Neotropical Primate Reagent Resource

摘要 非人灵长类动物(NHP)历史上一直是疾病的重要动物模型，并用于测试 无法在啮齿动物身上进行评估的新疗法和疫苗。新热带灵长类动物被用于生物医学 在美国，研究的频率低于东半球的灵长类动物，但人们对这些动物的兴趣越来越大，因为 由于对某些临床疾病的严密总结和更简单的饲养考虑，这些模型。研究 涉及绒猴和松鼠猴的研究构成了美国国立卫生研究院资助的以新热带为特征的研究的大部分 灵长类动物在几个领域，包括神经退行性疾病，传染病，和衰老。 在这一资源申请中，我们建议开发一些猴和绒猴的抗体来 解决项目目标和可用试剂之间的差距。物种匹配的抗体可以耗尽 靶向淋巴细胞亚群，并允许研究人员探索特定免疫群体对 进展、保护和恢复。区分抗体类别和亚类的诊断抗体， 细胞表面生物标记物和可溶性介体将减少试剂可及性差带来的限制。至 派生出这些试剂后，松鼠、猴和绒猴的免疫球蛋白和FCG受体将被 详尽地定义和描述的。 这里描述的抗体资源可以提高新热带灵长类翻译研究的门槛 为研究人员提供有效的工具来评估感染或炎症模型中的免疫反应 疾病。

Summary Nonhuman primates (NHPs) have historically served as crucial animal models for diseases, and for testing of new therapies and vaccines that cannot be evaluated in rodents. Neotropical primates are used in biomedical research less frequently in the U.S. than Old World primates, but there is growing interest in these animals as models due to the close recapitulation of certain clinical diseases and simpler husbandry considerations. Studies involving marmosets and squirrel monkeys comprise the bulk of NIH-funded research featuring neotropical primates in several areas, including neurodegenerative disorders, infectious diseases, and aging. In this resource application, we propose to develop a number of marmoset and squirrel monkey antibodies to address the gap between project goals and available reagents. Species-matched antibodies can deplete targeted lymphocyte subsets and allow investigators to probe the impact of specific immune populations on progression, protection, and recovery. Diagnostic antibodies that distinguish antibody classes and subclasses, cell surface biomarkers, and soluble mediators will reduce limitations imposed by poor reagent accessibility. To derive these reagents, the squirrel monkey and marmoset immunoglobulin and Fcg receptor repertoire will be exhaustively defined and characterized. The antibody resources described here can raise the bar on neotropical primate translational research by providing investigators validated tools to evaluate the immune response in models of infectious or inflammatory disease.