Light Microscopy Core

光学显微镜核心

• 批准号: 10544824
• 负责人: Lisa A. Cameron
• 金额: $ 6.26万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10544824
• 关键词: Accounting; Advisory Committees; Cancer Center Support Grant; Cells; Charge; Collection; Confocal Microscopy; Consult; Consultations; Dedications; Doctor of Philosophy; Education; Environment; Equipment; Experimental Designs; Faculty; Fees; Fluorescence; Fluorescence Microscopy; Fluorescence Recovery After Photobleaching; Funding; Future; Hour; Human Resources; Image; Image Analysis; Immunofluorescence Immunologic; Incubated; Journals; Light; Location; Malignant Neoplasms; Marketing; Medical center; Microscopy; Mission; Modality; Modeling; Mus; Optics; Photobleaching; Policies; Preparation; Productivity; Protocols documentation; Publications; Recommendation; Research; Research Institute; Research Personnel; Research Project Grants; Reservations; Resource Sharing; Resources; Sampling; Scanning; Services; Slide; Structure; Surveys; System; Technical Expertise; Techniques; Technology; Testing; Time; Tissues; Training; Universities; data acquisition; experience; fluorescence imaging; fluorescence microscope; high standard; image processing; instrument; intravital imaging; laser capture microdissection; light microscopy; live cell imaging; medical schools; medical specialties; member; microscopic imaging; model organism; multi-photon; nanosecond; new technology; novel strategies; operation; photoactivation; programs; single molecule; software systems; tissue/cell culture; ultra high resolution

ABSTRACT - LIGHT MICROSCOPY SHARED RESOURCE The Light Microscopy Shared Resource (LM) provides state-of-the-art light microscopy technology for fixed and live cells and tissues, along with scientific and technical expertise to assist in experimental design and optimal image collection. The LM shared resource offers affordable and efficient access to over $8 million in microscopic imaging resources, including confocal and conventional fluorescence instruments, multi-photon, lightsheet and super-resolution to all members of the Duke Cancer Institute (DCI). Three dedicated, full-time PhD-level staff members provide training and full technical support for all the microscopy systems. DCI members receive special priority access to equipment, training sessions and staff assistance. Hands-on individualized training and assistance with analysis is provided upon request to DCI members in advance of other users. LM is supported annually with charge backs from user fees and by the DCI CCSG, the Duke University School of Medicine and the Duke University Office of the Provost. All standard optical sectioning and fluorescence microscopy technologies are offered including confocal, resonant scanning confocal, spinning disk, live-cell imaging with several modalities, long-term incubated time-lapse, multi-photon, photoactivation/photoconversion and photokinetic (FRAP, FLIP) capabilities, and in addition, new technologies – lightsheet and super-resolution. Reservations are executed online through the university-wide software system on a first-come, first-served basis with some limits to promote efficient use. The large range of equipment with some replication of capabilities provides good capacity for users’ needs. LM’s continued priority is to serve the light microscopy needs of DCI members and all Duke researchers with support and assistance on equipment and image analysis in order to generate productivity. At the same time we keep current by demo-ing and testing new and different technologies and strive to implement new technologies that prove valuable. In 2018, LM provided services to 233 investigators, 40% of whom were DCI members, accounting for 55% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 261 publications over the project period, 71 of which were in high impact journals (IF>9), demonstrating the value of services offered by the resource.

摘要-光学显微镜共享资源 光学显微镜共享资源（LM）提供最先进的光学显微镜技术， 和活细胞和组织，沿着科学和技术专业知识，以协助实验设计， 最佳图像采集。LM共享资源提供了负担得起的和有效的访问超过800万美元， 显微成像资源，包括共焦和常规荧光仪器，多光子， 光片和超分辨率的杜克癌症研究所（DCI）的所有成员。三个专职的 博士级工作人员为所有显微镜系统提供培训和全面的技术支持。DCI成员 特别优先获得设备、培训课程和工作人员援助。亲身体验个性化 在其他用户之前，根据请求向DCI成员提供分析方面的培训和帮助。LM是 每年由用户费用和DCI CCSG，杜克大学学院支持， 医学和杜克大学教务处。所有标准光学切片和荧光 显微镜技术提供包括共聚焦，共振扫描共聚焦，旋转盘，活细胞 多种成像方式，长期孵育延时，多光子，光活化/光转化 和光动力学（FRAP，FLIP）能力，此外，新技术-光片和超分辨率。 预订是通过大学范围内的软件系统在线执行的，先到先得 并具有一些限制以促进有效使用。大范围的设备具有一定的复制能力 为用户的需求提供了良好的容量。LM的首要任务仍然是满足DCI的光学显微镜需求 成员和所有杜克研究人员在设备和图像分析方面的支持和帮助， 产生生产力。同时，我们通过演示和测试新的和不同的技术来保持最新状态 并努力实施证明有价值的新技术。 2018年，LM为233名调查人员提供服务，其中40%为DCI成员，占比55% 的总使用量，来自所有8个DCI研究项目。DCI成员使用这一共享资源有助于 在项目期间发表了261篇论文，其中71篇发表在高影响力期刊上（IF>9），证明了 资源提供的服务。