COVID-19: High Efficiency SARS-CoV-2 Virus Aerosol Sampling and Sensing

COVID-19：高效 SARS-CoV-2 病毒气溶胶采样和传感

• 批准号: 10325899
• 负责人: Patricia Bea Keady
• 金额: $ 26.28万
• 依托单位: AEROSOL DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-06 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10325899
• 关键词: 2019-nCoV; Abate; Aerosols; Air; Antibodies; Assisted Living Facilities; Bacillus anthracis; Bacteria; Binding; Biosensor; Bluetooth; Bordetella pertussis; Breathing; Buffers; COVID-19; COVID-19 detection; COVID-19 pandemic; Collaborations; Collection; Colorado; Consumption; Coughing; Decontamination; Desiccation; Detection; Devices; Disease; Disease Outbreaks; Early Diagnosis; Electrodes; Ensure; Environment; Exhalation; Exposure to; Future; General Population; Goals; Growth; Health Personnel; Health care facility; Hospitals; Hour; Human; Individual; Influenza; Influenza B Virus; Intervention; Label; Laboratories; Liquid substance; Long-Term Care; Lung; Measles; Mechanical Stress; Methods; Military Personnel; Molecular Analysis; Monitor; Nucleic Acids; Nursing Homes; Outcome; Particle Size; Patients; Phase; Physical condensation; Play; Prisons; RNA; Reading; Recovery; Reporting; Resolution; Reverse Transcriptase Polymerase Chain Reaction; SARS-CoV-2 antibody; SARS-CoV-2 infection; SARS-CoV-2 spike protein; SARS-CoV-2 transmission; Sampling; Small Business Technology Transfer Research; Social Impacts; Spectrum Analysis; Sterility; Structure; Surface; System; Techniques; Technology; Testing; Time; Transportation; Tube; Universities; Vial device; Virion; Virus; Vulnerable Populations; Weight; Work; aerosolized; base; cost; detection limit; detection method; detection platform; economic impact; electric impedance; improved; innovation; instrument; operation; pandemic disease; particle; pathogen; pathogenic virus; point of care; prevent; professor; prototype; respiratory; respiratory pathogen; sensor; temporal measurement; tool; transmission process; viral RNA; viral detection; virology

As the current COVID-19 pandemic has demonstrated, aerosolized pathogens such as SARS-CoV-2 can spread explosively if not quickly detected. Early detection of these airborne pathogens can help control out- breaks, particularly within vulnerable populations in densely populated spaces including assisted living facili- ties, places of worship, military installations, hospitals, and prisons. To date, most studies of aerosolized SARS-CoV-2 use filter collection followed by laboratory analysis of viral RNA. While this gives a general indica- tion of viral RNA levels in an environment, filters collect both infectious and non-infectious viruses and damage them through mechanical stress and desiccation, making it difficult to determine the infective fraction of the col- lected viruses. Further, current filter analysis methods require time-consuming nucleic acid extraction and am- plification techniques not suitable to rapid, point-of-care monitoring. Therefore, there is an urgent need for in- struments that can detect intact viruses at the point-of-care. We propose to develop a sensitive, direct reading, bioaerosol detection platform that can quantify specific air- borne pathogens at the point of collection. Furthermore, if a positive result is identified, the sample can be taken to a central laboratory for additional molecular analysis and infectivity testing. This device will integrate the bioaerosol collection technology developed at Aerosol Devices Inc. (ADev) with virus detection technology from Colorado State University (CSU). Using gentle condensation-growth capture that mimics the human lung, the ADev sampler will be the front-end of the platform, concentrating intact, viable virus particles into a small liquid volume. With >90% collection efficiency for particle sizes <10 nm up to 10 µm, ADev samplers uniformly collect all inhalable SARS-CoV-2 particles whether originating from cough droplets (>5 µm), smaller particles exhaled during breathing or talking (<5 µm), or shed as individual virus particles (~120 nm). CSU Professors Henry, Dandy, and Geiss are developing innovative, inexpensive electrochemical biosensors to rapidly detect intact viruses in liquid samples. By synergizing these robust sensors with the ADev aerosol sampler, we will produce a system that can detect and quantify ultra-low concentrations of viral pathogens in near real-time. In this phase I STTR project, we will adapt our commercial aerosol-into-liquid collector to incorporate CSU’s biosensors. Specific aims for the ADev sampler include extending sampling time to 24 h to enhance limits of detection, increasing volumetric sampling rate to improve temporal resolution, selecting materials compatible with VHP decontamination and optimizing the system to reduce size, weight, power consumption and cost. Specific aims for the biosensor include modifying electrodes to detect SARS-CoV-2 particles with high sensitiv- ity and selectivity, adapting our current electrode configuration to work with the ADev sampling vial, and testing the system with infectious SARS-CoV-2 in our BSL-3 laboratory. Our immediate focus is SARS-CoV-2, but this technology can be adapted in Phase II to detect any airborne pathogen (e.g., Influenza, B. anthracis, etc.).

