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A novel Lyme disease vaccine

一种新型莱姆病疫苗

基本信息

批准号：
10515700
负责人：
Joseph Edgar Craft
金额：
$ 64.81万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-07 至 2027-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10515700
关键词：
Animal Model Animals Antibody Response Antigens Black-legged Tick Borrelia burgdorferi CD4 Positive T Lymphocytes Cavia Complement Data Dermal Development Exposure to Fertility Goals Immune response Immunization Immunize Immunologics Infection Infection prevention Integration Host Factors Kinetics Knowledge Lead Lyme Disease Lyme Disease Vaccines Lymphocyte Activation Messenger RNA Modeling Molting Names Oryctolagus cuniculus Pathway interactions Play Prevention Process Proteins Publishing RNA Interference RNA vaccination Reagent Redness Resistance Role Saliva Salivary Proteins Skin Systemic infection Tick-Borne Infections Ticks Vaccination experimental study i(19)in vivo lipid nanoparticle lymphocyte proliferation nanoparticle novel novel vaccines pathogen prevent response saliva mediated tick bite tick saliva tick transmission transcriptome transcriptomics transmission process vaccination strategy

项目摘要

PROJECT SUMMARY A novel vaccine for Lyme disease Acquired resistance to Ixodes scapularis, commonly known as “tick resistance”, can prevent transmission of Borrelia burgdorferi, the Lyme disease agent. We have shown that tick resistance can be generated in guinea pigs (1) repeatedly exposed to I. scapularis, (2) immunized with I. scapularis saliva, or (3) immunized with a lipid nanoparticle containing mRNAs encoding 19 saliva proteins (19ISP). In addition, salivary protein 14 (Salp14), a component of 19ISP, elicits partial tick resistance. The goal of this proposal is to identify the specific proteins in I. scapularis saliva that elicit tick resistance and demonstrate that vaccination with these proteins can prevent B. burgdorferi infection. We will delineate the importance of Salp14 and additional saliva proteins in tick resistance, determine the host responses that are associated with tick resistance, and examine whether vaccination with saliva proteins or mRNAs encoding each saliva protein can prevent B. burgdorferi infection. These studies will lead to new knowledge to aid in the development of a novel vaccine which may prevent Lyme disease and, possibly, other tick-borne infections.

项目总结一种新的莱姆病疫苗对肩部硬蜱的获得性抵抗力，俗称“扁虱抵抗力”，可以预防莱姆病病原体伯氏疏螺旋体的传播。我们有结果表明，在反复暴露于I的豚鼠(1)中，可以产生蜱抗性。肩部，(2)用我肩部唾液免疫，或(3)用脂类免疫含有编码19种唾液蛋白的mRNAs的纳米颗粒(19isp)。此外, 唾液蛋白14(Salp14)是19isp的一种成分，可引起部分硬蜱抗药性。这项提议的目标是鉴定肩胛肌中的特定蛋白质。唾液会引起壁虱的抵抗力，并证明用这些疫苗接种蛋白质可以预防伯氏杆菌感染。我们将描述以下内容的重要性 Salp14和额外的唾液蛋白决定了寄主的反应并检查是否用唾液接种疫苗编码每种唾液蛋白的蛋白质或mRNAs可以预防伯氏杆菌感染。这些研究将带来新的知识，以帮助开发新的疫苗这可能会预防莱姆病，可能还会预防其他由壁虱传播的感染。