Ethnopharmacology of plants used by Haitians in New York City forwomen’s health

纽约市海地人使用的植物对女性健康的民族药理学

• 批准号: 10640068
• 负责人: Ella Vardeman
• 金额: $ 4.35万
• 依托单位: CUNY GRADUATE SCH AND UNIV CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10640068
• 关键词: Address; Anti-Bacterial Agents; Antimicrobial Effect; Back; Bacteria; Bixa; Botanica; Botanicals; COVID-19 pandemic; Caribbean region; Chemicals; Cities; Coculture Techniques; Collaborations; Commerce; Communities; Country; Cross-Cultural Comparison; Data; Deposition; Disease; Effectiveness; Environment; Ethnobotany; Ethnopharmacology; Evaluation; Feedback; Fractionation; Future; Gardnerella vaginalis; Growth; Gynecologic; Haitian; Health; Home; Immigrant; In Vitro; Infection; International; Interview; Investigation; Jatropha; Knowledge; Laboratories; Laboratory Study; Lactobacillus; Literature; Marketing; Measures; Medical; Medical Students; Medicinal Plants; Medicine; Methodology; Methods; Monograph; Natural Products; New York; New York City; Pathogenicity; Perception; Persons; Phytochemical; Plant Extracts; Plant Leaves; Plants; Population; Preparation; Prickly Poppy; Property; Public Health; Reporting; Research; Self Medication; Sexually Transmitted Diseases; Specimen; Statistical Data Interpretation; Surveys; Taxonomy; Testing; Tissues; Traditional Medicine; United Nations; Vagina; Validation; Variant; Woman; Women' s Health; World Health Organization; antimicrobial; chemical fingerprinting; cost; cultural competence; ethnomedicine; healing; improved; insight; member; metabolomics; microbiota; outreach; pathogenic bacteria; pathogenic microbe; programs; vaginal infection; vaginal microbiota

Proposal Abstract Approximately 55% of the world’s population lives in cities, and the United Nations estimates that this number will rise to 68% by 2050. Urban ethnobotany, the study of people’s traditional knowledge and use of plants in cities, is increasingly important as people adapt their cultural traditions to changing environments. In New York City (NYC), there have been several urban ethnobotany investigations with Caribbean immigrant groups. However, there has yet to be an ethnobotanical survey that documents and botanically authenticates the medicinal plants used in the Haitian community. Previous research in collaboration with women from the Caribbean in NYC has identified plant species sold in Caribbean shops that have documented use for the treatment of vaginal infections, including sexually transmitted infections and intravaginal cleansing, grouped here as gynecological conditions. Several popular Caribbean plants, such as Jatropha curcas, Argemone mexicana, and Bixa orellana, have recorded medicinal uses in Haitian ethnomedicine as well. These species, while well- studied for their antibacterial properties, have not yet been analyzed for their effects on pathogenic bacteria in the vagina or the protective vaginal microbiota. Ideally, plants used to treat these conditions would have bioactivity against harmful bacteria with minimal disruption to the beneficial vaginal microflora. Natural product research that considers interactions with pathogenic and beneficial bacteria, as well as the compounds responsible, is on the forefront of addressing efficacy of medicinal plants for women’s health. I hypothesize that traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella vaginalis) and beneficial (Lactobacillus) vaginal bacterial due to differences in specialized metabolites. Furthermore, I hypothesize that there will be similarities in plants and women’s health practices between the Haitian and other Caribbean communities in NYC, due to the shared flora in the Caribbean. The selected plant species will be analyzed for their effects on disease-causing bacteria, Gardnerella vaginalis as well as on prominent beneficial Lactobacillus species found in the vagina. Plant extracts will also be compared to determine variation in the composition of bioactive phytochemicals across preparations of different tissues, using LC-MS- qToF and subsequent multivariate statistical analysis. An ethnobotanical survey with the Haitian community will be used to further identify additional plant species used to treat gynecological conditions by Caribbean women for continued laboratory studies. This data will enable cross-cultural comparisons with other Caribbean communities in NYC, and will also assess how the COVID-19 pandemic has influenced NYC Haitian women in their use of medicinal plants for women’s health. Antibacterial natural products from plants that leave the vaginal microflora intact may provide low-cost and culturally-appropriate solutions for ongoing treatment of gynecological conditions.

提案摘要 世界上大约55%的人口生活在城市，联合国估计， 到2050年将上升到68%。城市民族植物学，研究人们的传统知识和植物的使用， 随着人们使自己的文化传统适应不断变化的环境，城市的文化越来越重要。在纽约 城市（纽约市），有几个城市民族植物学调查与加勒比移民群体。 然而，还没有一个民族植物学调查，文件和植物学认证的， 海地社区使用的药用植物。以前的研究与妇女合作， 纽约市的加勒比地区已经确定了在加勒比商店出售的植物物种，这些植物物种已经记录了 治疗阴道感染，包括性传播感染和阴道内清洗，分组如下 妇科疾病几种流行的加勒比植物，如麻风树，墨西哥Argemone， 和Bixa orellana，也记录了海地民族医学的药用价值。这些物种，虽然很好- 研究了它们的抗菌性能，尚未分析它们对病原菌的影响， 阴道或保护性阴道微生物群。理想情况下，用于治疗这些疾病的植物应该 对有害细菌的生物活性，对有益的阴道微生物区系的破坏最小。天然产物 研究，考虑与致病菌和有益细菌的相互作用，以及化合物 负责的，是在解决妇女健康的药用植物的功效的前沿。我假设 所鉴定的物种的传统制剂在抑制病原体（加德纳菌）生长方面会有所不同 阴道杆菌）和有益的（乳酸杆菌）阴道细菌，这是由于专门代谢物的差异。 此外，我假设，将有相似之处，植物和妇女的健康做法之间的 海地人和纽约市的其他加勒比社区，因为加勒比地区共有植物群。所选植物 物种将分析其对致病细菌，加德纳菌以及 阴道中发现的显著有益的乳酸菌物种。植物提取物也将进行比较，以确定 使用LC-MS，在不同组织的制备物中生物活性植物化学物质的组成的变化， qToF和随后的多变量统计分析。对海地社区的民族植物学调查将 用于进一步确定加勒比妇女用于治疗妇科疾病的其他植物物种 继续进行实验室研究。这些数据将有助于与加勒比其他国家进行跨文化比较。 纽约市的海地社区，并将评估COVID-19大流行如何影响纽约市的海地妇女， 他们使用药用植物来保护女性健康抗菌天然产品从植物离开阴道 完整的微生物群落可以为妇科疾病的持续治疗提供低成本和文化上合适的解决方案。 条件