Computational tools for uniform processing and integration of human reference atlas data [2 of 5]

用于统一处理和集成人类参考图集数据的计算工具 [2 of 5]

• 批准号: 10534347
• 负责人: Benedict Paten
• 金额: $ 109.21万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10534347
• 关键词: Atlases; Data; Human; Text; computerized tools

The HuBMAP Computational Tools Component is responsible for development of standardized processing pipeline for all HuBMAP data, which is the foundation that enables joint storage, indexing, display, querying and mapping of HuBMAP datasets. A key innovative aspect that we have championed and, which as far as we know is unique for large consortia with several groups, platforms and data modalities is the fact that all HuBMAP pipelines we implemented provide uniform processing of all data from the same modality. Specifically, for each of these modalities we use the same computational algorithm for initial and downstream processing. For example, all scRNA-Seq datasets, regardless of which of the 7 platforms they were generated by, are processed by our Salmon quantification pipeline. This makes integration and comparison much easier across all HuBMAP data. In addition, all downstream analysis including dimensionality reduction, clustering, differential expression etc. are also performed using the same methods for all platforms making it easy to organize the display of the data on the portal and to index the data for quantitative queries. Similar pipelines have been developed for other modalities. In the production phase of the HuBMAP Consortium, we will extend these computational pipelines to support additional data types from new tissue mapping centers and other data providers. We will additionally extend our focus beyond single-cell image segmentation to support the mapping efforts of the Human Reference Atlas (HRA) collaboration, producing hierarchical links from cells to functional tissue units to higher-level anatomical structures.

HuBMAP计算工具组件负责标准化开发