Direct-read monitor for airborne viral pathogens

空气传播病毒病原体的直读监测仪

• 批准号: 9770710
• 负责人: Patricia Bea Keady
• 金额: $ 27.93万
• 依托单位: AEROSOL DEVICES, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-18 至 2020-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9770710
• 关键词: Accident and Emergency department; Address; Aerosols; Agreement; Air; Amines; Animals; Antibodies; Antigens; Bacteria; Bacteriophage M13; Biological; Biotin; Businesses; Carbon; Collection; Colorado; Communicable Diseases; Containment; Deposition; Detection; Development; Devices; Disease Outbreaks; Early Diagnosis; Electrochemistry; Electrodes; Ensure; Enzyme-Linked Immunosorbent Assay; Epidemic; Feedback; Food; Goals; Growth; Ha antigen; Hospitals; Hour; Human; Image; In Situ; Incubators; Infection; Influenza A virus; Inhalation; Laboratories; Licensing; Liquid substance; Loudness; Lung; Methods; Microfluidic Microchips; Microfluidics; Monitor; Nucleic Acids; Nursing Homes; Paper; Particulate; Phase; Physical condensation; Polymerase Chain Reaction; Polymethyl Methacrylate; Population; Process; Public Health; Reading; Sampling; Site; Skin; Small Business Technology Transfer Research; Spectrum Analysis; Surface; System; Systems Integration; Technology; Testing; Time; Universities; Viral; Viral Antigens; Virion; Virus; Vulnerable Populations; Water; Work; absorption; aerosolized; base; cost; cost effective; design; disease transmission; electric impedance; influenzavirus; innovation; innovative technologies; instrument; meetings; microchip; multiplex detection; nanoparticle; novel; novel strategies; particle; pathogen; pathogenic virus; portability; response; sensor; transmission process; treatment center; vector; viral detection

Aerosolized pathogens, such as Influenza viruses, can spread rapidly and silently throughout a population, causing severe outbreaks that can be difficult to control. Early detection of these airborne pathogens is key to controlling outbreaks, especially among vulnerable populations such as in hospitals, treatment centers or nursing homes. Yet available methods are neither rapid, nor portable. Current methods rely heavily on off-site laboratory analysis with detection using enzyme-linked immunosorbent assays (ELISA) to detect pathogen antigens and/or polymerase chain reaction (PCR) to detect pathogen nucleic acids in collected samples. This two-step process, collection followed by off-line laboratory analysis, significantly increases effort and time-to- detection. Moreover, traditional ELISA and PCR are complicated, and not amenable to automated detection of emerging outbreaks at the point of contact. Additionally, currently used bioaerosol collection methods tend to damage viral particles, making subsequent detection of viral antigens and nucleic acids difficult. These factors make current bioaerosol collection and detection systems suboptimal, and further renders them intractable for automated, real-time detection. Needed is a sensitive, portable, direct-reading, bioaerosol detection platform that can detect specific pathogens at the point of contact. Such an instrument would enable quick identification of outbreaks, and thereby enable rapid response to public health threats. Aerosol Devices Inc. is an early stage startup company that is developing an innovative bioaerosol collection system that is quiet, fully integrated, and inexpensive relative to traditional bioaerosol sampling systems. Using a gentle condensation collection approach that mimics the human lung, it produces high-quality, concentrated, small-volume liquid samples of viable bioaerosol pathogens that are ideally suited for microfluidic sensors. Likewise, Drs. Henry, Dandy, and Geiss at Colorado State University are actively developing innovative microfluidic electrochemical sensors to rapidly detect intact virus particles in biological samples. The electrochemical sensors are robust, inexpensive, and can provide near real-time feedback of the presence of ultralow concentrations of viral pathogens when used with liquid samples. Combining these two technologies will provide a unique solution for bioaerosol detection that addresses the current critical market need. In this Phase I STTR proposal, we will demonstrate a proof-of-principle system that combines the two innovative technologies to enable real-time detection of aerosolized viral particles. The proposed system synergizes Colorado State University strengths in infectious diseases, electrochemistry, and microfluidics with Aerosol Devices expertise in rapid and robust aerosol collection towards a single fully integrated system that can rapidly detect aerosolized pathogens, providing early warning of outbreaks and helping to save lives.

雾化病原体，例如流感病毒，可以在人群中快速、无声地传播， 造成难以控制的严重爆发。及早发现这些空气传播的病原体是关键 控制疫情，特别是在医院、治疗中心或医院等弱势群体中 疗养院。然而现有的方法既不快速也不便携。目前的方法严重依赖场外 使用酶联免疫吸附测定 (ELISA) 进行实验室分析和检测来检测病原体 抗原和/或聚合酶链反应（PCR）来检测收集样本中的病原体核酸。这 两步过程，收集后进行离线实验室分析，显着增加了工作量和时间 检测。此外，传统的ELISA和PCR技术较为复杂，不适合自动化检测。 接触点正在爆发疫情。此外，目前使用的生物气溶胶收集方法往往 破坏病毒颗粒，使得后续病毒抗原和核酸的检测变得困难。这些因素 使当前的生物气溶胶收集和检测系统不够理想，并进一步使其难以处理 自动化、实时检测。需要一个灵敏、便携式、直读的生物气溶胶检测平台 可以在接触点检测特定病原体。这样的仪器将能够快速识别 爆发，从而能够快速应对公共卫生威胁。 Aerosol Devices Inc. 是一家早期初创公司，正在开发创新的生物气溶胶系列 相对于传统的生物气溶胶采样系统，该系统安静、完全集成且价格低廉。使用 一种模仿人肺的温和冷凝收集方法，它产生高质量、浓缩、 活生物气溶胶病原体的小体积液体样品，非常适合微流体 传感器。同样，博士。科罗拉多州立大学的 Henry、Dandy 和 Geiss 正在积极开发 创新的微流体电化学传感器，可快速检测生物样本中完整的病毒颗粒。这 电化学传感器坚固耐用、价格低廉，并且可以提供近乎实时的反馈 与液体样品一起使用时，病毒病原体浓度超低。结合这两种技术 将为生物气溶胶检测提供独特的解决方案，满足当前的关键市场需求。 在第一阶段 STTR 提案中，我们将演示一个结合了两者的原理验证系统 能够实时检测雾化病毒颗粒的创新技术。拟议的系统 协同科罗拉多州立大学在传染病、电化学和微流体学方面的优势 Aerosol Devices 在快速、强大的气溶胶收集方面拥有专业知识，旨在打造一个完全集成的系统， 可以快速检测雾化病原体，提供疫情爆发早期预警并帮助拯救生命。