Microbiome and Transcriptomics Core (MTC)

微生物组和转录组学核心 (MTC)

• 批准号: 10341203
• 负责人: Allison Faye Gillaspy
• 金额: $ 20.71万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10341203
• 关键词: Applications Grants; Area; Bioinformatics; Cells; Centers of Research Excellence; Consultations; Core Facility; Cytometry; Data; Data Analyses; Ensure; Experimental Designs; Future; Gene Expression; Gene Expression Profiling; Genomics; Human Resources; Immunity; Infection; Knowledge; Laboratories; Manuscripts; Methods; Molecular Biology; Oklahoma; Pathogenesis; Preparation; Process; Protocols documentation; RNA; Research; Research Personnel; Role; Sampling; System; Technology; Training; Universities; data submission; experience; instrument; microbial; microbiome; microbiome research; next generation sequencing; pathogen; response; tool; transcriptome sequencing; transcriptomics

ABSTRACT (Microbiome and Transcriptomics Core - MTC) Current studies in the area of microbial pathogenesis and immunity require knowledge of whole cell and system wide responses to infection of both the host and pathogen. Without proper expertise in state of the art technology and analysis tools necessary to gather this information, progress cannot be made at the pace required for remaining at the forefront of scientific discovery. The Microbiome and Transcriptomics Core (MTC) will serve an integral role in ensuring that our COBRE researchers can properly collect microbiome and gene expression data at the highest level of rigor using the most advanced technology and methods. The MTC will be an extension of the existing OUHSC Laboratory for Molecular Biology and Cytometry Research (LMBCR), an established and well-managed university Core laboratory. The MTC will employ personnel from within the LMBCR and INBRE Bioinformatics Core that have extensive experience in the focus areas (next gen sequencing methods, including microbiome and RNAseq, and bioinformatics) required for the Junior Investigator projects. The MTC will provide whole cell/system wide analysis of gene expression using established next generation sequencing and transcriptomics technology and appropriate bioinformatics approaches. Support for these applications by the MTC will include pre-project consultation, assistance with sample preparation and RNA isolation protocols, wet-bench sample processing, bioinformatics analysis and post project data deposition, manuscript preparation, and aid with details necessary for future grant applications.

微生物组学和转录组学核心（MTC）