Direct-read monitor for airborne viral pathogens

空气传播病毒病原体的直读监测仪

• 批准号: 9770710
• 负责人: Patricia Bea Keady
• 金额: $ 27.93万
• 依托单位: AEROSOL DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-18 至 2020-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9770710
• 关键词: Accident and Emergency department; Address; Aerosols; Agreement; Air; Amines; Animals; Antibodies; Antigens; Bacteria; Bacteriophage M13; Biological; Biotin; Businesses; Carbon; Collection; Colorado; Communicable Diseases; Containment; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electrochemistry; Electrodes; Ensure; Enzyme-Linked Immunosorbent Assay; Epidemic; Feedback; Food; Goals; Growth; Ha antigen; Hospitals; Hour; Human; Image; In Situ; Incubators; Infection; Influenza A virus; Inhalation; Laboratories; Licensing; Liquid substance; Loudness; Lung; Methods; Microfluidic Microchips; Microfluidics; Monitor; Nucleic Acids; Nursing Homes; Paper; Particulate; Phase; Physical condensation; Polymerase Chain Reaction; Polymethyl Methacrylate; Population; Process; Public Health; Reading; Sampling; Site; Skin; Small Business Technology Transfer Research; Spectrum Analysis; Surface; System; Systems Integration; Technology; Testing; Time; Universities; Viral; Viral Antigens; Virion; Virus; Vulnerable Populations; Water; Work; absorption; aerosolized; base; cost; cost effective; design; disease transmission; electric impedance; influenzavirus; innovation; innovative technologies; instrument; meetings; microchip; multiplex detection; nanoparticle; novel; novel strategies; particle; pathogen; pathogenic virus; portability; response; sensor; transmission process; treatment center; vector; viral detection

Aerosolized pathogens, such as Influenza viruses, can spread rapidly and silently throughout a population, causing severe outbreaks that can be difficult to control. Early detection of these airborne pathogens is key to controlling outbreaks, especially among vulnerable populations such as in hospitals, treatment centers or nursing homes. Yet available methods are neither rapid, nor portable. Current methods rely heavily on off-site laboratory analysis with detection using enzyme-linked immunosorbent assays (ELISA) to detect pathogen antigens and/or polymerase chain reaction (PCR) to detect pathogen nucleic acids in collected samples. This two-step process, collection followed by off-line laboratory analysis, significantly increases effort and time-to- detection. Moreover, traditional ELISA and PCR are complicated, and not amenable to automated detection of emerging outbreaks at the point of contact. Additionally, currently used bioaerosol collection methods tend to damage viral particles, making subsequent detection of viral antigens and nucleic acids difficult. These factors make current bioaerosol collection and detection systems suboptimal, and further renders them intractable for automated, real-time detection. Needed is a sensitive, portable, direct-reading, bioaerosol detection platform that can detect specific pathogens at the point of contact. Such an instrument would enable quick identification of outbreaks, and thereby enable rapid response to public health threats. Aerosol Devices Inc. is an early stage startup company that is developing an innovative bioaerosol collection system that is quiet, fully integrated, and inexpensive relative to traditional bioaerosol sampling systems. Using a gentle condensation collection approach that mimics the human lung, it produces high-quality, concentrated, small-volume liquid samples of viable bioaerosol pathogens that are ideally suited for microfluidic sensors. Likewise, Drs. Henry, Dandy, and Geiss at Colorado State University are actively developing innovative microfluidic electrochemical sensors to rapidly detect intact virus particles in biological samples. The electrochemical sensors are robust, inexpensive, and can provide near real-time feedback of the presence of ultralow concentrations of viral pathogens when used with liquid samples. Combining these two technologies will provide a unique solution for bioaerosol detection that addresses the current critical market need. In this Phase I STTR proposal, we will demonstrate a proof-of-principle system that combines the two innovative technologies to enable real-time detection of aerosolized viral particles. The proposed system synergizes Colorado State University strengths in infectious diseases, electrochemistry, and microfluidics with Aerosol Devices expertise in rapid and robust aerosol collection towards a single fully integrated system that can rapidly detect aerosolized pathogens, providing early warning of outbreaks and helping to save lives.

像流感病毒这样的气雾化病原体可以在人群中迅速而无声地传播， 导致严重的疫情，可能很难控制。及早发现这些空气传播的病原体是关键 控制疫情，特别是在脆弱人群中，如医院、治疗中心或 养老院。然而，现有的方法既不快速，也不便携。目前的方法严重依赖于非现场 用酶联免疫吸附试验(ELISA)检测病原体的实验室分析 抗原和/或聚合酶链式反应(PCR)检测采集样本中的病原体核酸。这 两步流程，收集，然后离线实验室分析，显著增加了工作量和时间，以- 侦测。另外，传统的酶联免疫吸附试验和聚合酶链式反应都比较复杂，不适合自动化检测。 在接触点出现新的疫情。此外，目前使用的生物气溶胶收集方法往往 破坏病毒颗粒，使随后检测病毒抗原和核酸变得困难。这些因素 使目前的生物气溶胶收集和检测系统变得次优，并进一步使它们难以解决 自动、实时检测。需要一种灵敏、便携、直读的生物气溶胶检测平台 可以在接触点检测到特定的病原体。这样的工具将使快速识别成为可能 预防暴发，从而能够对公共卫生威胁作出快速反应。 气溶胶设备公司是一家早期初创公司，正在开发一种创新的生物气溶胶收集 与传统的生物气溶胶采样系统相比，该系统安静、完全集成且价格低廉。vbl.使用 一种温和的冷凝收集方法，模仿人类的肺，它产生高质量的，浓缩的， 适用于微流控的活体生物气溶胶病原体的小体积液体样本 传感器。同样，科罗拉多州立大学的亨利、丹迪和盖斯博士也在积极开发 创新的微流控电化学传感器，可快速检测生物样本中的完整病毒颗粒。这个 电化学传感器坚固耐用，价格低廉，可以提供近乎实时的反馈 当与液体样本一起使用时，病毒病原体的浓度极低。将这两种技术结合起来 将为生物气溶胶检测提供一种独特的解决方案，满足当前关键的市场需求。 在这个第一阶段的STTR提案中，我们将演示一个将两者相结合的原则证明系统 能够实时检测雾化病毒颗粒的创新技术。拟议的系统 将科罗拉多州立大学在传染病、电化学和微流体方面的优势与 气溶胶设备公司在快速和强大的气溶胶收集方面的专业知识，使其成为一个完全集成的系统， 可以快速检测气雾化病原体，对疫情提供早期预警，并帮助拯救生命。