrome P450; Dioxins; Environment; Enzymes; Farm; Fishes; Flame Retardants; Fluorescent in Situ Hybridization; Food Chain; Food Webs; Genes; Genetic; Genomics; Habitats; Health; Herbicides; Human; In Vitro; Industrialization; Insecticides; Institution; Isomerase; Joints; Laboratories; Lead; Logic; Mammals; Marine Invertebrates; Mentorship; Metabolic; Metabolic Biotransformation; Metagenomics; Microbe; Mixed Function Oxygenases; Molecular; Monitor; Movement; National Institute of Environmental Health Sciences; Natural Products; Oceanography; Pathway interactions; Pharmaceutical Preparations; Phenols; Polychlorinated Biphenyls; Production; Proteobacteria; Pyrroles; Recording of previous events; Research; Resolution; Risk; Route; Ryanodine Receptor Calcium Release Channel; Seafood; Source; Tetrachlorodibenzodioxin; Thyroid Hormone Receptor; Toxic effect; Toxin; Work; agent orange; anthropogenesis; base; bioaccumulation; dibenzo(1,4)dioxin; dietary; food consumption; in vivo; man; metabolomics; metagenomic sequencing; microbial; microbiome; microbiota; novel; persistent organic pollutants; pollutant; polybrominated diphenyl ether; public health relevance; structural biology; transcriptome; transcriptomics

Project Summary/Abstract Natural polybrominated organic compounds such as hydroxylated polybrominated diphenyl ethers (OH-- BDEs) and polybrominated pyrroles (PBPs) have recently emerged as chemicals of human health concern. These natural product relatives of anthropogenic halogenated persistent organic pollutants (POPs) are widely distributed throughout the marine food web and accumulate in seafood sources consumed by humans. We and others have demonstrated that OH--BDEs such as 6--OH--BDE--47 (thyroid hormone receptor) and PBPs such as tetrabromopyrrole (ryanodine receptor) are potent toxins and thus pose a potential risk to humans. Many fundamental questions however remain about the extent of sources for these natural organobromine molecules, how these chemicals enter and move through the marine food web, whether changes in the climate will impact their production and accumulation, and whether humans are more or less impacted by natural halogenated POPs versus their anthopogentic counterparts. Recent discoveries by the Moore and Allen laboratories have rigorously established the genetic and biochemical basis for the microbial synthesis of natural OH--BDE molecules in diverse lineages of marine bacteria. However, the global distribution and ubiquity of these polybrominated POPs in marine biota cannot be fully explained by the sources discovered thus far, suggesting additional biogenic sources exist and are actively contributing to OH- -BDE and MeO--BDE accumulation in the marine food web. This information is critical to more accurately identify trophic connections and interconversions that lead to natural PBDE accumulation in marine fish and ultimately, human dietary exposure risks. In this project, new genetic and biochemical evidence for the biosynthesis and biotransformation of PBDE molecules will be established for marine macroalgae, a conspicuous but uncharacterized source of PBDE molecules in marine habitats, using transcriptome analysis coupled with biochemical enzyme characterization. Additional microbial sources for PBDE synthesis/transformation will be characterized by the comprehensive analysis of fish and marine-- mammal associated microbiomes using integrated genomic and metabolomic approaches combined with experimental microbiome enrichment reactors amended with PBDE molecules or biosynthetic substrates. The proposed work will be undertaken jointly by the laboratories of Moore (biochemistry) and Allen (genomics) at SIO who have a proven track record of collaboration and joint mentorship in these areas.

项目摘要/摘要 天然多溴的有机化合物，例如羟基化的多溴二苯基醚（OH--- BDES）和多溴吡咯（PBP）最近出现了作为人类健康关注的化学物质。 这些天然产物的亲戚是人为卤化的持续有机污染物（POP）的亲戚 广泛分布在整个海洋食品网中，并积聚在人类消费的海鲜来源中。 我们和其他人已经证明了OH--BDE，例如6-oh---bde-47（甲状腺激素受体） PBP（例如四翼吡俄（Ryanodine受体））是有效的毒素，因此构成潜在的风险 人类。然而，许多基本问题仍然是关于这些自然来源的程度 有机粒细胞分子，这些化学物质如何进入和移动海洋食品网络，无论是 气候变化将影响他们的生产和积累，以及人类是否或多或少 受到天然卤素流行音乐的影响与其触角同行的影响。最近发现 摩尔和艾伦实验室已经严格建立了微生物的遗传和生化基础 海洋细菌多种谱系中天然OH-BDE分子的合成。但是，全球 这些多溴在海洋生物群中的分布和普遍存在不能完全解释 到目前为止发现的来源，表明存在其他生物源，并积极促进OH- -bde和Meo-bde在海洋食品网中积累。此信息对于更准确 识别营养连接和互连，导致海洋鱼类中自然的PBDE积累 最终，人类饮食暴露风险。在这个项目中，新的遗传和生化证据 PBDE分子的生物合成和生物转化将建立用于海洋大量藻类（A） 使用转录组分析 结合生化酶的表征。 PBDE的其他微生物来源 合成/转化的特征是对鱼类和海洋的全面分析 - 哺乳动物 使用集成的基因组和代谢组方法结合实验的相关微生物组 用PBDE分子或生物合成底物修改的微生物组富集反应器。提议 摩尔实验室（生物化学）和艾伦（基因组学）将共同开展工作 在这些领域拥有合作和共同指导的良好记录。

项目成果

Herbivorous Fish Microbiome Adaptations to Sulfated Dietary Polysaccharides

• DOI: 10.1128/aem.02154-22
• 发表时间: 2023-05
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: S. Podell;A. Oliver;L. Kelly;Wesley J. Sparagon;Alvaro M. Plominsky;R. Nelson;L. Laurens;Simona Augy

Impacts of the Marine Hatchery Built Environment, Water and Feed on Mucosal Microbiome Colonization Across Ontogeny in Yellowtail Kingfish, Seriola lalandi

• DOI: 10.3389/fmars.2021.676731
• 发表时间: 2021-05
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: J. Minich;B. Nowak;A. Elizur;R. Knight;S. Fielder;E. Allen

Temporal, Environmental, and Biological Drivers of the Mucosal Microbiome in a Wild Marine Fish, Scomber japonicus

• DOI: 10.1128/msphere.00401-20
• 发表时间: 2019-08
• 期刊: mSphere
• 影响因子: 4.8
• 作者: J. Minich;Semar Petrus;J. Michael;T. Michael;R. Knight;E. Allen

A genomic view of trophic and metabolic diversity in clade-specific Lamellodysidea sponge microbiomes

• DOI: 10.1186/s40168-020-00877-y
• 发表时间: 2020-06-23
• 期刊: MICROBIOME
• 影响因子: 15.5
• 作者: Podell, Sheila;Blanton, Jessica M.;Allen, Eric E.
• 通讯作者: Allen, Eric E.

Enzymatic Halogenation of Terminal Alkynes.

• DOI: 10.1021/jacs.3c05750
• 发表时间: 2023-08-30
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Lukowski, April L.;Hubert, Felix M.;Ngo, Thuan-Ethan;Avalon, Nicole E.;Gerwick, William H.;Moore, Bradley S.
• 通讯作者: Moore, Bradley S.