Application of Automatic Extraction Platform KingFisher Apex on Viral and Bacterial Pathogen to Increase the Capacity in Vet-LIRN Sample Analysis

应用自动提取平台 KingFisher Apex 检测病毒和细菌病原体，提高 Vet-LIRN 样品分析能力

• 批准号: 10448997
• 负责人: Lifang Yan
• 金额: $ 6万
• 依托单位: MISSISSIPPI STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-06 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10448997
• 关键词: Application; Automatic; Extraction; Platform; KingFisher

Project Summary The Mississippi Veterinary Research and Diagnostic Laboratory (MVRDL) is a full service, AAVLD accredited, all species, central reference laboratory. The molecular section personnel consists of: a section supervisor with a B.S., M.S, and Ph.D. in veterinary sciences with more than seven-year experience in molecular biology and virology in the veterinary diagnostic laboratory, one technician with twelve years working experience in the molecular diagnostic laboratory and thirteen years in related medical research experience, and one technician with more than seven years working experience in molecular diagnostics and poultry virology and over 6 years working experience in medical research. The section analyses samples from approximately 2000 accessions per year from which over 5000 PCR procedures (conventional PCR, real time PCR, sequencing) are completed. MVRDL is a member of the VET-LIRN, one of the veterinary diagnostic labs that participated in SARS-CoV2 testing for both human and animals, which helps the university test students and staff and address the concerns of pet or livestock owners. We also participated in an interlaboratory group project - evaluation of an automated DNA extraction for food-borne pathogen isolates and development of a harmonized method for sequencing Listeria monocytogenes and E. coli on iSeq100. Automatic extraction equipment is widely used for all routine diagnostics, SARS-CoV2 testing, whole genome sequencing and extraction comparison projects. Currently we only have one KingFisher Flex extraction system with five years of usage. However, different pathogens or different protocols require use of different programs on the equipment. In order to maintain the short turn-around time and keep efficiency of diagnostic tests, we would like to get support to purchase a new automatic extraction system, KingFisher Apex benchtop sample preparation to increase the testing capacity and also serve as a backup for current KingFisher Flex. The KingFisher Apex benchtop is a newer model of KingFisher Flex. MVRDL has the capacity and is willing to offer its’ service to increase the capacity of the VPO in handling increased sample workflow during emergencies. MVRDL is committed to performing these services in an efficient, accurate and timely fashion and to reporting results to the required agency.

项目摘要 密西西比兽医研究和诊断实验室（MVRDL）是一个全方位服务，AAVLD认证， 所有种属，中心参考实验室。分子科人员包括： 学士学位硕士和博士在兽医科学领域拥有七年以上的分子生物学经验， 兽医诊断实验室的病毒学，一名技术员，具有十二年的工作经验， 分子诊断实验室和十三年相关医学研究经验，以及一名技术人员 在分子诊断和家禽病毒学方面有七年以上的工作经验， 医学研究工作经验。该部分分析了大约2000份加入的样品 每年有超过5000个PCR程序（常规PCR，真实的时间PCR，测序）， 完成 MVRDL是VET-LIRN的成员，VET-LIRN是参与SARS-CoV 2的兽医诊断实验室之一 为人类和动物进行测试，这有助于大学测试学生和工作人员，并解决 宠物或牲畜主人的担忧。我们还参加了一个实验室间小组项目-评估一个 食源性病原体分离株的自动DNA提取和开发统一方法 测序单核细胞增生李斯特菌和E.大肠杆菌在iSeq 100上的表达。 自动提取设备广泛用于所有常规诊断、SARS-CoV 2检测、全基因组检测、 测序和提取比较项目。目前我们只有一个KingFisher Flex提取系统 五年的使用。然而，不同的病原体或不同的方案需要使用不同的程序 在设备上。为了保持较短的周转时间和保持诊断测试的效率，我们 我想获得支持，购买一个新的自动提取系统，KingFisher Apex台式样品 这是一个新的测试平台，可以增加测试容量，也可以作为当前KingFisher Flex的备份。的 KingFisher Apex benchtop是KingFisher Flex的新型号。 MVRDL有能力并愿意提供服务，以增加VPO的处理能力 增加紧急情况下的样本工作流程。MVRDL致力于以一种 以高效、准确和及时的方式，并向所需机构报告结果。