Probe-type nanowire sensor for label-free, in-situ, ultrasensitive detection of biomarkers from single cell in adherent culture

我们的研究小组开发了一种微型绘图设备，化学笔，它可以使用有限的开放空间层流在纳米尺度上控制表面上局部区域的溶液。我们发展了一种开放空间层流法，在正常条件下精确地制备单根金纳米线。这种方法使我们能够制造金纳米线的宽度范围为200纳米到几十微米，长度范围为3毫米或更少的目标位置。金纳米线是由多个纳米粒子聚集成线状结构。所制备的金纳米线被证明是一个很好的伊加生物传感与极低的检测限（1 aM）。因此，这种新技术有望在广泛的领域中应用。这是首次在正常环境下精确制备金纳米线。与银纳米线相比，金纳米线在生物传感方面的应用更为广泛。在本研究中，使用单个金纳米线实现了高性能的伊加检测，然而，通过根据目标物质改变金纳米线的修饰，我们期望开发出能够在药理学、医疗保健、食品分析、环境分析甚至感染诊断领域中以高灵敏度测量各种物质的传感器。

Our research group has developed a micro drawing device, the chemical pen, which enables to control solution in local area on surfaces at the nanoscale using confined open-space laminar flow. We have developed an open-space laminar flow approach for fabricating a single gold nanowire at precise position under normal condition. This method enabled us to fabricate gold nanowires with the widths ranged of 200 nm to several ten micrometers and lengths ranged of 3 mm or less at targeted locations. The gold nanowires were composed of multiple nanoparticles aggregated into a wire-like structure. The fabricated gold nanowire was demonstrated an excellent biosensing for IgA with an extremely low limit of detection (1 aM). Thus, this novel technique is expected to be applied in a wide range of fields. It is the first time to fabricate gold nanowires at precise position and in normal environment. Compare to Ag nanowire, gold nanowire is more widely used for biosensing. The high-performance IgA detection was achieved using a single gold nanowire in this study, however, by changing the modification of the gold nanowires according to the target substance, we expect to develop a sensor that can measure various substances with high sensitivity in the field of pharmacology, medical care, food analysis, environmental analysis and even of infection diagnose.