Cannabis Vapor Exposure Impacts Reproduction

项目摘要 大麻被认为是美国和世界各地使用最广泛的精神活性药物。在 2020年，全国药物使用和健康调查（NSDUH）报告称，超过4800万美国人 （约占人口的16%）年龄在12岁及以上的人在过去一年中使用大麻。这一数字的增加是由于 美国大麻消费合法化的扩大。更高效力的大麻植物株具有 在过去的二十年里出现了。毫不奇怪，与大麻相关的不良健康后果 消费也在增加。拟议的研究将产生第一个影响，即 子宫内和哺乳期大麻蒸汽暴露对男性和女性表型缺陷的跨代影响 女性生殖功能。由于大麻是一种精神活性药物，长期或频繁使用大麻 与多种不良健康后果有关。然而，人们对它的影响知之甚少。 特别是大麻对后代和子孙后代的长期影响。到 迄今为止，没有系统的研究检查生殖表型缺陷及其相关机制 由大麻引起的疾病此外，完全不知道是否有不利影响， 大麻对生殖的影响是通过生殖细胞传递给后代来维持的。因此本 申请提出作为第一步，检查大麻蒸汽暴露对后代的影响， 男性和女性的生殖功能。我们将描述如何在怀孕期间大麻蒸汽暴露 哺乳影响新生儿和成年睾丸和卵巢中的男性和女性生殖参数， 了解男性或女性生殖细胞中父亲或母亲传递的改变。因为至少有7% 的孕妇在整个怀孕期间使用大麻（约12%在头三个月，5.3%每天使用）， 认识到使用大麻对后代生殖功能的影响至关重要。

Project Summary Cannabis is considered the most widely used psychoactive drug in the United States and all over the world. In 2020, the National Survey on Drug Use and Health (NSDUH) has reported that over 48 million Americans (~16% of the population) ages 12 and older used cannabis in the past year. This number has increased due to the expanding legalization of cannabis consumption in the US. Higher potency strains of cannabis plants have emerged over the past two decades. Unsurprisingly, adverse health outcomes associated with cannabis consumption have also been increasing. The proposed study will provide the first impact, which is the transgenerational effects of in utero and nursing cannabis vapor exposure on phenotypic defects of male and female reproductive functions. Because cannabis is a psychoactive drug, chronic or frequent use of cannabis is associated with a variety of adverse health outcomes. However, little is known about its effects on reproduction, especially the long-term consequences of cannabis on offspring and subsequent generations. To date, no systematic studies examining reproductive phenotypic defects and their associated mechanisms caused by cannabis have been performed. In addition, it is entirely unknown whether the adverse effects of cannabis on reproduction are sustained via germline transmission to subsequent generations. Therefore, this application proposes as a first step to examine the transgenerational effects of cannabis vapor exposure on male and female reproductive functions. We will characterize how cannabis vapor exposure during pregnancy and nursing affects male and female reproductive parameters in neonatal and adult testes and ovaries to understand paternally or maternally transmitted alterations in male or female germ cells. Because at least 7% of pregnant women use cannabis throughout pregnancy (~12% during the first trimester and 5.3% daily users), an appreciation of the impact of cannabis use on reproductive functions in subsequent offspring is crucial.