Ethnopharmacology of plants used by Haitians in New York City forwomen’s health

纽约市海地人使用的植物对女性健康的民族药理学

• 批准号: 10388470
• 负责人: Ella Vardeman
• 金额: $ 4.26万
• 依托单位: CUNY GRADUATE SCH AND UNIV CTR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10388470
• 关键词: Address; Anti-Bacterial Agents; Antimicrobial Effect; Back; Bacteria; Bixa; Botanica; Botanicals; COVID-19 pandemic; Caribbean region; Chemicals; Cities; Coculture Techniques; Collaborations; Commerce; Communities; Country; Cross-Cultural Comparison; Data; Deposition; Disease; Effectiveness; Environment; Ethnobotany; Ethnopharmacology; Evaluation; Feedback; Fractionation; Future; Gardenal; Gardnerella vaginalis; Growth; Haitian; Health; Home; Immigrant; In Vitro; Infection; International; Interview; Investigation; Jatropha; Knowledge; Laboratories; Laboratory Study; Lactobacillus; Literature; Measures; Medical; Medical Students; Medicinal Plants; Methodology; Methods; Monograph; Natural Products; New York; New York City; Pathogenicity; Perception; Persons; Phytochemical; Plant Extracts; Plant Leaves; Plant Preparations; Plant Roots; Plants; Population; Preparation; Prickly Poppy; Property; Public Health; Reporting; Research; Seeds; Self Medication; Sexually Transmitted Diseases; Specimen; Statistical Data Interpretation; Surveys; Taxonomy; Testing; Tissues; Traditional Medicine; United Nations; Vagina; Validation; Variant; Woman; Women' s Health; World Health Organization; antimicrobial; chemical fingerprinting; cost; cultural competence; ethnomedicine; healing; improved; insight; member; metabolomics; microbiota; outreach; pathogenic bacteria; pathogenic microbe; programs; vaginal infection; vaginal microbiota; voucher

Proposal Abstract Approximately 55% of the world’s population lives in cities, and the United Nations estimates that this number will rise to 68% by 2050. Urban ethnobotany, the study of people’s traditional knowledge and use of plants in cities, is increasingly important as people adapt their cultural traditions to changing environments. In New York City (NYC), there have been several urban ethnobotany investigations with Caribbean immigrant groups. However, there has yet to be an ethnobotanical survey that documents and botanically authenticates the medicinal plants used in the Haitian community. Previous research in collaboration with women from the Caribbean in NYC has identified plant species sold in Caribbean shops that have documented use for the treatment of vaginal infections, including sexually transmitted infections and intravaginal cleansing, grouped here as gynecological conditions. Several popular Caribbean plants, such as Jatropha curcas, Argemone mexicana, and Bixa orellana, have recorded medicinal uses in Haitian ethnomedicine as well. These species, while well- studied for their antibacterial properties, have not yet been analyzed for their effects on pathogenic bacteria in the vagina or the protective vaginal microbiota. Ideally, plants used to treat these conditions would have bioactivity against harmful bacteria with minimal disruption to the beneficial vaginal microflora. Natural product research that considers interactions with pathogenic and beneficial bacteria, as well as the compounds responsible, is on the forefront of addressing efficacy of medicinal plants for women’s health. I hypothesize that traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella vaginalis) and beneficial (Lactobacillus) vaginal bacterial due to differences in specialized metabolites. Furthermore, I hypothesize that there will be similarities in plants and women’s health practices between the Haitian and other Caribbean communities in NYC, due to the shared flora in the Caribbean. The selected plant species will be analyzed for their effects on disease-causing bacteria, Gardnerella vaginalis as well as on prominent beneficial Lactobacillus species found in the vagina. Plant extracts will also be compared to determine variation in the composition of bioactive phytochemicals across preparations of different tissues, using LC-MS- qToF and subsequent multivariate statistical analysis. An ethnobotanical survey with the Haitian community will be used to further identify additional plant species used to treat gynecological conditions by Caribbean women for continued laboratory studies. This data will enable cross-cultural comparisons with other Caribbean communities in NYC, and will also assess how the COVID-19 pandemic has influenced NYC Haitian women in their use of medicinal plants for women’s health. Antibacterial natural products from plants that leave the vaginal microflora intact may provide low-cost and culturally-appropriate solutions for ongoing treatment of gynecological conditions.

提案摘要 世界上大约 55% 的人口居住在城市，联合国估计这个数字 到 2050 年将上升到 68%。 城市民族植物学，研究人们在城市中的传统知识和植物使用 随着人们使自己的文化传统适应不断变化的环境，城市变得越来越重要。在纽约 纽约市，已经对加勒比移民群体进行了几项城市民族植物学调查。 然而，目前还没有一项民族植物学调查来记录和植物学上验证 海地社区使用的药用植物。先前与女性合作的研究 纽约市的加勒比地区已经确定了在加勒比地区商店出售的植物物种，这些植物物种已记录用于 阴道感染的治疗，包括性传播感染和阴道内清洁，在此分组 如妇科病症。几种流行的加勒比植物，如麻风树、墨西哥银杏、 和 Bixa orellana 在海地民族医学中也有药用用途。这些物种虽然很好 研究了其抗菌特性，但尚未分析其对病原菌的影响 阴道或保护性阴道微生物群。理想情况下，用于治疗这些疾病的植物应该具有 针对有害细菌的生物活性，对有益阴道微生物群的破坏最小。天然产物 考虑与致病菌和有益菌以及化合物的相互作用的研究 负责，处于解决药用植物对女性健康功效的最前沿。我假设 traditional preparations of the identified species will vary in inhibition of the growth of pathogenic (Gardnerella 由于特殊代谢物的差异，阴道细菌）和有益（乳酸菌）阴道细菌。 此外，我假设不同国家之间在植物和女性健康实践方面会有相似之处。 由于加勒比地区有共同的植物群，海地和纽约的其他加勒比社区。所选植物 将分析这些物种对致病细菌、阴道加德纳菌以及 阴道中发现的重要有益乳酸菌。还将比较植物提取物以确定 使用 LC-MS 分析不同组织制剂中生物活性植物化学成分的变化 qToF 和随后的多元统计分析。与海地社区进行的民族植物学调查将 用于进一步确定加勒比妇女用于治疗妇科疾病的其他植物物种 用于继续实验室研究。这些数据将能够与其他加勒比地区进行跨文化比较 纽约市社区，还将评估 COVID-19 大流行对纽约市海地妇女的影响 他们使用药用植物来促进女性健康。来自阴道的植物的抗菌天然产物 完整的微生物群落可以为妇科疾病的持续治疗提供低成本且文化上合适的解决方案 状况。