Application of gel imaging system to increase efficiency when determine presence of PCR amplicon of bacterial pathogens in Vet-LIRN sample analysis

项目摘要 密西西比州兽医研究和诊断实验室(MVRDL)是一项全方位的服务，AAVLD认证， 所有物种，中心参考实验室。分子科员由1名科长和1名 拥有兽医科学学士、硕士和博士学位，具有六年以上分子生物学和 兽医诊断实验室病毒学，一名有11年分子工作经验的技术员 诊断实验室和12年的医学相关研究经验，以及一名技术人员具有以上 6年以上分子诊断和家禽病毒学工作经验，5年以上工作经验 在医学研究方面。该部门分析了每年约2000份材料的样本，其中 已完成5000多个聚合酶链式反应程序(常规聚合酶链式反应、实时聚合酶链式反应、测序)。 MVRDL是VET-LIRN的成员，我们是参与VET-LIRN抗菌剂的实验室之一 阻力试点项目。常规的聚合酶链式反应被用来验证细菌分离。我们拥有的凝胶成像系统 MVRDL已有15年以上的历史，安装这台机器的计算机是Windows 2002。因此， 这台机器几乎不能使用了。我们希望获得支持购买QIAxcel，以取代我们目前的凝胶图像 系统。QIAxcel先进的自动化电泳系统也能够评估NEXT的质量控制 生成测序库，这将进一步增强我们的结果，并允许我们使用下一代 其他项目中的测序(NGS)。 所提出的QIAxcel先进的自动电泳仪不仅是为了检测 常规的聚合酶链式反应和产生高质量的凝胶图像，但要检查NGS文库的质量控制。 MVRDL有能力并愿意提供自己的服务，以提高VPO的处理能力 增加了紧急情况下的样本工作流程。MVRDL致力于在一个 高效率、准确和及时，并向所需机构报告结果。

Project Summary The Mississippi Veterinary Research and Diagnostic Laboratory (MVRDL) is a full service, AAVLD accredited, all species, central reference laboratory. The molecular section personnel consists of: a section supervisor with a B.S., M.S, and Ph.D in veterinary sciences with more than six year experience in molecular biology and virology of veterinary diagnostic laboratory, one technician with 11 years working experience in molecular diagnostic laboratory and 12 years in medical related research experience, and one technician with more than 6 years working experience in molecular diagnostic and poultry virology and over 5 years working experience in medical research. The section analyses samples from approximately 2000 accessions per year from which over 5000 PCR procedures (conventional PCR, real time PCR, sequencing) are completed. MVRDL is a member of the VET-LIRN, and we are one of the labs involved in the VET-LIRN antimicrobial resistance pilot project. Conventional PCR is used to verify bacteria isolates. The gel image system we have in MVRDL is more than 15 years old, and the computer with the machine is windows 2002. Therefore, the machine is almost out of service. We would like to get support to buy QIAxcel to replace our current gel image system. The QIAxcel advanced automated electrophoresis system is also able to assess QC of next generation sequencing libraries which would further enhance our results, and allow us to use next generation sequencing (NGS) in other projects. The proposed QIAxcel advanced automated electrophoresis system is not only to check PCR amplicons of conventional PCR test and generated high quality gel image, but to check QC of NGS library. MVRDL has the capacity and is willing to offer its' service to increase the capacity of the VPO in handling increased sample workflow during emergencies. MVRDL is committed to performing these services in an efficient, accurate and timely fashion and to reporting results to the required agency.