Induction and Patterning of Cardiogenic Fields

心源场的感应和模式化

• 批准号: 10459497
• 负责人: Takashi Mikawa
• 金额: $ 40.38万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-28 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10459497
• 关键词: Anterior; Anterolateral; Apoptosis; Bilateral; Biological; Birds; Cell Death; Cell division; Cells; Coupled; Coupling; Cues; Destinations; Diffusion; Ectoderm; Embryo; Embryonic Development; Endoderm; Epiblast; Event; Filopodia; Gene Expression; Genetic; Germ Layers; Handedness; Heart; Hensen' s Node; Image; Individual; Lateral; Lead; Left; Mammals; Mediating; Mesoderm; Mitotic; Modeling; Molecular; Outcome; Pathway interactions; Pattern; Population; Positioning Attribute; Primitive Streaks; Process; Proliferating; Randomized; Role; Series; Signal Transduction; Site; Specific qualifier value; Testing; Time; body system; daughter cell; early onset; gastrulation; heart cell; imaging approach; intercalation; migration; novel; precursor cell; preservation; primitive cell; sensor

PROJECT SUMMARY/ABSTRACT The cardiogenic fate is established as a bilateral heart field (HF) within the anterior mesoderm through an inductive inter-germ layer signaling interaction. Many genetic pathways responsible for fate specification and differentiation of the HF have been extensively studied. However, where cells of the HF originate, how they precisely arrive at bilateral HF, or how they receive fate specification cues, remains obscured. Using live imaging of both wild type and somatic trans-genetic embryos with molecular sensors, this proposal will identify a coupling of: axis inducing signals with HF precursor positioning in the PS (Aim 1), the earlier onset of bilateral patterning (Aim 2); and a direct cell-cell contact with HF fate specification signaling transfer (Aim 3). Taken together, the proposed study will establish a novel view for the importance of coordinated positioning and interactions in the PS and at the HF forming site to define ultimate cardiogenic fate.

项目摘要/摘要 心源性命运是在前中胚层内的一个双侧心场(HF)通过一个 诱导胚层间信号相互作用。许多遗传途径负责命运的决定和 氢氟酸的鉴别已被广泛研究。然而，高频细胞的起源，它们是如何 准确地到达双边高频，或者他们如何接收命运指定线索，仍然是模糊的。使用实时成像 对于带有分子传感器的野生型和体细胞转基因胚胎，这一提议将确定一种偶联 研究对象：在PS中定位HF前体的轴诱导信号(目标1)，较早开始双侧图案化 (目标2)；以及与HF命运规范信令传送直接接触的细胞-细胞(目标3)。总而言之， 拟议的研究将为协调定位和相互作用的重要性建立一个新的观点 PS和在HF形成部位定义最终的心源性命运。