Neotropical Primate Reagent Resource

总结 非人灵长类动物（NHP）在历史上一直是疾病的重要动物模型， 无法在啮齿动物中进行评估的新疗法和疫苗。新热带灵长类动物用于生物医学 在美国的研究频率低于旧世界灵长类动物，但对这些动物的兴趣越来越大， 由于某些临床疾病的密切重演和简单的饲养考虑，研究 包括绒猴和松鼠猴在内的大部分NIH资助的研究都是以新热带为特征的， 灵长类动物在几个领域，包括神经退行性疾病，传染病和衰老。 在这个资源申请中，我们建议开发一些绒猴和松鼠猴抗体， 解决项目目标和可用试剂之间的差距。物种匹配的抗体可以消耗 靶向淋巴细胞亚群，使研究人员能够探测特定免疫群体对 发展、保护和恢复。区分抗体类别和亚类的诊断抗体， 细胞表面生物标志物和可溶性介质将减少由不良试剂可及性所施加的限制。到 衍生这些试剂，松鼠猴和绒猴免疫球蛋白和Fcg受体库将被 详尽的定义和特征。 这里描述的抗体资源可以通过以下方式提高新热带灵长类动物转化研究的标准： 为研究人员提供了有效的工具，以评估感染或炎症模型中的免疫反应， 疾病

Summary Nonhuman primates (NHPs) have historically served as crucial animal models for diseases, and for testing of new therapies and vaccines that cannot be evaluated in rodents. Neotropical primates are used in biomedical research less frequently in the U.S. than Old World primates, but there is growing interest in these animals as models due to the close recapitulation of certain clinical diseases and simpler husbandry considerations. Studies involving marmosets and squirrel monkeys comprise the bulk of NIH-funded research featuring neotropical primates in several areas, including neurodegenerative disorders, infectious diseases, and aging. In this resource application, we propose to develop a number of marmoset and squirrel monkey antibodies to address the gap between project goals and available reagents. Species-matched antibodies can deplete targeted lymphocyte subsets and allow investigators to probe the impact of specific immune populations on progression, protection, and recovery. Diagnostic antibodies that distinguish antibody classes and subclasses, cell surface biomarkers, and soluble mediators will reduce limitations imposed by poor reagent accessibility. To derive these reagents, the squirrel monkey and marmoset immunoglobulin and Fcg receptor repertoire will be exhaustively defined and characterized. The antibody resources described here can raise the bar on neotropical primate translational research by providing investigators validated tools to evaluate the immune response in models of infectious or inflammatory disease.