Targeting Polo-Like Kinase 1 in Prostate Cancer to Enhance Therapeutic Efficacy

标题：靶向前列腺癌中的Polo样激酶1以提高治疗效果 摘要 前列腺癌（PCa）是美国男性癌症死亡的第二大常见原因。过去 几十年来，雄激素剥夺治疗一直是患有 晚期PCa;然而，大多数患者发展为去势抵抗的预后不良阶段， 前列腺癌（CRPC），最终导致死亡。目前CRPC的治疗药物 患者的生存期仅延长约2.5 - 5个月。因此，新的和有效的治疗策略， CRPC患者急需治疗。这项研究的长期目标是确定药物信号 为CRPC患者提供更有效的治疗选择。目标是确定 Plk 1/p62/Nrf 2信号通路在CRPC中的作用，并探索可能的治疗策略， 有利于CRPC患者。核心假设是Nrf 2是由非经典机制激活的 涉及CRPC中Plk 1引起的p62磷酸化。我们的假设将通过追求三个具体目标来检验： (1)以确定Plk 1作为细胞对氧化应激反应的调节剂的新作用。(2)检查的作用 Plk 1/p62/Nrf 2轴在小鼠肿瘤生长中的作用。以及（3）Plk 1/p62/Nrf 2信号通路的抑制是否 CRPC通路增强CRPC治疗的功效。预期的结果将揭示一种新的分子机制 揭示Plk 1在前列腺癌进展中的作用，为Plk 1抑制剂的应用开辟新的途径 作为一种治疗选择。该团队在小鼠模型中的Plk 1，癌细胞生物学中的Plk 1，PCa 病理学，GU肿瘤学和生物统计学，将能够及时完成拟议的研究。

Title: Targeting Polo-like kinase 1 in prostate cancer to enhance therapeutic efficacy Abstract Prostate cancer (PCa) is the second most common cause of cancer death in men in the US. Over the past several decades, androgen deprivation therapy has been the primary therapeutic option for patients with advanced PCa; however, the majority of patients developed into a poor prognosis stage of castration-resistant prostate cancer (CRPC), which eventually led to mortality. And the current therapeutic agents for CRPC patients only extend survival by around 2.5 - 5 months. Therefore, novel and effective therapeutic strategies for CRPC patients are urgently needed. The long-term goals of this study are to identify druggable signaling pathways that offer more effective treatment options for patients with CRPC. The objective is to identify the role of Plk1/p62/Nrf2 signaling pathway in CRPC, and to exploit the potentially therapeutic strategies that could be benefit the CRPC patients. The central hypothesis is that Nrf2 is activated by a noncanonical mechanism involving p62 phosphorylation by Plk1 in CRPC. Our hypothesis will be tested by pursuing Three Specific Aims: (1) to determine the novel role of Plk1 as regulator of cellular response to oxidative stress. (2) examine the role of Plk1/p62/Nrf2 axis in tumor growth in mouse models. And (3) whether inhibition of Plk1/p62/Nrf2 signaling pathway enhance the efficacy of CRPC therapy. The expected results will reveal a novel molecular mechanism to show how Plk1 contributes to PCa progress, and will open up new avenues for application of Plk1 inhibitors as a therapeutic option. The team, with expertise in Plk1 in mouse models, Plk1 in cancer cell biology, PCa pathology, GU oncology and biostatistics, will be able to finish the proposed research in a timely manner.