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A novel method for improved separation of metagenomic samples for preservation and identification.

一种改进宏基因组样本分离以保存和鉴定的新方法。

基本信息

批准号：
10324639
负责人：
Michael Marciano
金额：
$ 21.88万
依托单位：
SYRACUSE BIOLABS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2023-03-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10324639
关键词：
Bacteria Benchmarking Bioinformatics Biological Blood Cells Chemicals Crime Cytolysis DNA DNA Packaging DNA Sequence DNA analysis DNA purification DNA sequencing Data Detection Development Diagnosis Ensure Filtration Forensic Medicine Growth Health Healthcare Human Human Microbiome Individual Institutes Intelligence Legal patent Manufacturer Name Market Research Metagenomics Methods Microbe Microbiology National Security Nucleic Acids Organism Outcome Phase Phylogenetic Analysis Plant DNA Plants Protocols documentation Reagent Research Sales Saliva Sampling Science Scientist Security Small Business Technology Transfer Research Techniques Technology Tissues United States National Institutes of Health Universities base cost effective detection limit improved innovation interest microbial microbial community microbial host microbiome microbiome research novel novel diagnostics preservation prevent sensor technology success

项目摘要

Abstract The success of all microbiome-based studies are highly dependent on effective sample purification of the target organisms of interest. Characterizing individual organismal components of these metagenomic samples relies heavily upon the ability to separate the individual phylogenetic components, such as removing human cells from the microbiological fraction while preventing samples loss or degradation. This Phase I STTR project aims to significantly advance DNA purification methods of metagenomics / environmental samples (e.g. human and bacteria) using a patent pending simple and inexpensive purification approach aimed at effectively separating and preserving host and microbial DNA. This method will ensure all organismal DNA is preserved (i.e. not “sacrificed” or loss) during initial separation steps, unlike protocols employed by existing DNA purification kit manufacturers. Outcomes should result in higher quality DNA sequence data (i.e. higher sequencing coverage and higher sensitivity) thus providing robust information content, leading to higher confidence in the organismal characterization. Syracuse Biolabs, Inc. (SBL), has teamed with scientists in the Forensic and National Security Sciences Institute (FNSSI) at Syracuse University to further improve and commercialize this novel DNA purification approach. This Phase I project has three aims directed to establish a proof-of-concept of the method leading to initial kit development for fieldable DNA purification modules of metagenomic samples. Our metagenomics DNA purification kit is also applicable to fields outside of the human microbiome research market, including forensic identification and geolocation of biological samples relevant to criminal and intelligence related activities, DNA based ecological research, plant and microbial based geosourcing and defense/homeland security efforts (requiring DNA purification from environmental samples).

摘要