Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qToF) Mass Spectrometer

总结 高分辨率和高灵敏度是现代生物医学研究的一个重要方面 由最先进的质谱（MS）仪器提供的质量分析。Lumigen仪器 中心（LIC）在韦恩州立大学有一个使命，以提供研究人员在大学和在 通过可靠和多样化的基于用户的MS分析，为区域提供服务，以提高其研究生产力。作为 LIC质谱实验室战略计划的一部分，质谱咨询 委员会确定了用户目前面临的两个挑战。首先，一个17岁的孩子 液相色谱-飞行时间（LC-TOF）仪器阻止了重要的高分辨率质量 分析，这极大地影响了用户研究的生产力。第二，过时的硬件和 14岁的LTQ Orbitrap XL的软件已经停止了对各种项目的应用。最后，发展 由于缺乏新的和有效的方法， 强大的技术，包括离子迁移谱（IMS）。 为了应对这些挑战，本申请要求资金用于离子迁移谱仪-四极杆 飞行时间（IMS-qTOF）质谱仪在韦恩州立大学。该工具将显著 提升本校在质谱学方面的研究成果。首先，IMS-qTOF将 更换过时和故障的MS仪器，用于小分子的常规高分辨率分析， 生物分子其次，该仪器将提供校园共享设施中唯一的质谱仪 具有IMS能力，这将使NIH资助的研究人员能够扩展和转变他们的研究 活动该仪器的多种功能将包括自上而下和自下而上的蛋白质 鉴定、蛋白质加合物和缀合表征、蛋白质构象研究、生物标志物 发现、药物-抗体表征、精确质量测定和多组学工作流程。的 该仪器将支持韦恩州立大学和 区域区域，其中包括化学家，生物学家，环境科学家和医学研究人员。的 IMS-qTOF将在Lumigen仪器中心（LIC）进行安装和维护， 来自大学的机构支持，研究副总裁办公室，和学院 文科和理科。LIC在仪器管理方面有着堪称典范的记录，拥有敬业的工作人员， 设施和管理，以支持仪器的长期使用。重要的是，以用户为中心 LIC的结构将促进为每个用户开发一系列独特的新方法 研究项目。收购IMS-qToF符合Mass的长期战略计划 LIC的光谱实验室，旨在提供最先进和可靠的MS仪器， 在韦恩州立大学和当地社区实施创新用户发起的项目。

Summary Biomedical research in the modern age has been revolutionized by the high resolution and high sensitivity mass analysis provided by state-of-the-art mass spectrometry (MS) instruments. The Lumigen Instrument Center (LIC) at Wayne State University has a mission to provide researchers at the university and in the regional area with reliable and diverse user-based MS analysis to elevate their research productivity. As part of the strategic plan of the Mass Spectrometry Laboratory in the LIC, the Mass Spectrometry Advisory Committee identified two current challenges for users. First, the substantial down time of a 17-year old Liquid Chromatography-Time-of-Flight (LC-TOF) instrument has prevented vital high resolution mass analysis, which has dramatically affected user research productivity. Second, the outdated hardware and software of a 14 year-old LTQ Orbitrap XL has stalled application to variety of projects. Finally, development of new methods to address the unique needs of individual users is limited due to the absence of new and powerful technologies, including Ion Mobility Spectrometry (IMS). To address these challenges, this application requests funds for an Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qTOF) Mass Spectrometer at Wayne State University. The instrument will significantly enhance research accomplishment in mass spectrometry (MS) on our campus. First, the IMS-qTOF will replace outdated and failing MS instruments for routine high-resolution analysis of small molecules and biomolecules. Second, the instrument will provide the only mass spectrometer in a shared facility on campus with IMS capability, which will enable NIH-funded researchers to expand and transform their research activities. The multiple capabilities of the instrument will include Top-Down and Bottom-Up Protein identification, protein adduct and conjugation characterization, protein conformational studies, biomarker discovery, drug-antibody characterization, accurate mass determination, and multi-omics workflows. The instrument will support the research programs of at least 19 users at Wayne State University and the regional area, which include chemists, biologists, environmental scientists, and medical researchers. The IMS-qTOF will be housed and maintained in the Lumigen Instrument Center (LIC), which has strong institution support from the University, the Office of the Vice President of Research, and the College of Liberal Arts and Science. The LIC has an exemplary record of instrument stewardship, with dedicated staff, facilities, and management to support the long-term use of the instrument. Importantly, the user-focused structure of the LIC will promote development of a unique array of new methods for each user’s individual research projects. The acquisition of an IMS-qToF is aligned with the long-term strategic plan of the Mass Spectrometry Laboratory at the LIC, which seeks to provide state-of-the-art and reliable MS instruments to implement innovative user-initiated projects at Wayne State University and local area community.