Ethnopharmacology of plants used by Haitians in New York City forwomen’s health

纽约市海地人使用的植物对女性健康的民族药理学

• 批准号: 10640068
• 负责人: Ella Vardeman
• 金额: $ 4.35万
• 依托单位: CUNY GRADUATE SCH AND UNIV CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10640068
• 关键词: Address; Anti-Bacterial Agents; Antimicrobial Effect; Back; Bacteria; Bixa; Botanica; Botanicals; COVID-19 pandemic; Caribbean region; Chemicals; Cities; Coculture Techniques; Collaborations; Commerce; Communities; Country; Cross-Cultural Comparison; Data; Deposition; Disease; Effectiveness; Environment; Ethnobotany; Ethnopharmacology; Evaluation; Feedback; Fractionation; Future; Gardnerella vaginalis; Growth; Gynecologic; Haitian; Health; Home; Immigrant; In Vitro; Infection; International; Interview; Investigation; Jatropha; Knowledge; Laboratories; Laboratory Study; Lactobacillus; Literature; Marketing; Measures; Medical; Medical Students; Medicinal Plants; Medicine; Methodology; Methods; Monograph; Natural Products; New York; New York City; Pathogenicity; Perception; Persons; Phytochemical; Plant Extracts; Plant Leaves; Plants; Population; Preparation; Prickly Poppy; Property; Public Health; Reporting; Research; Self Medication; Sexually Transmitted Diseases; Specimen; Statistical Data Interpretation; Surveys; Taxonomy; Testing; Tissues; Traditional Medicine; United Nations; Vagina; Validation; Variant; Woman; Women' s Health; World Health Organization; antimicrobial; chemical fingerprinting; cost; cultural competence; ethnomedicine; healing; improved; insight; member; metabolomics; microbiota; outreach; pathogenic bacteria; pathogenic microbe; programs; vaginal infection; vaginal microbiota

Proposal Abstract Approximately 55% of the world’s population lives in cities, and the United Nations estimates that this number will rise to 68% by 2050. Urban ethnobotany, the study of people’s traditional knowledge and use of plants in cities, is increasingly important as people adapt their cultural traditions to changing environments. In New York City (NYC), there have been several urban ethnobotany investigations with Caribbean immigrant groups. However, there has yet to be an ethnobotanical survey that documents and botanically authenticates the medicinal plants used in the Haitian community. Previous research in collaboration with women from the Caribbean in NYC has identified plant species sold in Caribbean shops that have documented use for the treatment of vaginal infections, including sexually transmitted infections and intravaginal cleansing, grouped here as gynecological conditions. Several popular Caribbean plants, such as Jatropha curcas, Argemone mexicana, and Bixa orellana, have recorded medicinal uses in Haitian ethnomedicine as well. These species, while well- studied for their antibacterial properties, have not yet been analyzed for their effects on pathogenic bacteria in the vagina or the protective vaginal microbiota. Ideally, plants used to treat these conditions would have bioactivity against harmful bacteria with minimal disruption to the beneficial vaginal microflora. Natural product research that considers interactions with pathogenic and beneficial bacteria, as well as the compounds responsible, is on the forefront of addressing efficacy of medicinal plants for women’s health. I hypothesize that traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella vaginalis) and beneficial (Lactobacillus) vaginal bacterial due to differences in specialized metabolites. Furthermore, I hypothesize that there will be similarities in plants and women’s health practices between the Haitian and other Caribbean communities in NYC, due to the shared flora in the Caribbean. The selected plant species will be analyzed for their effects on disease-causing bacteria, Gardnerella vaginalis as well as on prominent beneficial Lactobacillus species found in the vagina. Plant extracts will also be compared to determine variation in the composition of bioactive phytochemicals across preparations of different tissues, using LC-MS- qToF and subsequent multivariate statistical analysis. An ethnobotanical survey with the Haitian community will be used to further identify additional plant species used to treat gynecological conditions by Caribbean women for continued laboratory studies. This data will enable cross-cultural comparisons with other Caribbean communities in NYC, and will also assess how the COVID-19 pandemic has influenced NYC Haitian women in their use of medicinal plants for women’s health. Antibacterial natural products from plants that leave the vaginal microflora intact may provide low-cost and culturally-appropriate solutions for ongoing treatment of gynecological conditions.

建议书摘要 世界上大约55%的人口生活在城市，联合国估计这一数字 到2050年将上升到68%。城市民族植物学，研究人们的传统知识和对城市植物的利用 随着人们使他们的文化传统适应不断变化的环境，城市变得越来越重要。在纽约 在纽约市，对加勒比移民群体进行了几次城市民族植物学调查。 然而，目前还没有一项民族植物学调查来记录和鉴定 海地社区使用的药用植物。之前与来自美国的女性合作的研究 纽约市的加勒比海已经确定了加勒比海商店出售的植物物种，这些植物已经记录了这种植物的用途 阴道感染的治疗，包括性传播感染和阴道内清洁，在这里归类 作为妇科疾病。几种受欢迎的加勒比海植物，如麻疯树、墨西哥阿吉莫纳、 和Bixa Orellana，也记录了海地民族医学的药用用途。这些物种，虽然- 研究了它们的抗菌性能，还没有分析它们对病原菌的影响 阴道或保护性的阴道微生物区系。理想情况下，用来治疗这些情况的植物应该有 对有害细菌的生物活性，对有益的阴道微生物区系的破坏最小。天然产物 考虑与致病细菌和有益细菌以及化合物的相互作用的研究 负责任的，在解决药用植物对妇女健康的功效方面走在了前列。我假设 已鉴定物种的传统制剂在抑制致病菌(Gardnerella)的生长方面会有所不同 阴道细菌)和有益的(乳杆菌)阴道细菌，因为特殊代谢物的不同。 此外，我假设在植物和妇女的健康实践方面会有相似之处 纽约的海地和其他加勒比社区，由于加勒比海共有的植物区系。选定的植物 将分析物种对致病细菌、阴道加德纳氏菌以及 在阴道中发现的突出的有益乳酸菌种类。植物提取物也将进行比较，以确定 用LC-MS-MS研究不同组织制剂中生物活性植物化学成分的变化 QToF和随后的多变量统计分析。与海地社区进行的民族植物学调查将 用于进一步确定加勒比妇女用于治疗妇科疾病的其他植物物种 继续进行实验室研究。这一数据将有助于与其他加勒比地区进行跨文化比较。 ，还将评估新冠肺炎疫情如何影响纽约市的海地妇女 他们使用药用植物促进妇女健康。从离开阴道的植物中提取的抗菌天然产品 完整的微生物区系可能为正在进行的妇科治疗提供低成本和文化上合适的解决方案 条件。