Zeiss Lightsheet Upgrade to Expand Imaging Capabilities

摘要 光片显微镜为活体完整成像提供了独特的能力优势 生物样品和光学透明的固定样品。快速3D光学切片， 光损伤使得光片系统能够在大器官或整体的成像方面表现优异 胚胎我们在此应用中提出的蔡司Lightsheet Z.1升级版可实现更高的折射率 更大的样品室和更长的工作距离，更大的 视图目标将改变研究人员收集更大样本图像的能力， 如清除的整个小鼠脑或清除的整个小鼠胚胎。升级的可用性 蔡司光片系统的硬件和软件将增强许多项目 研究基础发育生物学和人类疾病模型。当前 仪器位于光学显微镜核心内的杜克医学院大楼内 设施空间。升级部件将安装到蔡司系统上， 运行3.5年。除了这里描述的主要和次要用户的研究之外， 系统和升级将服务于整个研究基地在杜克大学和医疗 学校的影响和使用将是广泛的。用户组有经验， 杜克的蔡司系统的光片成像和样品制备，沿着还有其他形式 位于其他机构。一般高水平的成像专业知识和特定的 蔡司光片的经验可以立即有效地实施 这一升级设备，以扩大蔡司光片系统的一个重要的基本阵列， research.这些拟议的升级将大大有助于新的研究能力 与NIH资助的发育生物学、神经科学和细胞生物学相关的问题 研究报告中提到的人类疾病

ABSTRACT Lightsheet microscopy offers distinct capability advantages for the imaging of live intact biological samples and optically cleared fixed samples. Rapid 3D optical sectioning with little photodamage enables lightsheet systems to excel at the imaging of large organs or whole embryos. The Zeiss Lightsheet Z.1 upgrades we propose in this application for higher refractive index clearing solutions, larger sample chambers and longer working distance, larger field of view objectives will transform researchers’ capabilities to collect images of larger samples such as cleared whole mouse brain or cleared whole mouse embryos. The availability of the upgrade hardware and software for our Zeiss lightsheet system would enhance many projects investigating fundamental developmental biology and human disease models. The current instrument is housed in a Duke School of Medicine building within the Light Microscopy Core Facility space. The upgrade parts would be installed onto to the Zeiss system which has been in operation for 3.5 years. In addition to the major and minor users’ research described here, the system and the upgrades would serve the entire research base at Duke University and Medical School where the impact and use would be widespread. The user group has experience with lightsheet imaging and sample preparation for the Zeiss system at Duke, along with other forms located at other institutions. The general high-levels of imaging expertise and specific experience with the Zeiss lightsheet are well placed to immediately and effectively implement this upgrade equipment to expand the Zeiss lightsheet system for an important array of basic research. These upgrades proposed will significantly contribute to new research capabilities related to NIH funded developmental biology, neuroscience, and cell biology questions relevant to human disorders as outlined in the research statement.