权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Staphylococcal Biofilm and Disease

葡萄球菌生物膜和疾病

基本信息

批准号：
10665017
负责人：
KENNETH W. BAYLES
金额：
$ 233.35万
依托单位：
UNIVERSITY OF NEBRASKA MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10665017
关键词：
Abscess Acetates Amino Acids Anti-Inflammatory Agents Automobile Driving Biology Carbon Catabolism Cells Clinical Management Communities Development Developmental Process Disease Education Environment Enzymes Funding Genetic Transcription Genome Genus staphylococcus Glucose Goals Growth Healthcare Systems Histone Deacetylase Inhibitor Hyaluronidase Immune Immune response Infection Inflammatory Innate Immune Response Interleukin-10 Kinetics Knowledge Libraries Life Cycle Stages Life Style Ligands Macrophage Medical Device Metabolic Metabolic Pathway Metabolism Microbial Biofilms Microfluidics Modeling Mus Mutation Nebraska Nitrogen Nutrient availability Online Systems Peptide Hydrolases Peptide Transport Peptides Phosphotransferases Polymers Production Property Protocols documentation Regulation Research Role Scientific Inquiry Source Staphylococcus aureus System Testing Tissues Virulence Factors Vision Work bioimaging extracellular fitness improved in vivo in vivo imaging system inflammatory milieu insight metabolome metabolomics mutant novel strategies nuclease online resource pathogen permissiveness programs proline permease receptor reflectance confocal microscopy synergism tool web page web site

项目摘要

During the previous funding cycle, our program project entitled “Staphylococcal biofilm and disease” has employed in-depth mechanistic approaches to define the developmental and metabolic processes important in Staphylococcus aureus biofilm development. A key aspect of the synergy of this project is that the knowledge gained was used as context to provide a more detailed understanding of the acquisition of available nutrients within specific host niches, as well as the impact of biofilm growth on the host immune response. These studies have led to a greatly enhanced understanding of the way in which S. aureus adapts to a host environment, providing new fundamental insight into biofilm development and novel approaches to the clinical management of staphylococcal disease. The overall hypothesis driving the goals of the proposed program project, S. aureus biofilm development creates unique metabolic niches that promote an immune suppressive environment, is a natural outgrowth of the current funding cycle and is tested in four synergistic and complementary projects that encompass a broad spectrum of synergistic and highly collaborative activities ranging from the basic biology of biofilm development and matrix regulation, to the host-associated metabolic processes that influence the immune response. To support the efforts of these four projects, we propose a continuation of our Bioimaging Core that maintains a BioFlux microfluidics system for biofilm growth and analysis, confocal microscopy, and an In Vivo Imaging System (IVIS). In addition, we propose a new Metabolomics Core that will establish and maintain the protocols and modeling needed to support the four projects associated with this PPG. Importantly, our vision is that the work of this core will lead to the development of a web-based metabolomics tool (funded through a separate mechanism) that will serve not only as an education tool to enhance the overall understanding of the S. aureus metabolome, but also as a hypothesis generator in support of scientific inquiry. Once this tool is established and validated, we will then make it available to the entire staphylococcal research community as a web-based resource that is integrated with our existing Nebraska Transposon Mutant Library (NTML) website. Finally, we propose an Administrative Core that will provide the administrative support needed to maximize the interactions between project leaders and to ensure that their projects maintain optimal synergy.

在上一个资助周期，我们的项目题为“葡萄球菌生物膜和疾病”，采用了深入的机制方法来定义重要的发育和代谢过程，金黄色葡萄球菌生物膜形成。该项目协同作用的一个关键方面是， gained被用作背景，以提供对有效养分获取的更详细理解在特定的宿主生态位内，以及生物膜生长对宿主免疫反应的影响。这些研究使人们对S.金黄色葡萄球菌适应宿主环境，为生物膜的形成和临床管理提供了新的基本见解葡萄球菌病总体假设驱动的目标，拟议的计划项目，S。金黄色生物膜的形成创造了独特的代谢生态位，环境，是当前供资周期的自然产物，并在四个协同和互补项目，包括广泛的协同和高度合作的活动从生物膜发育和基质调节的基础生物学，到宿主相关的代谢，影响免疫反应的过程。为支持这四项计划，我们建议延续我们的生物成像核心，维持生物膜生长的BioFlux微流体系统，分析、共聚焦显微镜和体内成像系统（IVIS）。此外，我们还提出了一个新的代谢组学核心，将建立和维护支持四个与此PPG相关的项目。重要的是，我们的愿景是，这个核心的工作将导致发展一个基于网络的代谢组学工具（通过一个单独的机制资助），不仅将作为一个教育工具，以提高对S。金黄色葡萄球菌代谢组，但也作为一个假设，支持科学研究的发电机。一旦这个工具建立和验证，我们将提供它作为基于网络的资源，与我们现有的内布拉斯加州转座子突变库（NTML）网站。最后，我们提出了一个行政核心，提供所需的行政支持，以最大限度地提高项目领导人之间的互动，并确保他们的项目保持最佳的协同作用。

项目成果

期刊论文数量（127）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Host autophagy is exploited by the intracellular parasite Toxoplasma gondii to enhance amino acids levels.

细胞内寄生虫弓形虫利用宿主自噬来提高氨基酸水平。

DOI：
10.1101/2023.12.08.570852
发表时间：
2023
期刊：
bioRxiv : the preprint server for biology
影响因子：
0
作者：
White,MatthewD;Angara,RajendraK;Dias,LeticiaTorres;Shinde,DhananjayD;Thomas,VinaiC;Augusto,Leonardo
通讯作者：
Augusto,Leonardo

Processing, Export, and Identification of Novel Linear Peptides from Staphylococcus aureus.

金黄色葡萄球菌新型线性肽的加工、出口和鉴定。