Zeiss Lightsheet Upgrade to Expand Imaging Capabilities

摘要 光片显微镜为活体完整的成像提供了明显的能力优势 生物样品和光学清除固定样品。快速三维光学剖分 光损伤使光板系统在大型器官或整体的成像方面表现出色 胚胎。我们在此应用中建议的Zeiss LightSheet Z.1升级以实现更高的折射率 指数清理解决方案，更大的样本室和更长的工作距离，更大的领域 查看目标将改变研究人员收集更大样本图像的能力，例如 作为清除整个小鼠脑或清除整个小鼠胚胎。升级的可用性 我们蔡司灯板系统的硬件和软件将增强许多项目 研究基础发育生物学和人类疾病模型。海流 仪器被安置在杜克医学院大楼内的光学显微镜核心 设施空间。升级的部件将安装到蔡司系统上，该系统已经在 运营3.5年。除了这里描述的主要和次要用户的研究之外， 系统和升级将服务于杜克大学和医学中心的整个研究基地 影响和使用将广泛存在的学校。该用户组具有以下经验 杜克大学蔡司系统的光片成像和样品制备，以及其他形式 位于其他机构。一般高水平的成像专业知识和特定的 蔡司LightSheet的经验非常适合立即有效地实施 本次升级设备为扩展蔡司光板系统的一个重要基本阵列 研究。这些拟议的升级将极大地促进新的研究能力 与NIH资助的发育生物学、神经科学和细胞生物学相关的问题 与研究声明中概述的人类疾病有关。

ABSTRACT Lightsheet microscopy offers distinct capability advantages for the imaging of live intact biological samples and optically cleared fixed samples. Rapid 3D optical sectioning with little photodamage enables lightsheet systems to excel at the imaging of large organs or whole embryos. The Zeiss Lightsheet Z.1 upgrades we propose in this application for higher refractive index clearing solutions, larger sample chambers and longer working distance, larger field of view objectives will transform researchers’ capabilities to collect images of larger samples such as cleared whole mouse brain or cleared whole mouse embryos. The availability of the upgrade hardware and software for our Zeiss lightsheet system would enhance many projects investigating fundamental developmental biology and human disease models. The current instrument is housed in a Duke School of Medicine building within the Light Microscopy Core Facility space. The upgrade parts would be installed onto to the Zeiss system which has been in operation for 3.5 years. In addition to the major and minor users’ research described here, the system and the upgrades would serve the entire research base at Duke University and Medical School where the impact and use would be widespread. The user group has experience with lightsheet imaging and sample preparation for the Zeiss system at Duke, along with other forms located at other institutions. The general high-levels of imaging expertise and specific experience with the Zeiss lightsheet are well placed to immediately and effectively implement this upgrade equipment to expand the Zeiss lightsheet system for an important array of basic research. These upgrades proposed will significantly contribute to new research capabilities related to NIH funded developmental biology, neuroscience, and cell biology questions relevant to human disorders as outlined in the research statement.