权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Engineering Brain Cancer in a Dish: Hydrogel-based 3D in vitro Models for Pediatric Brain Tumor

在培养皿中改造脑癌：基于水凝胶的小儿脑肿瘤 3D 体外模型

基本信息

批准号：
10539308
负责人：
Sauradeep Sinha
金额：
$ 4.07万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-01 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10539308
关键词：
3-Dimensional Address Adherent Culture Adhesions Adhesives Adult Affect Animal Model Automobile Driving Biochemical Biomedical Engineering Biomimetics Brain Brain Neoplasms Brain Stem Cancer Biology Cancer Model Cell Communication Cells Cessation of life Child Childhood Childhood Brain Neoplasm Clinical Coculture Techniques Collaborations Complex Cues Development Diffuse intrinsic pontine glioma Discipline Disease Dose Engineering Environment Extracellular Matrix Faculty Future Glioblastoma Goals Histone Deacetylase Histone Deacetylation Hydrogels In Vitro Integrins Invaded Ligands Malignant Childhood Neoplasm Malignant neoplasm of brain Mentorship Modeling Molecular Mus Neoplasms Oncogenic Outcome Pathway interactions Pharmaceutical Preparations Phenotype Physicians Play Pontine structure Positioning Attribute Proliferating Proteins RNA Receptor Inhibition Reporting Resistance development Role Scientist Signal Transduction Site Solid Neoplasm Survival Rate Testing Therapeutic Time Training Treatment outcome Tumor Cell Invasion Work anticancer research cancer cell career development cell behavior cost disease phenotype drug resistance development effective therapy efficacy validation improved improved outcome in vitro Model in vivo inhibitor materials science mechanical signal migration mouse model neoplastic cell nerve stem cell neurosurgery novel novel therapeutic intervention novel therapeutics receptor response synergism therapeutic candidate therapeutic target three-dimensional modeling tumor growth tumor microenvironment

项目摘要

Diffuse intrinsic pontine gliomas (DIPG) are a highly aggressive pediatric brain tumor of the ventral pons (brain stem), with a five-year survival rate of less than 1% and a median survival of only 9 months [1,2]. While significant improvement in survival has been achieved in treating other forms of pediatric cancer, survival rate for DIPG has not changed in over three decades [1]. While the brain tumor niche itself is a 3D, multi-factorial environment, previous attempts have relied on standard 2D monolayer culture or animal models to mimic the disease phenotype. However, increasing evidence has shown that cancer cell behavior in 2D differs substantially from the in vivo phenotype [3]; whereas animal models are costly, lengthy to produce, and often cumbersome for mechanistic studies. Furthermore, previous studies were done almost exclusively with adult brain tumor cells, whereas adult and pediatric brain tumors have been shown to demonstrate distinct phenotypes in their sites of origin, clinical presentations and molecular mechanisms [4]. Through working at the interface of bioengineering, materials science, cancer biology, neurosurgery, and animal models, the goals of this proposal are to develop hydrogels with optimized niche cues to support DIPG proliferation and invasion in 3D, and to harness such in vitro model for elucidating the role of integrin receptors and cell-cell interactions in driving DIPG progression. The efficacy of blocking specific integrin receptors for inhibiting DIPG progression will be further validated in vivo using our established mouse models. I hypothesize that blocking DIPG adhesion through specific integrin receptors would inhibit DIPG proliferation and invasion in 3D. Furthermore, there is a need to find and advance combinational therapeutic strategies since DIPG has been shown to ultimately develop resistance even to promising single targeting regimes like HDAC inhibition [7,8]. I hypothesize that blocking integrin receptor would synergize with HDAC inhibition to further improve treatment outcome of DIPG by disrupting two distinct oncogenic pathways. I further hypothesize that 3D co-culture of DIPG with neural progenitor cells (NPCs) in 3D would enhance DIPG invasion, a phenotype that mimics the in vivo response. To test these hypotheses, I propose to: (1) Develop 3D hydrogels with brain-mimicking stiffness and optimized adhesive ligands that support DIPG proliferation, invasion and drug responses in 3D; (2) Evaluate the effects of blocking specific integrin receptors required for DIPG adhesion in inhibiting DIPG invasion using our 3D hydrogels models, and validate the efficacy using a mouse DIPG model; and (3) Develop a 3D co-culture model to recapitulate NPC-induced DIPG invasion, and identify key signals impacted by NPC/DIPG interactions using RNA microarray. The outcomes of the proposed work would lead to the development of a bioengineered 3D in vitro model for DIPG with controlled cell-matrix and cell-cell interactions that mimics in vivo phenotype. Under the mentorship of a team of basic and physician scientists, with complimentary expertise, I will gain valuable interdisciplinary trainings and be uniquely positioned to carry out the proposed work.

弥漫性内在脑桥胶质瘤（DIPG）是一种高度侵袭性脑桥腹侧的儿童脑肿瘤