Application of gel imaging system to increase efficiency when determine presence of PCR amplicon of bacterial pathogens in Vet-LIRN sample analysis

应用凝胶成像系统提高 Vet-LIRN 样品分析中确定细菌病原体 PCR 扩增子的效率

• 批准号: 9916447
• 负责人: Lifang Yan
• 金额: $ 3.43万
• 依托单位: MISSISSIPPI STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-05 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9916447
• 关键词: Application; gel; imaging; system; increase

Project Summary The Mississippi Veterinary Research and Diagnostic Laboratory (MVRDL) is a full service, AAVLD accredited, all species, central reference laboratory. The molecular section personnel consists of: a section supervisor with a B.S., M.S, and Ph.D in veterinary sciences with more than six year experience in molecular biology and virology of veterinary diagnostic laboratory, one technician with 11 years working experience in molecular diagnostic laboratory and 12 years in medical related research experience, and one technician with more than 6 years working experience in molecular diagnostic and poultry virology and over 5 years working experience in medical research. The section analyses samples from approximately 2000 accessions per year from which over 5000 PCR procedures (conventional PCR, real time PCR, sequencing) are completed. MVRDL is a member of the VET-LIRN, and we are one of the labs involved in the VET-LIRN antimicrobial resistance pilot project. Conventional PCR is used to verify bacteria isolates. The gel image system we have in MVRDL is more than 15 years old, and the computer with the machine is windows 2002. Therefore, the machine is almost out of service. We would like to get support to buy QIAxcel to replace our current gel image system. The QIAxcel advanced automated electrophoresis system is also able to assess QC of next generation sequencing libraries which would further enhance our results, and allow us to use next generation sequencing (NGS) in other projects. The proposed QIAxcel advanced automated electrophoresis system is not only to check PCR amplicons of conventional PCR test and generated high quality gel image, but to check QC of NGS library. MVRDL has the capacity and is willing to offer its' service to increase the capacity of the VPO in handling increased sample workflow during emergencies. MVRDL is committed to performing these services in an efficient, accurate and timely fashion and to reporting results to the required agency.

项目摘要 密西西比兽医研究和诊断实验室（MVRDL）是一个全方位服务，AAVLD认证， 所有种属，中心参考实验室。分子科人员包括： 学士学位兽医科学硕士和博士学位，在分子生物学方面有六年以上的经验， 兽医诊断实验室病毒学，1名技术员，具有11年分子生物学工作经验 诊断实验室和12年的医学相关研究经验，以及一名技术员， 6年分子诊断和家禽病毒学工作经验，5年以上工作经验 在医学研究中。该部分分析了每年约2000份材料的样本，其中 完成超过5000个PCR程序（常规PCR、真实的时间PCR、测序）。 MVRDL是VET-LIRN的成员，我们是参与VET-LIRN抗菌剂的实验室之一 抵抗试点项目。常规PCR用于验证细菌分离株。我们的凝胶成像系统 MVRDL已经有15年多的历史了，机器配备的电脑是windows 2002。因此 机器几乎停止使用。我们希望获得支持，购买QIAxcel，以取代我们目前的凝胶图像 系统QIAxcel先进的自动电泳系统还能够评估下一个样品的质量控制。 这将进一步增强我们的结果，并允许我们使用下一代测序文库， 其他项目中的测序（NGS）。 所提出的QIAxcel高级自动电泳系统不仅可以检查 常规PCR测试并产生高质量凝胶图像，但检查NGS文库的QC。 MVRDL有能力并愿意提供服务，以增加VPO的处理能力 增加紧急情况下的样本工作流程。MVRDL致力于以一种 以高效、准确和及时的方式，并向所需机构报告结果。