Induction and Patterning of Cardiogenic Fields

心源场的感应和模式化

• 批准号: 10668338
• 负责人: Takashi Mikawa
• 金额: $ 40.38万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-28 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10668338
• 关键词: Anterior; Anterolateral; Apoptosis; Bilateral; Biological; Birds; Cell Death; Cell division; Cells; Coupled; Coupling; Cues; Destinations; Diffusion; Ectoderm; Embryo; Embryonic Development; Endoderm; Epiblast; Event; Filopodia; Gene Expression; Genetic; Germ Layers; Handedness; Heart; Hensen' s Node; Image; Individual; Intercalated Cell; Lateral; Lead; Left; Mammals; Mediating; Mesoderm; Mitotic; Modeling; Molecular; Outcome; Pathway interactions; Pattern; Population; Positioning Attribute; Primitive Streaks; Process; Proliferating; Randomized; Role; Series; Signal Induction; Signal Transduction; Site; Specific qualifier value; Testing; Time; body system; daughter cell; early onset; gastrulation; heart cell; imaging approach; migration; novel; precursor cell; preservation; primitive cell; sensor

PROJECT SUMMARY/ABSTRACT The cardiogenic fate is established as a bilateral heart field (HF) within the anterior mesoderm through an inductive inter-germ layer signaling interaction. Many genetic pathways responsible for fate specification and differentiation of the HF have been extensively studied. However, where cells of the HF originate, how they precisely arrive at bilateral HF, or how they receive fate specification cues, remains obscured. Using live imaging of both wild type and somatic trans-genetic embryos with molecular sensors, this proposal will identify a coupling of: axis inducing signals with HF precursor positioning in the PS (Aim 1), the earlier onset of bilateral patterning (Aim 2); and a direct cell-cell contact with HF fate specification signaling transfer (Aim 3). Taken together, the proposed study will establish a novel view for the importance of coordinated positioning and interactions in the PS and at the HF forming site to define ultimate cardiogenic fate.

项目总结/摘要 心源性的命运是建立作为一个双边心脏领域（HF）内的前中胚层通过一个 诱导性胚层间信号传导相互作用。许多遗传途径负责命运的规范和 HF的分化已经被广泛研究。然而，HF的细胞起源于何处，它们如何 精确地到达双侧HF，或者他们如何接收命运指定线索，仍然模糊不清。使用实时成像 野生型和体细胞转基因胚胎与分子传感器，这一建议将确定耦合 的：轴诱导信号与HF前体定位在PS中（目的1），双侧图案化的早期发作 (Aim 2）;和与HF命运特化信号传导传递的直接细胞-细胞接触（Aim 3）。综合起来， 拟议的研究将建立一个新的观点，协调定位和相互作用的重要性， PS和HF形成部位，以确定最终的心源性结局。