Improving iPSC reprogramming and CRISPR gene editing workflows and efficacy using CellRaft technology
使用 CellRaft 技术改进 iPSC 重编程和 CRISPR 基因编辑工作流程和功效
基本信息
- 批准号:10324993
- 负责人:
- 金额:$ 25.66万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-01 至 2023-07-31
- 项目状态:已结题
- 来源:
- 关键词:AcademiaAdherent CultureAutomationCapitalCase StudyCell Culture TechniquesCell LineCell SeparationCell SurvivalCellsCellular MorphologyClinicalClinical TrialsClone CellsClustered Regularly Interspaced Short Palindromic RepeatsConsumptionCustomDNA Sequence AlterationDermalDevelopmentDiseaseDissociationElectroporationEnsureEquipmentEvaluationFibroblastsGenesGeneticHarvestHumanImageIndustryInvestmentsLaboratoriesMaintenanceManualsMeasuresMethodologyMethodsMicroscopeMicroscopicMonitorMorphologyNaturePainPatientsPhasePhenotypePlaguePolystyrenesPopulationProcessProtocols documentationPublishingResearch PersonnelSeminalSickle Cell AnemiaSorting - Cell MovementStainsSystemTechniquesTechnologyTherapeuticTimeTissuesViralVisualizationbasebeta Thalassemiacell immortalizationcell typecostcost effectivecost efficientculture platesdrug developmentdrug discoveryfeedinggene therapyimprovedindividual patientinduced pluripotent stem cellinstrumentationmicrosystemsnext generation sequencingpersonalized medicinepluripotencyprecision drugsprogramsstem cell growthstem cellssuccesstool
项目摘要
Project Summary
Induced pluripotent stem cells (iPSCs) have become a commonly used tool to generate multiple cell types from
a given individual patient. Reprogramming non-invasively harvested cells, such as dermal fibroblasts, into
iPSCs, allows investigators to generate any cell type from a patient with known genetic and clinical backgrounds.
This method has been particularly powerful for cases in which a disease condition manifests in a tissue where
acquiring patient-derived primary cells is challenging, or there are genetic mutations. However, the workflows
associated with reprogramming, CRISPR gene editing, and differentiating iPSCs are low-throughput, costly,
time-consuming, and not supported by commercially available automated instrumentation. Customized
automation systems have been published and generally require considerable capital investment, multiple types
of equipment, custom programming, and commitment to a single reprogramming method. There is an unmet
need in academia and industry for technology that can improve the efficiency and success rates of a)
reprogramming, b) iPSC viability, and c) the ability to generate clonally derived CRISPR edited cell lines in an
automated fashion. The development of streamlined workflows on a single platform that can deliver higher
efficiencies in iPSC reprogramming and cell line development is likely to dramatically enhance the utility and
throughput of the technology, thereby accelerating the use of iPSCs in personalized medicine and drug
discovery. Cell Microsystems proposes the use of the CellRaft Technology, comprising the automated AIR
System and CytoSort Array cell culture consumable as a cost-effective, automated, and gentle solution that can
solve many of the pain points that plague these critical workflows. In this Phase I program, we will develop
protocols for the culture, reprogramming, and editing of iPSCs using our proprietary consumable and
instrumentation and demonstrate that our technology provides faster, more cost effective, and more efficient
workflows for iPSC cloning. Commercially, there is a tremendous potential market in laboratories across all
industries that perform these workflows, and our company has a proven successful track record in developing
tools for the drug discovery market.
项目摘要
诱导性多能干细胞(iPSC)已经成为一种常用的工具,
一个特定的病人。将非侵入性收获的细胞,如真皮成纤维细胞,
iPSC允许研究人员从具有已知遗传和临床背景的患者中产生任何细胞类型。
该方法对于其中疾病状况在组织中表现的情况特别有效,其中
获取患者来源的原代细胞具有挑战性,或者存在基因突变。然而,工作流
与重编程、CRISPR基因编辑和分化iPSC相关的基因编辑是低通量的,昂贵的,
耗时,并且不受商业上可获得的自动化仪器的支持。定制
自动化系统已经公开,并且通常需要相当大的资本投资、多种类型
设备,自定义编程,并致力于一个单一的重新编程方法。存在未满足的
学术界和工业界对能够提高效率和成功率的技术的需求a)
重编程的能力,B)iPSC活力,和c)在重组细胞中产生克隆衍生的CRISPR编辑的细胞系的能力。
自动化的方式。在单一平台上开发简化的工作流程,
iPSC重编程和细胞系开发的效率可能会大大提高其效用,
该技术的吞吐量,从而加速iPSC在个性化医疗和药物中的使用
的发现Cell Microsystems提出使用CellRaft技术,包括自动化AIR
系统和CytoSort Array细胞培养耗材是一种经济高效、自动化和温和的解决方案,
解决困扰这些关键工作流程的许多痛点。在第一阶段,我们将开发
使用我们专有的耗材和材料,
仪器,并证明我们的技术提供更快,更经济,更有效的
iPSC克隆的工作流程。在商业上,所有实验室都有巨大的潜在市场。
执行这些工作流程的行业,我们公司在开发方面有着成功的记录,
药物发现市场的工具。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jessica Hartman其他文献
Jessica Hartman的其他文献
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{{ truncateString('Jessica Hartman', 18)}}的其他基金
Rapid identification and selection of functional antigen-specific monoclonalantibodies by FcGR-enabled screening on CellRaft Arrays in the CellRaft AIRSystem
通过在 CellRaft AIRSystem 中的 CellRaft 阵列上进行 FcGR 筛选,快速鉴定和选择功能性抗原特异性单克隆抗体
- 批准号:
10698784 - 财政年份:2021
- 资助金额:
$ 25.66万 - 项目类别:
Three-dimensional organoid culture using the CellRaft microwell technology
使用 CellRaft 微孔技术进行三维类器官培养
- 批准号:
10227800 - 财政年份:2020
- 资助金额:
$ 25.66万 - 项目类别:
Three-dimensional organoid culture using the CellRaft microwell technology
使用 CellRaft 微孔技术进行三维类器官培养
- 批准号:
10081103 - 财政年份:2020
- 资助金额:
$ 25.66万 - 项目类别:
High Throughput CRISPR/Cas9 cell line generation using the CellRaft Array
使用 CellRaft 阵列生成高通量 CRISPR/Cas9 细胞系
- 批准号:
9910418 - 财政年份:2017
- 资助金额:
$ 25.66万 - 项目类别:
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