Characterizing the preterm infant skin microbiome and microbial shifts that precede neonatal late onset sepsis (LOS)

描述早产儿皮肤微生物组的特征以及新生儿迟发性败血症 (LOS) 之前的微生物变化

• 批准号: 10664071
• 负责人: Jennifer Jane Schoch
• 金额: $ 16.16万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10664071
• 关键词: 16S ribosomal RNA sequencing; Address; Affect; Antibiotics; Antisepsis; Award; Bacteria; Bathing; Bioinformatics; Birth; Candida; Caring; Catheters; Cesarean section; Childhood; Clinical; Clinical Trials; Collection; Control Groups; Corynebacterium; Cutaneous; Data; Dermatology; Development; Disease; Early identification; Environment; Ethnic Origin; Evolution; Exhibits; Failure; Florida; Future; Genus staphylococcus; Gestational Age; Goals; Hospitalization; Hospitals; Humidity; Incidence; Infant; Intervention; K-Series Research Career Programs; Klebsiella; Knowledge; Laboratories; Lactobacillus; Lead; Leadership; Learning; Life; Medicine; Mentors; Metagenomics; Methods; Microbe; Natural History; Neonatal; Outcome; Outpatients; Parents; Pathogenicity; Physicians; Predisposition; Premature Infant; Prevention; Prevention strategy; Race; Research; Research Activity; Research Design; Resources; Risk; Role; Sample Size; Sampling; Science; Scientist; Sepsis; Serratia; Shotguns; Site; Skin; Streptococcus; Swab; Testing; Therapeutic; Training; Training Activity; Training Programs; Translational Research; Universities; Very Low Birth Weight Infant; Work; career; career development; career networking; cohort; college; design; experience; extreme prematurity; feeding; gut microbes; indexing; innovation; insight; large datasets; late onset sepsis; metagenomic sequencing; microbial; microbiome; microbiome composition; microbiome research; microbiota transplantation; mortality; neonatal sepsis; novel; opportunistic pathogen; pathogen; pathogen exposure; pathogenic bacteria; perinatal complications; preterm newborn; programs; recruit; sex; skills; skin barrier; skin microbiome; skin microbiota; skin organogenesis; statistics; surveillance strategy; vaginal microbiota

PROJECT SUMMARY Despite recent emphasis on the microbiome, there are few skin microbiome studies in infants; even fewer have been performed in preterm infants. Currently, there is a significant gap in our knowledge of the normal evolution of the preterm infant skin microbiome from birth to an independent, site-specific cutaneous microbiome. Preliminary studies show that preterm infants have limited microbial diversity and different predominant cutaneous microbes compared to full-term infants. This lack of a robust, diverse skin microbiome may render preterm infants susceptible to pathogenic skin bacteria that cause disease, particularly neonatal sepsis. The research goals of this mentored career-development award are to: 1) characterize the establishment of the neonatal skin microbiome in a cohort of preterm infants from birth to 4 weeks and compare to demographic and clinical variables, 2) recruit a cohort of term infants to determine if the preterm infant skin microbiome is significantly different than term infants, and 3) analyze cultured pathogens with the skin microbiome in late-onset neonatal sepsis (LOS). Our methods represent a departure from the currently available studies, which provide opportunistic sampling of the neonatal skin microbiome rather than sampling at defined timepoints. This study will vertically-advance the field by sampling the preterm skin microbiome systematically after birth. The role of the skin microbiome in neonatal sepsis has also not been described, and the design of this study will provide a better understanding of changes in the skin microbiome before late onset sepsis. Together, these aims will provide insight into the normal and abnormal development of the preterm infant skin microbiome. Future studies will then leverage the neonatal skin microbiome via therapeutic and preventative strategies to lower the incidence of LOS. This K23 is the next logical step in the training program started under my KL2 award, and the final step in my transition to an independent physician scientist. This proposal describes mentored research and training activities designed to launch a novel neonatal skin microbiome research program, the first in the field of pediatric dermatology. Five training and career development goals, facilitated by my mentoring team, are required to accomplish this. These include: extend my understanding of research design, deepen my knowledge of microbiome and relevant laboratory science, gain working knowledge of statistics/bioinformatics (including advanced metagenomic analyses), learn to lead an independent laboratory, and expand my professional network within microbiome science. The University of Florida provides a rich environment with expertise in the neonatal microbiome and metagenomics, ideal to extend my previous work as I develop a novel neonatal skin microbiome research program. The resources at UF will be augmented by additional skin microbiome expertise and laboratory resources from Dr. Pammi at Baylor College of Medicine and Diversigen Inc.

项目摘要 尽管最近强调了微生物组，但婴儿的皮肤微生物组很少。甚至更少 是在早产儿中进行的。目前，我们对正常进化的了解存在很大的差距 从出生到独立的，特异性的皮肤微生物组的早产婴儿皮肤微生物组的。 初步研究表明，早产儿的微生物多样性有限和不同 皮肤微生物与完整的婴儿相比。这种缺乏强大的，多样化的皮肤微生物组可能会呈现 早产婴儿容易受到引起疾病的致病性皮肤细菌，尤其是新生儿败血症。这 这个指导职业发展奖的研究目标是：1）特征是建立 从出生到4周的早产儿中的新生儿皮肤微生物组，并将 临床变量，2）招募一组术语婴儿，以确定早产婴儿皮肤微生物组是否为 与术语婴儿明显不同，3）与晚期的皮肤微生物组分析培养的病原体 新生儿败血症（LOS）。我们的方法代表了与当前可用的研究的背离 新生儿皮肤微生物组的机会抽样，而不是在定义的时间点上进行采样。这项研究会 出生后系统地对早产皮肤微生物组采样，从垂直促进该领域。角色 新生儿败血症中的皮肤微生物组也尚未描述，这项研究的设计将提供更好的 了解败血症之前皮肤微生物组的变化。这些目标将共同提供 深入了解早产婴儿皮肤微生物组的正常和异常发育。然后将来的研究将 通过治疗和预防策略来利用新生儿皮肤微生物组，以降低LOS的发生率。 这个K23是培训计划的下一个逻辑步骤，是根据我的KL2奖开始的，也是我的最后一步 过渡到独立的医师科学家。该建议描述了指导的研究和培训活动 旨在启动一种新型的新生儿皮肤微生物组研究计划，该计划是小儿领域的首次 皮肤科。由我的指导团队促进的五个培训和职业发展目标必须 做到这一点。这些包括：扩展我对研究设计的理解，加深我对 微生物组和相关实验室科学，获得统计/生物信息学的工作知识（包括 高级宏基因组分析），学会领导独立实验室并扩展我的专业网络 在微生物组科学中。佛罗里达大学提供了丰富的环境，具有新生儿的专业知识 微生物组和宏基因组学，非常适合扩展我以前的工作，因为我开发了新的新生儿皮肤微生物组 研究计划。 UF的资源将通过其他皮肤微生物组专业知识和 贝勒医学院和多元化学院帕米博士的实验室资源