正如目前的新冠肺炎大流行所表明的那样，像SARS-CoV-2这样的气雾化病原体可以 如果不能迅速发现，就会爆炸性地传播。及早发现这些空气传播的病原体可以帮助控制- 中断，特别是在人口稠密空间的脆弱人群中，包括辅助生活设施- 领带、礼拜场所、军事设施、医院和监狱。到目前为止，大多数关于雾化的研究 SARS-CoV-2使用过滤采集，然后进行病毒RNA的实验室分析。虽然这给出了一个一般的指标- 环境中病毒RNA水平的变化，过滤器收集感染性和非感染性病毒和损害 通过机械应力和干燥，使得很难确定胶原的感染分数。 选定的病毒。此外，目前的滤器分析方法需要耗时的核酸提取和AM-2。 扩增技术不适用于快速的护理点监测。因此，迫切需要-- 可在护理地点检测完整病毒的仪器。 我们建议开发一种灵敏的、直接读取的生物气溶胶检测平台，该平台可以量化特定的空气- 在采集点携带病原体。此外，如果确定结果为阳性，则可以将样本 被带到中心实验室进行额外的分子分析和传染性测试。这台设备将集成 气溶胶设备公司(ADev)开发的具有病毒检测技术的生物气溶胶收集技术 来自科罗拉多州立大学(CSU)。使用模仿人类肺部的温和凝结生长捕捉， ADev采样器将是平台的前端，将完好无损的、可存活的病毒颗粒浓缩到一个小的 液体体积。具有&gt；90%的收集效率，粒度为&lt；10 nm至10微米，ADev采样器均匀 收集所有可吸入的SARS-CoV-2颗粒物，无论是来自咳嗽飞沫(&gt；5微米)，更小的颗粒物 在呼吸或说话时呼出(&lt；5微米)，或以单个病毒颗粒的形式脱落(~120纳米)。加州州立大学教授 亨利、丹迪和盖斯正在开发创新的、廉价的电化学生物传感器，以快速检测 液体样本中的完整病毒。通过将这些坚固耐用的传感器与ADev气溶胶采样器协同工作，我们将 生产一种系统，可以近乎实时地检测和量化超低浓度的病毒病原体。 在这个第一阶段的STTR项目中，我们将改装我们的商用气溶胶到液体的收集器，以纳入CSU的 生物传感器。ADev采样器的具体目标包括将采样时间延长至24小时，以提高对 检测，提高体积采样率以提高时间分辨率，选择兼容的材料 采用VHP去污和优化系统，以降低体积、重量、功耗和成本。 该生物传感器的具体目标包括修饰电极以检测高灵敏度的SARS-CoV-2颗粒。 稳定性和选择性，调整我们当前的电极配置以与ADev取样瓶配合使用，并进行测试 我们BSL-3实验室的传染性SARS-CoV-2系统。我们目前关注的焦点是SARS-CoV-2，但这 可以在第二阶段采用技术来检测任何空气传播的病原体(例如流感、炭疽杆菌等)。