Cardiovascular regeneration and pioneer factors

心血管再生和先锋因素

基本信息

  • 批准号:
    10649338
  • 负责人:
  • 金额:
    $ 69.74万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2023
  • 资助国家:
    美国
  • 起止时间:
    2023-05-20 至 2027-04-30
  • 项目状态:
    未结题

项目摘要

Cardiovascular diseases are both common and deadly. For example, peripheral artery disease affects more than 10M Americans resulting in more than 150,000 limb amputations each year in the U.S. In addition, more than 300,000 patients have coronary artery bypass grafting (surgical revascularization). Current medical therapies for vascular disease include limb amputation and vascular bypass grafting--these therapeutic interventions have significant limitations. These diseases are chronic, debilitating, lethal and they warrant new and novel therapies. Previous studies have demonstrated the essential role for pioneer factors that modulate chromatin accessibility and thereby impact the binding of early transcriptional regulators for lineage specification. We have recently demonstrated that ETV2 is an essential pioneer factor for endothelial, vascular and blood lineages. We have used global and conditional gene disruption strategies, fate-mapping, gene editing, single cell RNA-seq, ATAC-seq and ChIP-seq assays to provide supportive data for this application. In addition, we defined an important ETV2-miR130a-PDGFRa cascade that governs endothelial development. Furthermore, our recent publications and our preliminary data support the overall hypothesis that ETV2 is a pioneer factor that regulates the specification of the endothelial lineage. In these proposed studies, we will use a number of unique genetic models that we have engineered and we take an innovative strategy to define the mechanisms whereby ETV2 functions as a pioneer factor to regulate cardiovascular regeneration. To examine our hypotheses, we will address the following specific aims: Specific Aim #1: Specific Aim #1: To further define the mechanisms whereby ETV2 functions as a pioneer factor during embryogenesis and reprogramming to the endothelial lineage; Specific Aim #2: To define the role of chromatin modifying factors and ETV2 during embryogenesis and reprogramming to the endothelial lineage and Specific Aim #3: To examine the factors that promote ETV2 mediated reprogramming of the endothelial lineage in vitro and in vivo. These aims will utilize our recently engineered genetic mouse models, ATAC-seq, MNase-seq, ChIP-seq, inducible mouse model, cardiac injury model in the adult mouse, novel and bioinformatics algorithms to comprehensively define the mechanisms whereby ETV2 functions as a pioneer factor and will serve as prelude for therapeutic initiatives to engineer and promote regeneration of the cardiovascular lineages. Given the tremendous morbidity and mortality of cardiovascular disease in our society, the potential impact of this proposal is significant.
心血管疾病既常见又致命。例如,外周动脉疾病影响更大

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
SeATAC: a tool for exploring the chromatin landscape and the role of pioneer factors.
  • DOI:
    10.1186/s13059-023-02954-5
  • 发表时间:
    2023-05-22
  • 期刊:
  • 影响因子:
    12.3
  • 作者:
    Gong, Wuming;Dsouza, Nikita;Garry, Daniel J.
  • 通讯作者:
    Garry, Daniel J.
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Daniel J. Garry其他文献

