NEURAL REORGANIZATION AFTER ANTEROLATERAL CORDOTOMY

前外侧皮质切开术后的神经重组

• 批准号: 3083646
• 负责人: Elizabeth Bullitt
• 金额: $ 6.24万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3083646
• 关键词: Macaca; afferent nerve; biological models; immunochemistry; laboratory rat; nervous system regeneration; neuronal guidance; pain; spinal cord injury; spinal cord surgery

Our long term goal is to determine the nature of functional or structural reorganization that occurs in the primate spinal cord following anterolateral cordotomy, and to correlate these changes with loss and subsequent recovery of sensory function. Such delayed recovery of sensory function occurs commonly in humans following an initially successful cordotomy. Experimental work will center upon a search for change in neuronal connectivity within the spinal dorsal horn. Research on the neural reconnectivity of the primate dorsal horn has already been funded under NIH grant #NS14899. The immediate goal is to create a model of neural reorganization after cordotomy in the rat, using anatomic, physiologic, and immunocytochemical techniques applicable to the primate experiments, but without reference to behavioral changes. This model may indicate the most fruitful investigative techniques to be used in the monkey rapidly and at relatively low cost. Initial emphasis will be placed upon demonstrating differences between control and experimental animals in (1) the central arborization patterns of primary afferent fibers, and (2) the immunocytochemical staining patterns of peptides within the dorsal horn. The completeness of spinothalamic tract section and the extent of ipsilateral spinothalamic tract projections will be assessed through retrograde HRP studies. In the future, the techniques that seem most promising in the rat model will be emphasized in the primate experiments. Changes in functional or structural organization of the primate dorsal horn will be correlated with loss and recovery of sensory function after cordotomy, through behavioral testing parameters already in use in this laboratory. This project provides an ideal opportunity for the candidate to pursue her research interests, while learning anatomic, physiologic, and immunocytochemical investigational techniques.

我们的长期目标是确定功能性或结构性的本质

项目成果

Computer programs for the analysis of immunohistochemically labeled spinal cord sections.

用于分析免疫组织化学标记的脊髓切片的计算机程序。

• DOI: 10.1016/0165-0270(90)90156-a
• 发表时间: 1990
• 期刊: Journal of neuroscience methods
• 影响因子: 3
• 作者: Johnson,EM;Bullitt,E
• 通讯作者: Bullitt,E

Intraspinal course of descending serotoninergic pathways innervating the rodent dorsal horn and lamina X.

神经支配啮齿类动物背角和 X 板的下降 5-羟色胺能通路的椎内路线。

• DOI: 10.1002/cne.902860208
• 发表时间: 1989
• 期刊: The Journal of comparative neurology
• 作者: Bullitt,E;Light,AR
• 通讯作者: Light,AR

Reorganization of primary afferent nerve terminals in the spinal dorsal horn of the primate caudal to anterolateral chordotomy.

灵长类动物脊索背角初级传入神经末梢的重组，从尾部到前外侧脊索切开术。

• DOI: 10.1002/cne.902700408
• 发表时间: 1988
• 期刊: The Journal of comparative neurology
• 作者: Bullitt,E;Stofer,WD;Vierck,CJ;Perl,ER
• 通讯作者: Perl,ER