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Patterned, Stimulus-Independent Neuronal Activity In the Developing Drosophila Visual System: Origin and Contribution to Synaptic Maturation

果蝇视觉系统发育中的模式化、刺激独立的神经元活动：起源和对突触成熟的贡献

基本信息

批准号：
10665676
负责人：
Orkun Akin
金额：
$ 37.96万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-01 至 2026-07-31
项目状态：
未结题

项目摘要

Project Summary/Abstract Synaptic connections determine how neural circuits process information. Understanding how the strength and specificity of these connections is established is a central challenge in neurobiology. In many parts of the developing mammalian brain, stereotyped patterns of stimulus-independent neuronal activity precede sensory- driven responses. Whether and how this developmental activity guides synapse assembly at the level of defined cell types and circuits is not well-understood. Here, much of the challenge is due to the size and complexity of the mammalian brain itself: Even in the retina, where developmental activity is best characterized, the technical barriers to pursuing synapse level questions are significant. We recently discovered analogous patterned, stimulus-independent neural activity (PSINA, pronounced `see-nah') in the developing Drosophila brain. With the ever-growing knowledge of its neurobiology, spanning the connectome to behavior, the fly is unmatched in its promise for cell type- and circuit- level studies. PSINA is globally coordinated with brain-wide, periodic active and silent phases. In the visual system, each cell type participates in PSINA with distinct and stereotyped spatio-temporal patterns of activity. These developmental activity patterns are correlated between pairs of neurons known to be synaptic partners in the adult. Our long term goal is to test the hypothesis that the cell-type-specific activity patterns of PSINA refine the emerging connectome to generate wild-type synaptic strength and specificity. Here, we will work toward this goal by leveraging a new genetic handle on PSINA: Trpγ, a cation channel with a weak preference for Ca2+, is required for wild-type PSINA. In trpγ mutants, the amplitude of activity is reduced by >50% across the whole brain, and cell-type-specific activity patterns and synapse numbers are altered. Trpγ is expressed in <1.5% of the neurons in the brain. Notably, silencing only these neurons by overexpressing a hyperpolarizing channel attenuates PSINA by >90%. This indicates that some or all of this diverse group of ~1,700 Trpγ-expressing (i.e. Trpγ+) neurons are critical to coordinating PSINA in the developing brain. We hypothesize that Trpγ+ neurons are the source of the cell-type-specific activity patterns. In Aim 1, we will identify individual Trpγ+ neurons that innervate the visual system and test if these neurons specify the activity patterns of their post-synaptic partners. Determining the origin of these patterns will allow us to ask whether they are the cause or consequence of synapse and circuit maturation. In Aim 2, we will focus on a specific neuron that is part of the well-studied motion detection circuit and ask if the strength of its post-synaptic contacts are altered in trpγ mutants. Identifying the cellular origin of the activity patterns and understanding the effect of PSINA on synaptic development will allow us to reversibly silence, alter, or possibly re-program PSINA. With this knowledge, we will be able to define the contribution of developmental activity to the structural and functional maturation of synapses and circuits, to sensory processing, to learning, memory, and behavior.

项目总结/文摘