The Role of Lipin1 in Myofiber Stability and Integrity

Lipin1 在肌纤维稳定性和完整性中的作用

• 批准号: 10455623
• 负责人: Hongmei Ren
• 金额: $ 37.22万
• 依托单位: WRIGHT STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-22 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10455623
• 关键词: Activities of Daily Living; Adult; Affect; Age; Anabolism; Attenuated; Cell Death; Cell membrane; Cessation of life; ChIP-seq; Clinical; Data; Diglycerides; Disease; Disease Progression; Disease model; Dose; Duchenne muscular dystrophy; Dystrophin; Enzymes; Gene Delivery; Gene Expression; Gene Expression Profile; Gene Transduction Agent; Genes; Genetic Transcription; Glycerophospholipids; Goals; Individual; Intramuscular; Lecithin; Lipids; Live Birth; Mediating; Membrane; Methods; Mission; Molecular; Morphology; Mus; Muscle; Muscle Cells; Muscle Contraction; Muscle Fibers; Muscle Weakness; Muscle function; Muscular Atrophy; Muscular Dystrophies; Myopathy; Pathologic; Pathology; Patients; Phase; Phenotype; Phosphatidate Phosphatase; Phosphatidic Acid; Phospholipids; Phosphoric Monoester Hydrolases; Play; Predisposition; Protein Isoforms; Public Health; Publications; Regenerative capacity; Regimen; Research; Resistance; Rhabdomyolysis; Role; Safety; Signal Transduction; Skeletal Muscle; Specificity; Stress; Structural Genes; Teenagers; Testing; Therapeutic; Transcription Coactivator; Transcriptional Regulation; Transgenic Mice; Transgenic Organisms; Treatment Efficacy; United States National Institutes of Health; Up-Regulation; Wheelchairs; Work; age-related muscle loss; aged; base; disability; effective therapy; exhaust; gene therapy; improved; intravenous administration; lipine; mRNA Expression; mdx mouse; micro-dystrophin; mortality; motor deficit; mouse model; muscle degeneration; muscle regeneration; mutant; myocyte-specific enhancer-binding-factor 2C; new therapeutic target; novel; overexpression; prevent; protective effect; protein expression; tool; transduction efficiency; young adult

PROJECT SUMMARY Duchenne muscular dystrophy (DMD) is a progressive and devastating muscle disease, resulting from the absence of dystrophin. This leads to cell membrane instability, susceptibility to contraction-induced muscle damage, muscle cell death, and disability and early death of patients. Currently, there is no cure for DMD. Lipin1 is a phosphatidic acid (PA) phosphatase (PAP) enzyme that catalyzes diacylglycerol (DAG) biosynthesis. It is also the predominant lipin isoform in skeletal muscles. In addition to its enzymatic activity, lipin1 can regulate transcription. A recent publication from this lab identified a previously unknown role of lipin1 in promoting myocyte enhancer factor 2c (MEF2c) transcriptional activity via DAG signaling in skeletal muscle. Notably, our findings showed that loss of lipin1 leads to myopathy. Preliminary data also showed that lipin1 expression was dramatically reduced in the skeletal muscles of DMD patients and mdx (DMDmdx) mice, a mouse model for DMD. Strikingly, further data show that increasing lipin1 levels in mdx mice lessened muscle fiber degeneration, strengthened membrane integrity, and led to impressive gains in strength. Based on these novel findings, the central hypothesis of this proposal is that lipin1 overexpression ameliorates the dystrophic phenotype in DMD through its PAP activity and its ability to regulate transcription. This hypothesis will be tested via the following Specific Aims: 1) Assess the pathological and functional benefit of lipin1 overexpression in mdx:lipin1 transgenic mice in early and late stages of the disease; 2) Determine the therapeutic efficacy of AAV9-mediated systemic lipin1 gene delivery; and 3) Test the hypothesis that lipin1 regulates muscle membrane integrity through the transcriptional regulation of structural genes and through PAP activity. NIH Mission Relevance: DMD affects 15.9-19.5 individuals per 100,000 live births. Specifically, DMD patients often manifest disability around age 3-5, and many require a wheelchair before age 12. Progressive muscle weakness often results in early mortality around age 30. This research seeks to elucidate the role of lipin1 as a novel regulator to prevent dystrophic pathologies, and will determine whether lipin1 can serve as a target to re- establish membrane stability and restore muscle function. Such information is expected to have a transformative impact on the treatment of DMD, as well as open new avenues for the treatment of several clinically related conditions, such as rhabdomyolysis, age-related muscle loss, and other muscular dystrophies which share common features associated with decreased lipin1 expression.

项目总结