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Neotropical Primate Reagent Resource

新热带灵长类试剂资源

基本信息

批准号：
10662259
负责人：
Kathleen Engelman
金额：
$ 56.53万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10662259
关键词：
Address Affinity African Aging Animal Disease Models Animal Model Animals Antibodies Antibody Response Area Asian Basic Science Biological Biological Markers Biomedical Research CD8B1 gene Callithrix Callithrix jacchus jacchus Cell surface Cells Clinical Communicable Diseases Development Disease Disease model Engineering Funding Generations Genetic Polymorphism Goals IgG Receptors Immune Immune Targeting Immune response Immune system Immunoglobulin A Immunoglobulin G Immunoglobulin M Immunoglobulins Immunologics Inflammatory Lymphocyte Depletion Lymphocyte Subset MS4A1 gene Mediator Metabolic Diseases Modeling Modification Monoclonal Antibodies Neurodegenerative Disorders Population Primates Reagent Recovery Research Research Contracts Research Personnel Resources Rodent Saimiri Surface Antigens Testing Time Transgenic Animals Translational Research United States National Institutes of Health antibody diagnostic cross reactivity drug development in vivo infectious disease model interest nonhuman primate novel therapeutics novel vaccines receptor tool

项目摘要

Summary Nonhuman primates (NHPs) have historically served as crucial animal models for diseases, and for testing of new therapies and vaccines that cannot be evaluated in rodents. Neotropical primates are used in biomedical research less frequently in the U.S. than Old World primates, but there is growing interest in these animals as models due to the close recapitulation of certain clinical diseases and simpler husbandry considerations. Studies involving marmosets and squirrel monkeys comprise the bulk of NIH-funded research featuring neotropical primates in several areas, including neurodegenerative disorders, infectious diseases, and aging. In this resource application, we propose to develop a number of marmoset and squirrel monkey antibodies to address the gap between project goals and available reagents. Species-matched antibodies can deplete targeted lymphocyte subsets and allow investigators to probe the impact of specific immune populations on progression, protection, and recovery. Diagnostic antibodies that distinguish antibody classes and subclasses, cell surface biomarkers, and soluble mediators will reduce limitations imposed by poor reagent accessibility. To derive these reagents, the squirrel monkey and marmoset immunoglobulin and Fcg receptor repertoire will be exhaustively defined and characterized. The antibody resources described here can raise the bar on neotropical primate translational research by providing investigators validated tools to evaluate the immune response in models of infectious or inflammatory disease.

总结非人灵长类动物（NHP）在历史上一直是疾病的重要动物模型，无法在啮齿动物中进行评估的新疗法和疫苗。新热带灵长类用于生物医学在美国的研究频率低于旧世界灵长类动物，但对这些动物的兴趣越来越大，由于某些临床疾病的密切重演和简单的饲养考虑，研究包括绒猴和松鼠猴在内的大部分NIH资助的研究都是以新热带为特征的，灵长类动物在几个领域，包括神经退行性疾病，传染病和衰老。在这个资源申请中，我们建议开发一些绒猴和松鼠猴抗体，解决项目目标和可用试剂之间的差距。物种匹配的抗体可以消耗靶向淋巴细胞亚群，使研究人员能够探测特定免疫群体对发展、保护和恢复。区分抗体类别和亚类的诊断抗体，细胞表面生物标志物和可溶性介质将减少由不良试剂可及性所施加的限制。到衍生这些试剂，松鼠猴和绒猴免疫球蛋白和Fcg受体库将被详尽的定义和特征。这里描述的抗体资源可以通过以下方式提高新热带灵长类动物转化研究的标准：为研究人员提供了有效的工具，以评估感染或炎症模型中的免疫反应，疾病