Biomarker and Analytical Chemistry Core

生物标志物和分析化学核心

• 批准号: 10665901
• 负责人: JOHN P RICHIE
• 金额: $ 113.71万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-30 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10665901
• 关键词: 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol; 8-hydroxy-2' -deoxyguanosine; Acetylcysteine; Aerosols; Analytical Chemistry; Animals; Archives; Benzo(a)pyrene; Biological; Biological Markers; Blood; Cells; Characteristics; Chemicals; Clinical; Clinical Chemistry; Clinical Research; Collaborations; Collection; Communities; DNA Adduction; DNA Adducts; Data Analyses; Development; Disease; Educational Activities; Educational process of instructing; Electronic cigarette; Emerging Tobacco Products; Exhalation; Glutathione; High Pressure Liquid Chromatography; Human; Human Resources; IL6 gene; Inflammation; Isoprostanes; Laboratories; Laboratory Animal Models; Length; Maintenance; Mass Fragmentography; Measurement; Measures; Mentorship; Methodology; Methods; Mission; Modeling; Nicotine; Oral mucous membrane structure; Organic Chemistry; Oxidation-Reduction; Pattern; Phospholipids; Physical assessment; Physical condensation; Pilot Projects; Play; Postdoctoral Fellow; Preparation; Protocols documentation; Research; Research Design; Research Personnel; Research Support; Resources; Role; Saliva; Sampling; Science; Smoke; Smoking; Stable Isotope Labeling; Standardization; TNF gene; Techniques; Time; Tobacco; Tobacco smoke; Tobacco use; Toxic effect; Training; Urine; Weight; Work; addiction; analytical method; biological specimen archives; carcinogenicity; career; chemical property; clinical investigation; design; electronic liquid; instrumentation; investigator training; method development; novel; physical property; tobacco products; tobacco regulatory science; tobacco user; vaping; ventilation

Project Summery / Abstract: Biomarker and Analytical Chemistry Core The PSU TCORS includes the three major interdisciplinary projects, pilot projects and educational activities that will rely heavily on the use of analytical laboratory measurements for the assessment of tobacco products and biomarkers analyses for the assessment of the effects of tobacco-related exposures on tobacco product users and laboratory animal models of tobacco related harm. To support these activities, the Biomarkers and Analytical Chemistry Core (BAC) will play a pivotal role. The BAC was established in 2013 as part of our original TCORS and, since that time, has continued to support the Center activities by providing expertise for study design, sample processing and analyses of tobacco products and related biomarkers, methods development and data interpretation. We plan to continue with these activities to meet the needs of the research to be conducted in the main and pilot project of the current proposal. As such, the BAC focuses on the use of analytical chemistry and clinical chemistry methodologies supported by a strong expertise in synthetic organic chemistry. The BAC activities will include the use of standardized analytical techniques and instrumentation as well as the development of new analytical methods and design of novel instrumentation for use with new and emerging tobacco products. In addition to supporting the research mission, the BAC will also play a key role in the educational activities of the Center in collaboration with the Career Enhancement Core by providing mentorship and training for TCORS postdoctoral scholars and teaching a 3-credit course on the use of tobacco biomarkers (PHS 684 Biomarkers in Tobacco Research). The BAC will support the three major projects, pilot projects, and educational activities by focusing on 8 specific aims. These include (1) measurement of toxic and carcinogenic agents in tobacco products and their resulting smoke/aerosols; (2) maintenance of experimental smoking laboratories with the capability of machine smoking/vaping a wide variety of tobacco products for chemical analysis or animal exposure studies; (3) quantification of biomarkers in biological samples from animal or clinical studies; (4) assessment of physical and chemical characteristics of tobacco products; (5) preparation of synthetic standards required for chemical analyses; (6) processing and archival of biological samples; (7) development of new methods and instrumentation for assessing tobacco- derived agents in new and emerging products; and (8) training of TCORS investigators on the measurement and use of biomarkers and their interpretation from both a research and regulatory perspective.

项目总结/摘要：生物标志物和分析化学核心 PSU TCORS包括三个主要的跨学科项目，试点项目和教育活动 这将严重依赖于使用分析实验室测量来评估烟草产品， 评估烟草相关暴露对烟草制品影响的生物标志物分析 吸烟者和实验室动物模型的烟草相关危害。为了支持这些活动，生物标志物和 分析化学核心（BAC）将发挥关键作用。BAC成立于2013年，是我们 最初的TCORS，并从那时起，继续支持中心的活动，提供专业知识， 研究设计、样品处理和烟草制品及相关生物标志物的分析、方法 开发和数据解释。我们计划继续进行这些活动，以满足 将在本提案的主要和试点项目中进行的研究。因此，BAC侧重于 使用分析化学和临床化学方法，并获得以下方面的强大专业知识 合成有机化学BAC活动将包括使用标准化分析技术， 仪器以及新的分析方法的开发和新仪器的设计， 使用新的和新兴的烟草产品。除了支持研究使命，BAC还将 与职业发展核心合作，在中心的教育活动中发挥关键作用， 为TCORS博士后学者提供指导和培训，并教授关于使用的3学分课程 烟草生物标志物（PHS 684烟草研究生物标志物）。BAC将支持三大 项目、试点项目和教育活动，重点是8个具体目标。其中包括（1） 测量烟草制品及其产生的烟雾/气溶胶中的有毒和致癌物质;（2） 维护实验吸烟实验室，具有广泛的机器吸烟/vaping能力 用于化学分析或动物暴露研究的各种烟草产品;（3） 来自动物或临床研究的生物样品;（4）评估 烟草制品;（5）化学分析所需的合成标准的制备;（6）加工和 生物样品的存档;（7）开发评估烟草的新方法和仪器- 新产品和新兴产品中的衍生物;（8）培训TCORS研究人员进行测量 以及生物标志物的使用及其从研究和监管角度的解释。