Porcine myogenesis in cloned wildtype and MYF5/MYOD/MYF6-null porcine embryo
克隆野生型和 MYF5/MYOD/MYF6 基因敲除猪胚胎中的猪肌发生
  • DOI:
    10.1038/s42003-025-07648-1
  • 发表时间:
    2025-02-11
  • 期刊:
  • 影响因子:
    5.100
  • 作者:
    Yong-Ho Choe;Satyabrata Das;Xiao Ma;Hyeonjeong Lee;Jacob R. Sorensen;Daniel B. Hoffman;Chan-Hee Jo;Casey P. Johnson;Nicolette Cassel;Daniel J. Garry;Sarah M. Greising;Mary G. Garry
  • 通讯作者:
    Mary G. Garry
Promoting cardiomyocyte proliferation for myocardial regeneration in large mammals
促进大型哺乳动物心肌细胞增殖以实现心肌再生
  • DOI:
    10.1016/j.yjmcc.2024.01.005
  • 发表时间:
    2024-03-01
  • 期刊:
  • 影响因子:
    4.700
  • 作者:
    Thanh Nguyen;Manuel Rosa-Garrido;Hesham Sadek;Daniel J. Garry;Jianyi (Jay) Zhang
  • 通讯作者:
    Jianyi (Jay) Zhang
Benchmarked approaches for cell lineage reconstructions of in vitro dividing cells and in 1 silico models of Caenorhabditis elegans and Mus musculus developmental trees.
体外分裂细胞和秀丽隐杆线虫和小家鼠发育树的 1 计算机模型中细胞谱系重建的基准方法。
  • DOI:
  • 发表时间:
    2021
  • 期刊:
  • 影响因子:
    0
  • 作者:
    W. Gong;Alejandro A. Granados;Jingyuan Hu;Matthew G. Jones;Ofir Raz;Irepan Salvador;Hanrui Zhang;Ke;Il;R. Retkute;Alidivinas Prusokas;Augustinas Prusokas;Alex Khodaverdian;Richard Zhang;Suhas S. P. Rao;Robert Wang;P. Rennert;V. Saipradeep;N. Sivadasan;Aditya Rao;Thomas Joseph;Rajgopal Srinivasan;Jiajie Peng;Lu Han;Xuequn Shang;Daniel J. Garry;Thomas Yu;Verena Chung;M. Mason;Zhandong Liu;Y. Guan;N. Yosef;J. Shendure;M. Telford;E. Shapiro;M. Elowitz;P. Meyer
  • 通讯作者:
    P. Meyer
The Lillehei Heart Institute: Building on the Shoulders of Giants
Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation”
  • DOI:
    10.1186/s13059-021-02320-3
  • 发表时间:
    2021-04-07
  • 期刊:
  • 影响因子:
    9.400
  • 作者:
    Channabasavaiah B. Gurumurthy;Aidan R. O’Brien;Rolen M. Quadros;John Adams;Pilar Alcaide;Shinya Ayabe;Johnathan Ballard;Surinder K. Batra;Marie-Claude Beauchamp;Kathleen A. Becker;Guillaume Bernas;David Brough;Francisco Carrillo-Salinas;Wesley Chan;Hanying Chen;Ruby Dawson;Victoria DeMambro;Jinke D’Hont;Katharine Dibb;James D. Eudy;Lin Gan;Jing Gao;Amy Gonzales;Anyonya Guntur;Huiping Guo;Donald W. Harms;Anne Harrington;Kathryn E. Hentges;Neil Humphreys;Shiho Imai;Hideshi Ishii;Mizuho Iwama;Eric Jonasch;Michelle Karolak;Bernard Keavney;Nay-Chi Khin;Masamitsu Konno;Yuko Kotani;Yayoi Kunihiro;Imayavaramban Lakshmanan;Catherine Larochelle;Catherine B. Lawrence;Lin Li;Volkhard Lindner;Xian-De Liu;Gloria Lopez-Castejon;Andrew Loudon;Jenna Lowe;Loydie Jerome-Majeweska;Taiji Matsusaka;Hiromi Miura;Yoshiki Miyasaka;Benjamin Morpurgo;Katherine Motyl;Yo-ichi Nabeshima;Koji Nakade;Toshiaki Nakashiba;Kenichi Nakashima;Yuichi Obata;Sanae Ogiwara;Mariette Ouellet;Leif Oxburgh;Sandra Piltz;Ilka Pinz;Moorthy P. Ponnusamy;David Ray;Ronald J. Redder;Clifford J. Rosen;Nikki Ross;Mark T. Ruhe;Larisa Ryzhova;Ane M. Salvador;Sabrina Shameen Alam;Radislav Sedlacek;Karan Sharma;Chad Smith;Katrien Staes;Lora Starrs;Fumihiro Sugiyama;Satoru Takahashi;Tomohiro Tanaka;Andrew Trafford;Yoshihiro Uno;Leen Vanhoutte;Frederique Vanrockeghem;Brandon J. Willis;Christian S. Wright;Yuko Yamauchi;Xin Yi;Kazuto Yoshimi;Xuesong Zhang;Yu Zhang;Masato Ohtsuka;Satyabrata Das;Daniel J. Garry;Tino Hochepied;Paul Thomas;Jan Parker-Thornburg;Antony D. Adamson;Atsushi Yoshiki;Jean-Francois Schmouth;Andrei Golovko;William R. Thompson;K. C. Kent Lloyd;Joshua A. Wood;Mitra Cowan;Tomoji Mashimo;Seiya Mizuno;Hao Zhu;Petr Kasparek;Lucy Liaw;Joseph M. Miano;Gaetan Burgio
  • 通讯作者:
    Gaetan Burgio

Daniel J. Garry的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Daniel J. Garry', 18)}}的其他基金

