Molecular Interactions and Imaging Core

分子相互作用和成像核心

• 批准号: 10647704
• 负责人: HENRY N HIGGS
• 金额: $ 44.63万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10647704
• 关键词: Actins; Adopted; Area; Award; Awareness; Binding; Biochemical; Biological Assay; Biology; Calorimetry; Cells; Centers of Research Excellence; Collaborations; Collection; Communities; Consultations; Core Facility; Cryoelectron Microscopy; DNA Repair; Data; Data Analyses; Development; Drosophila genus; Electron Microscopy; Electron Microscopy Facility; Embryo; Endocytosis; Ensure; Environment; Equipment; Experimental Designs; Extramural Activities; Faculty; Fluorescence Microscopy; Funding; Goals; Grant; Histone H3; Human; Image; Image Analysis; Imaging technology; In Situ; In Vitro; Individual; Influenza Hemagglutinin; Infrastructure; Institution; Laboratories; Lead; Location; Mammalian Cell; Mass Spectrum Analysis; Measures; Melanoma Cell; Methods; Microscopy; Mission; Modeling; Molecular; Molecular Structure; Monitor; Monoclonal Antibodies; Norris Cotton Cancer Center; Nuclear; Organelles; Participant; Phase; Pilot Projects; Play; Polymers; Population; Positioning Attribute; Process; Production; Program Reviews; Protein Dynamics; Protein Isoforms; Proteins; Proteomics; Recovery; Research; Research Infrastructure; Research Personnel; Research Project Grants; Resolution; Resources; Role; Scientist; Services; Specialist; Spectrum Analysis; Strategic Planning; Structure; Surface Plasmon Resonance; Surveys; T-Lymphocyte; Talents; Techniques; Technology; Time; Titrations; Training; Validation; Virus; Visualization; Water; Work; Workload; biophysical properties; biophysical techniques; cell motility; cellular imaging; cost; cost effective; design; experimental study; flexibility; fluorescence microscope; imaging study; improved; instrument; instrumentation; interest; intravital imaging; light microscopy; member; method development; molecular imaging; operation; polymerization; pressure; protein purification; recruit; success; superresolution microscopy; tool; tumor

The Molecular Interactions and Imaging Core (MIIC) increases the rigor and sophistication of bioMT research projects by facilitating the use of molecular binding assays and imaging technologies. One goal of the MIIC is to eliminate barriers to these technologies and provide advice in the optimal collection and analysis of the data. In phase I of this grant, MIIC has centralized oversight of existing resources and participated in the acquisition of new equipment, both within the bioMT facility and in conjunction with two existing microscopy cores at Dartmouth. We have hired staff to provide training, guidance, and experimental support in the characterization of molecular interactions. We have also hired two imaging navigators to help investigators with experimental design, image acquisition, and image analysis. These activities significantly enhanced the ability of our phase I research project leaders (RPLs) and all bioMT investigators to conduct quantitative binding and imaging studies at Dartmouth, and thus contributed to their extramural and academic success. Our plans for the next funding period have been guided by an inclusive and intensive program review process. In phase II, MIIC has four specific aims. Specific Aim 1 is to provide customized support to help the four RPLs attain their research goals and achieve independence. As active participants in the development of the four research projects, the director and research navigators engaged with the RPLs to comprehensively assess the methods required by each. From this survey we identified key technologies to quantify binding interactions, to visualize molecular locations and dynamics of proteins and organelles in cells, and to assess distribution of cells within tumors in situ. Specific Aim 2 is to expand our services to three key areas: mass spectrometry/proteomics, higher resolution cellular imaging by super-resolution microscopy and electron microscopy, and cryo-electron microscopy analysis of macromolecular structure. In all three areas, MIIC will continue the cost-effective practice of leveraging existing equipment at Dartmouth and elsewhere to accomplish these goals. Specific Aim 3 is to extend support for image analysis, which is an increasingly pressing need for many investigators. Specific Aim 4 prepares for sustainability of the Core in phase 3. The MIIC works closely with the Molecular Tools Core, which is housed in the same, newly renovated bioMT Core Facilities Suite. In addition, the MIIC has established strong working relationships with existing cores at Dartmouth (including several supported by other COBRE awards) as well as at other IDeA partner institutions. Within all four aims, the MIIC has been designed to adapt flexibly to technological needs that emerge as bioMT investigators make scientific progress. The MIIC will also share best practices and ensure up-to-date training for all bioMT investigators interested in monitoring, and in most cases quantifying, binding interactions of their biomolecules, whether as purified components or within cells. It will thus directly support all four bioMT Research Projects as well as affiliated bioMT faculty, and will enhance the research infrastructure available at Dartmouth and among our regional IDeA partners.

分子相互作用和成像核心（MIIC）增加了bioMT研究的严谨性和复杂性 通过促进分子结合分析和成像技术的使用，促进项目的发展。MIIC的一个目标是 消除这些技术的障碍，并就数据的最佳收集和分析提供咨询意见。 在该赠款的第一阶段，MIIC集中监督现有资源，并参与了收购 新设备，无论是在bioMT设施内，并结合两个现有的显微镜核心， 达特茅斯。我们已经聘请了工作人员提供培训，指导和实验支持的表征 分子间相互作用。我们还聘请了两名成像导航员来帮助调查人员进行实验性的 设计、图像采集和图像分析。这些活动大大提高了我们第一阶段的能力， 研究项目负责人（RPL）和所有bioMT研究者进行定量结合和成像研究 在达特茅斯，从而有助于他们的校外和学术上的成功。我们下一次融资的计划 在此期间，以包容性和密集的方案审查程序为指导。在第二阶段，MIIC有四个 具体目标。具体目标1是提供个性化的支持，以帮助四个RPL实现其研究目标 实现独立。作为四个研究项目发展的积极参与者， 以及研究导航员与可重复使用运载火箭合作，全面评估每种运载火箭所需的方法。 从这项调查中，我们确定了量化结合相互作用的关键技术， 以及细胞中蛋白质和细胞器的动力学，并原位评估肿瘤内细胞的分布。具体 目标2是将我们的服务扩展到三个关键领域：质谱/蛋白质组学，更高分辨率的细胞 超分辨率显微镜和电子显微镜成像，以及冷冻电子显微镜分析， 大分子结构在所有这三个领域，MIIC将继续采用具有成本效益的做法， 设备在达特茅斯和其他地方来实现这些目标。具体目标3是扩展对图像的支持 分析，这是许多调查人员越来越迫切的需要。具体目标4为可持续性做准备 第三阶段的核心MIIC与分子工具核心密切合作，分子工具核心位于同一个， 新装修的bioMT核心设施套房。此外，MIIC还建立了牢固的工作关系， 与达特茅斯（包括其他COBRE奖项支持的几个）以及其他IDEA的现有核心一起 伙伴机构。在所有四个目标中，MIIC被设计为灵活地适应技术需求 随着bioMT研究人员的科学进步而出现。MIIC还将分享最佳实践，并确保 为所有对监测感兴趣的bioMT研究人员提供最新培训，在大多数情况下， 它们的生物分子的相互作用，无论是作为纯化的组分还是在细胞内。它将直接支持所有 四个bioMT研究项目以及附属的bioMT教师，并将加强研究基础设施 可在达特茅斯和我们的区域IDEA合作伙伴中获得。