Cannabis Vapor Exposure Impacts Reproduction

大麻蒸气暴露影响繁殖

• 批准号: 10568537
• 负责人: KANAKO HAYASHI
• 金额: $ 22.33万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10568537
• 关键词: Abnormal placentation; Adult; Adverse effects; Affect; Age; American; Apoptosis; Cannabidiol; Cannabis; Cells; Chronic; Cohort Effect; Consumption; Control Groups; DNA; DNA Damage; DNA Methylation; DNA Modification Methylases; Data; Defect; Discipline of Nursing; Drug usage; Epigenetic Process; Estrous Cycle; Ethics; Exhibits; Exposure to; Female; Fertility; First Pregnancy Trimester; Generations; Germ Cells; Health; Human; Inherited; Knowledge; Menstruation; Mus; Neonatal; Outcome; Ovary; Partner in relationship; Paternal Exposure; Pharmaceutical Preparations; Phenotype; Plants; Plasma; Population; Pregnancy; Pregnant Women; Premature Birth; Psychoses; Psychotropic Drugs; Reporting; Reproduction; Risk; Socioeconomic Factors; Sperm Count Procedure; Sperm Motility; Standardization; Surveys; Testing; Testis; Tetrahydrocannabinol; Toxicology; United States; Vagina; base; bisphenol A; design; histone methyltransferase; histone modification; in utero; insight; male; marijuana legalization; marijuana use; marijuana user; methylome; offspring; postnatal; pregnant; reproductive; reproductive function; steroid hormone; stillbirth; transcriptome; transmission process; vapor

Project Summary Cannabis is considered the most widely used psychoactive drug in the United States and all over the world. In 2020, the National Survey on Drug Use and Health (NSDUH) has reported that over 48 million Americans (~16% of the population) ages 12 and older used cannabis in the past year. This number has increased due to the expanding legalization of cannabis consumption in the US. Higher potency strains of cannabis plants have emerged over the past two decades. Unsurprisingly, adverse health outcomes associated with cannabis consumption have also been increasing. The proposed study will provide the first impact, which is the transgenerational effects of in utero and nursing cannabis vapor exposure on phenotypic defects of male and female reproductive functions. Because cannabis is a psychoactive drug, chronic or frequent use of cannabis is associated with a variety of adverse health outcomes. However, little is known about its effects on reproduction, especially the long-term consequences of cannabis on offspring and subsequent generations. To date, no systematic studies examining reproductive phenotypic defects and their associated mechanisms caused by cannabis have been performed. In addition, it is entirely unknown whether the adverse effects of cannabis on reproduction are sustained via germline transmission to subsequent generations. Therefore, this application proposes as a first step to examine the transgenerational effects of cannabis vapor exposure on male and female reproductive functions. We will characterize how cannabis vapor exposure during pregnancy and nursing affects male and female reproductive parameters in neonatal and adult testes and ovaries to understand paternally or maternally transmitted alterations in male or female germ cells. Because at least 7% of pregnant women use cannabis throughout pregnancy (~12% during the first trimester and 5.3% daily users), an appreciation of the impact of cannabis use on reproductive functions in subsequent offspring is crucial.

项目摘要 大麻被认为是美国和世界各地使用最广泛的精神活性药物。在……里面 2020年，全国药物使用与健康调查(NSDUH)报告称，超过4800万美国人 (约占人口的16%)12岁及以上的人在过去一年中使用大麻。这一数字有所增加，原因是 美国不断扩大的大麻消费合法化。效力较高的大麻植物菌株 在过去的二十年里出现了。毫不奇怪，与大麻有关的不良健康后果 消费也一直在增加。拟议的研究将提供第一个影响，即 宫内和护理大麻蒸气暴露对男性和女性表型缺陷的跨代影响 女性的生殖功能。由于大麻是一种精神活性药物，长期或频繁使用大麻 与各种不利的健康后果有关。然而，人们对它对以下方面的影响知之甚少 生殖，特别是大麻对后代和后代的长期后果。至 到目前为止，还没有系统研究生殖表型缺陷及其相关机制 已经进行了大麻致死事件的调查。此外，完全不清楚的是， 大麻繁殖是通过生殖系传播给后代来维持的。因此，这 应用建议作为第一步检查大麻蒸气暴露对 男性和女性的生殖功能。我们将描述怀孕期间大麻蒸气暴露的特征 而护理影响新生儿和成人睾丸和卵巢的男性和女性生殖参数，以 了解男性或女性生殖细胞通过父系或母系传播的变化。因为至少有7% 在整个怀孕期间使用大麻的孕妇比例(怀孕前三个月约12%，每日吸食比例5.3%)， 认识到大麻使用对后代生殖功能的影响至关重要。