权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

exRNA in colorectal carcinoma: biogenesis and function

结直肠癌中的 exRNA：生物发生和功能

基本信息

批准号：
10544788
负责人：
Alissa M Weaver
金额：
$ 174.5万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-01-22 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10544788
关键词：
Address Affect Area Behavior Binding Biogenesis Biological Biological Models Biology Cell Communication Cell membrane Cell model Cell physiology Cell secretion Cells Cetuximab Communication Development Endoplasmic Reticulum Endosomes Epidermal Growth Factor Receptor Epigenetic Process Funding Gene Expression Goals Health Human Immune Intervention Intestines KRAS2 gene Large Intestine Carcinoma Ligands Lipids Malignant Epithelial Cell Malignant Neoplasms Mediating Methods MicroRNAs Modification Molecular Neoplasm Metastasis Nucleic Acids Organelles Organoids Patients Phenotype Post-Translational Protein Processing Proteins RNA RNA-Binding Proteins Repression Resistance Resource Sharing Role Signal Transduction Signaling Protein Sorting System Testing Tissues Transfer RNA United States Up-Regulation WNT Signaling Pathway Work cancer initiation cell type colonic crypt exosome extracellular extracellular vesicles inhibitor intercellular communication microvesicles novel particle programs stem cell niche synergism three dimensional cell culture trafficking tumor microenvironment tumor progression tumorigenesis

项目摘要

Summary – Overall Component Cellular communication between diverse cells is now recognized to heavily influence both cancer initiation and progression. Recently, several new forms of intercellular communication have been recognized, including exchange of proteins and RNAs via extracellular vesicles (EVs) and other carriers. Extracellular RNA (ExRNA) is particularly interesting, as it has the potential to influence gene expression and potentially epigenetic states. MicroRNAs (miRNAs) in particular have been shown to be transferred from one cell type to another to influence gene expression; however, it is clear that many kinds of exRNAs are selectively secreted from cells. What is less clear is how those exRNAs (including miRNAs) are packaged into EVs and other carriers. In addition, the overall impact of these exRNAs on cells and tissues is not yet understood. In this program, we propose to elucidate the “rules of the game” for extracellular RNA communication, using colorectal carcinoma (CRC) as a model system. Our Program will be highly synergistic, because each Project focuses on a different aspect of this problem. Thus, Project 1 will determine how subcellular contacts between organelles drive RNA and RNA-binding protein (RBP) transfer to EVs in CRC. Project 2 will identify RNA modifications and sequences that drive molecular selection of RNAs for transfer into EVs in CRC. Project 3 will identify how EVs and exomeres mediate EGFR-Wnt crosstalk in CRC. All Projects use common experimental systems, including CRC isogenic cell models, and have common biological focus (e.g. how miR-100 and miR-125b are trafficked and influence recipient cell function in CRC). Integration, synergy, and progress of the Projects will be highly enhanced by the proposed Administrative and Shared Resource Cores. Together this Program will make a major impact in the areas of CRC, tumor microenvironment, exRNA, EVs, and RNA biology.

摘要-整体组成