Enhancing food contaminant surveillance capacity using ultra-performance liquid chromatography with UV/fluorescence detection

项目总结/摘要： 密苏里州（MU）兽医医学诊断实验室（VMDL）的大学是唯一的全方位服务 美国兽医实验室协会认可的兽医诊断实验室 诊断师（AAVLD）在密苏里州。三十多年来，MU VMDL的诊断毒理学 和分析化学组（DTACS）提供了关键的分析支持，以检测各种 潜在的有毒物质，特别是黄曲霉毒素和麦角碱，污染牲畜饲料和宠物 食品因为这些和其他食物污染物不仅会伤害动物， 供人类食用的食品中存在污染物残留， Vet-LIRN的监测活动必须敏感、准确、快速和具有成本效益。VMDL DTACS是 充分致力于扩大其分析能力，以更好地支持全州，区域和国家的诊断 需要，以及增加参与Vet-LIRN的监测工作，旨在减少 动物和人类的食源性疾病。DTACS分析化学家杜祥伟博士和Tim Evans博士， DTACS负责人将担任共同主要研究者。杜博士在与Vet-LIRN和 诊断方法的开发和优化。在帮助FDA确定 最近的宠物食品污染涉及黄曲霉毒素，杜和埃文斯的结论是，高性能液体 DTACS使用的高效液相色谱（HPLC）分离方法不再能提供最佳和 对诊断样本中常见食品污染物的低浓度进行一致测量， 无法充分满足Vet-LIRN在牲畜广泛传播的情况下的大量快速反应需求 饲料或宠物食品污染。鉴于许多公立赠地大学面临的财政限制， 包括MU在内，MU VMDL DTACS更新其分析能力的工作必须以 战略性和渐进性的方式。用超高效液相色谱仪取代HPLC仪器 色谱法（UPLC或UHPLC）将提供四倍的样品处理速度和至少五倍的 污染物检测分辨率高于HPLC。这一升级将立即增强分析能力， DTACS诊断服务的功能，为未来分析能力的扩展提供平台， 允许更多的人参与Vet-LIRN的食品污染物监测工作。具体目标是 赠款建议是1）购买一个新的UPLC与紫外和荧光检测器; 2）优化方法， UPLC用于测量各种不同基质中的真菌毒素，特别是黄曲霉毒素和麦角碱生物碱; 和3）开发使用UPLC测量大麻素和其他分析物的新方法，特别是那些 Vet-LIRN的兴趣，在选定的矩阵。这些具体目标的实现将充分扩大 DTACS的分析能力，以促进改善诊断服务， Vet-LIRN的使命是提高人类和动物食品安全。

PROJECT SUMMARY/ABSTRACT: The University of Missouri (MU) Veterinary Medical Diagnostic Laboratory (VMDL) is the only full-service veterinary diagnostic laboratory accredited by the American Association of Veterinary Laboratory Diagnosticians (AAVLD) in the state of Missouri. For over thirty years, the MU VMDL’s Diagnostic Toxicology and Analytical Chemistry Section (DTACS) has provided critical analytical support to detect a variety of potential toxicants, especially aflatoxins and ergopeptine alkaloids that contaminate livestock feeds and pet foods. Because these and other food contaminants can not only harm animals but can also result in the presence of contaminant residues in foodstuffs intended for human consumption, the food contaminant surveillance activities of Vet-LIRN must be sensitive, accurate, rapid, and cost-effective. The VMDL DTACS is fully committed to expand its analytical capacity to better support statewide, regional, and national diagnostic needs, as well as increase participation in Vet-LIRN’s surveillance efforts intended to reduce the incidence of foodborne illness in animals and humans. Dr. Xiangwei Du, DTACS Analytical Chemist, and Dr. Tim Evans, DTACS Head, will serve as Co-Principal Investigators. Dr. Du has expertise in working with Vet-LIRN and the development and optimization of diagnostic methods. While helping the FDA to determine the scope of a recent pet food contamination involving aflatoxins, Du and Evans concluded that the high-performance liquid chromatographic (HPLC) separation methods used by the DTACS could no longer provide optimal and consistent measurement of very low concentrations of common food contaminants in diagnostic samples and could not adequately meet Vet-LIRN’s high-volume, rapid-response needs in instances of widespread livestock feed or pet food contamination. Given the financial constraints facing many public, land-grant universities, including MU, the efforts of the MU VMDL DTACS to update its analytical capabilities must proceed in a strategic and stepwise manner. The replacement of HPLC instrument capabilities with ultra-performance liquid chromatography (UPLC or UHPLC), will provide four times faster sample processing and at least five times greater contaminant detection resolution than HPLC. This upgrade will immediately enhance the analytical capabilities of the DTACS diagnostic services, provide a platform for future analytical capacity expansion, and allow increased participation in Vet-LIRN’s food contaminant surveillance efforts. The Specific Aims of this grant proposal are 1) purchase a new UPLC with UV and fluorescence detectors; 2) optimize methods using UPLC to measure mycotoxins, especially aflatoxins and ergopeptine alkaloids, in various different matrices; and 3) develop new methods using UPLC to measure cannabinoids and other analytes, particularly those of interest to Vet-LIRN, in selected matrices. Accomplishment of these Specific Aims will sufficiently expand the analytical capacity of the DTACS to facilitate both improved diagnostic services and more meaningful contributions to Vet-LIRN’s mission to enhance human and animal food safety.