Ultra-low-temperature (6 K) static NMR-DNP for metalloproteins, proteins in cells, and materials
用于金属蛋白、细胞中蛋白质和材料的超低温 (6 K) 静态 NMR-DNP
基本信息
- 批准号:10546201
- 负责人:
- 金额:$ 29.96万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-03-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:Alzheimer&aposs DiseaseAmyloid FibrilsAntibioticsAreaAwarenessBee VenomsBindingBiologicalBiomedical ResearchBudgetsCellsCellular MembraneCountryDNA Repair GeneDataDevelopmentElectronsEquipmentExtremely High Frequency Radio WavesHealthHumanInflammasomeLaboratoriesLipidsMagicMagnetismMeasuresMembrane ProteinsMetalloproteinsMethodsMolecularMotionNisinNuclearNuclear Magnetic ResonanceOpticsPeptidesPharmacologic SubstancePhasePlayPriceProlineProteinsRelaxationReportingResearchResearch PersonnelResolutionRoleSamplingSignal TransductionSmall Business Innovation Research GrantSolidSourceStructureSystemTechniquesTemperatureamyloid structurecold temperaturecostdesignenhancing factorexperimental studyimprovedin silicoinstrumentationmacromoleculematerials sciencemicrowave electromagnetic radiationnovelpeptide structureprototyperestraintsimulationsolid statesolid state nuclear magnetic resonancestructural biologytoolwaveguide
项目摘要
Ultra-low-temperature (6 K) static NMR-DNP for metalloproteins, proteins in cells, and materials
Abstract
The critical importance of solid-state NMR (ssNMR) was recently demonstrated by, after nearly two decades
of intense efforts, yielding the first atomic-resolution structures of the A40 and A42 amyloid fibrils that play a
crucial role in Alzheimer’s Disease (AD). Challenges posed by the inherently low sensitivity of NMR can be
improved by reducing the sample and circuit temperature to below 100 K, or preferably below 35 K – also
known as Ultra Low Temperature (ULT). Combining ULT NMR with another emerging technique, denoted as
dynamic nuclear polarization (DNP), shows enormous promise for expanding the role of ssNMR by providing
significant S/N enhancements in many cases. However, despite the huge gain in S/N that is possible from
DNP, also performed at ~90 K, there are still only a handful of high-field MAS-DNP systems in the U.S. – pri-
marily because they are so expensive ($3-10M). Further, no commercially available instrumentation is availa-
ble for NMR experiments below 90 K, let alone ULT experiments combined with DNP. A huge step forward
would include the development of a high-mode THz cavity for more efficient microwave delivery to the sample,
thus enabling the use of a lower power and lower cost microwave source. An area of ssNMR that employs the
use of non-spinning (static) samples would benefit immensely from the availability of such a commercially built
ULT probe, with option for inclusion of DNP. Further, the static probe can serve as a prototype for a MAS ver-
sion of such a probe. The developments proposed under this project aim to reduce entry level cost into DNP
by more than an order of magnitude while reducing certain operational challenges.
超低温(6 K)静态NMR-DNP金属蛋白,细胞中的蛋白质和材料
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Francis DAVID Doty其他文献
Francis DAVID Doty的其他文献
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{{ truncateString('Francis DAVID Doty', 18)}}的其他基金
A Novel Waveguide to Enable MAS-DNP-NMR in Standard-bore High-field Magnets
一种新型波导,可在标准孔径高场磁体中实现 MAS-DNP-NMR
- 批准号:
10081009 - 财政年份:2020
- 资助金额:
$ 29.96万 - 项目类别:
A Novel Waveguide to Enable MAS-DNP-NMR in Standard-bore High-field Magnets
一种新型波导,可在标准孔径高场磁体中实现 MAS-DNP-NMR
- 批准号:
10602643 - 财政年份:2020
- 资助金额:
$ 29.96万 - 项目类别:
A Reliable Switched Angle Spinning (SAS) Probe with Gradients (PFG) for Proteins in Solid-State NMR
用于固态 NMR 中蛋白质的可靠的带梯度 (PFG) 的转角旋转 (SAS) 探针
- 批准号:
10456218 - 财政年份:2018
- 资助金额:
$ 29.96万 - 项目类别:
A Reliable Switched Angle Spinning (SAS) Probe with Gradients (PFG) for Proteins in Solid-State NMR
用于固态 NMR 中蛋白质的可靠的带梯度 (PFG) 的转角旋转 (SAS) 探针
- 批准号:
10667507 - 财政年份:2018
- 资助金额:
$ 29.96万 - 项目类别:
A Reliable Switched Angle Spinning (SAS) Probe with Gradients (PFG) for Proteins in Solid-State NMR
用于固态 NMR 中蛋白质的可靠的带梯度 (PFG) 的转角旋转 (SAS) 探针
- 批准号:
10325061 - 财政年份:2018
- 资助金额:
$ 29.96万 - 项目类别:
A Novel Millimeter-wave (mmw) DNP/EPR Front-end Compatible with Versatile High-field NMR Probes
与多功能高场 NMR 探头兼容的新型毫米波 (mmw) DNP/EPR 前端
- 批准号:
9343460 - 财政年份:2017
- 资助金额:
$ 29.96万 - 项目类别:
An H/F/X/Y Fast-MAS NMR Probe Particularly for Alzheimer's and Cancer Research
特别适用于阿尔茨海默病和癌症研究的 H/F/X/Y Fast-MAS NMR 探针
- 批准号:
9908407 - 财政年份:2016
- 资助金额:
$ 29.96万 - 项目类别:
A Quad-Fast-MAS probe for Dramatically Improved Biomolecular Structure Determinations
用于显着改进生物分子结构测定的 Quad-Fast-MAS 探针
- 批准号:
9045315 - 财政年份:2016
- 资助金额:
$ 29.96万 - 项目类别:
An H/F/X/Y Fast-MAS NMR Probe Particularly for Alzheimer's and Cancer Research
特别适用于阿尔茨海默病和癌症研究的 H/F/X/Y Fast-MAS NMR 探针
- 批准号:
10224643 - 财政年份:2016
- 资助金额:
$ 29.96万 - 项目类别:
An HXYZ-g HR-Fast-MAS probe for Dramatically Improved Biomolecular Structure Determinations
用于显着改进生物分子结构测定的 HXYZ-g HR-Fast-MAS 探针
- 批准号:
9988618 - 财政年份:2016
- 资助金额:
$ 29.96万 - 项目类别:
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