Project 2 - Shh and Etv2 Signaling Pathways and Cardiovascular Repair in Mouse and Pig
项目 2 - Shh 和 Etv2 信号通路以及小鼠和猪的心血管修复
  • 批准号:
    10493839
  • 财政年份:
    2022
  • 资助金额:
    $ 69.74万
  • 项目类别:
Project 2 - Shh and Etv2 Signaling Pathways and Cardiovascular Repair in Mouse and Pig
项目 2 - Shh 和 Etv2 信号通路以及小鼠和猪的心血管修复
  • 批准号:
    10677734
  • 财政年份:
    2022
  • 资助金额:
    $ 69.74万
  • 项目类别:
Bioengineering Strategies for Cardiovascular Disease
心血管疾病的生物工程策略
  • 批准号:
    10227924
  • 财政年份:
    2019
  • 资助金额:
    $ 69.74万
  • 项目类别:
Bioengineering Strategies for Cardiovascular Disease
心血管疾病的生物工程策略
  • 批准号:
    10468711
  • 财政年份:
    2019
  • 资助金额:
    $ 69.74万
  • 项目类别:
Regulatory Mechanisms of Endothelial Development and Regeneration
内皮发育和再生的调节机制
  • 批准号:
    9002076
  • 财政年份:
    2014
  • 资助金额:
    $ 69.74万
  • 项目类别:
Regulatory Mechanisms of Endothelial Development and Regeneration
内皮发育和再生的调节机制
  • 批准号:
    8668377
  • 财政年份:
    2014
  • 资助金额:
    $ 69.74万
  • 项目类别:
Regulatory Mechanisms of Endothelial Development and Regeneration
内皮发育和再生的调节机制
  • 批准号:
    8827844
  • 财政年份:
    2014
  • 资助金额:
    $ 69.74万
  • 项目类别:
Midwestern Progenitor Cell Consortium
中西部祖细胞联盟
  • 批准号:
    8663942
  • 财政年份:
    2009
  • 资助金额:
    $ 69.74万
  • 项目类别:
Midwestern Progenitor Cell Consortium
中西部祖细胞联盟
  • 批准号:
    7833748
  • 财政年份:
    2009
  • 资助金额:
    $ 69.74万
  • 项目类别:
Midwestern Progenitor Cell Consortium
中西部祖细胞联盟
  • 批准号:
    8494683
  • 财政年份:
    2009
  • 资助金额:
    $ 69.74万
  • 项目类别:

相似海外基金

Co-designing a lifestyle, stop-vaping intervention for ex-smoking, adult vapers (CLOVER study)
为戒烟的成年电子烟使用者共同设计生活方式、戒烟干预措施(CLOVER 研究)
  • 批准号:
    MR/Z503605/1
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Research Grant
Early Life Antecedents Predicting Adult Daily Affective Reactivity to Stress
早期生活经历预测成人对压力的日常情感反应
  • 批准号:
    2336167
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Standard Grant
RAPID: Affective Mechanisms of Adjustment in Diverse Emerging Adult Student Communities Before, During, and Beyond the COVID-19 Pandemic
RAPID:COVID-19 大流行之前、期间和之后不同新兴成人学生社区的情感调整机制
  • 批准号:
    2402691
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Standard Grant
Elucidation of Adult Newt Cells Regulating the ZRS enhancer during Limb Regeneration
阐明成体蝾螈细胞在肢体再生过程中调节 ZRS 增强子
  • 批准号:
    24K12150
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Migrant Youth and the Sociolegal Construction of Child and Adult Categories
流动青年与儿童和成人类别的社会法律建构
  • 批准号:
    2341428
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Standard Grant
Understanding how platelets mediate new neuron formation in the adult brain
了解血小板如何介导成人大脑中新神经元的形成
  • 批准号:
    DE240100561
  • 财政年份:
    2024
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Discovery Early Career Researcher Award
Laboratory testing and development of a new adult ankle splint
新型成人踝关节夹板的实验室测试和开发
  • 批准号:
    10065645
  • 财政年份:
    2023
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Collaborative R&D
Usefulness of a question prompt sheet for onco-fertility in adolescent and young adult patients under 25 years old.
问题提示表对于 25 岁以下青少年和年轻成年患者的肿瘤生育力的有用性。
  • 批准号:
    23K09542
  • 财政年份:
    2023
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification of new specific molecules associated with right ventricular dysfunction in adult patients with congenital heart disease
鉴定与成年先天性心脏病患者右心室功能障碍相关的新特异性分子
  • 批准号:
    23K07552
  • 财政年份:
    2023
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Issue identifications and model developments in transitional care for patients with adult congenital heart disease.
成人先天性心脏病患者过渡护理的问题识别和模型开发。
  • 批准号:
    23K07559
  • 财政年份:
    2023
  • 资助金额:
    $ 69.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了