Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qToF) Mass Spectrometer

离子淌度光谱仪 - 四极杆飞行时间 (IMS-qToF) 质谱仪

• 批准号: 10630627
• 负责人: Mary Kay H Pflum
• 金额: $ 68.5万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630627
• 关键词: 14 year old; 17 year old; Address; Advisory Committees; Affect; Age; Antibodies; Area; Arts; Biomedical Research; Communities; Computer software; Dedications; Development; Funding; Individual; Institution; Laboratories; Liquid Chromatography; Mass Spectrum Analysis; Medical; Methods; Mission; Modernization; Pharmaceutical Preparations; Productivity; Protein Conformation; Proteins; Request for Applications; Research; Research Activity; Research Personnel; Research Project Grants; Research Support; Resolution; Science; Scientist; Spectrometry; Strategic Planning; Structure; Technology; Time; United States National Institutes of Health; Universities; adduct; biomarker discovery; college; innovation; instrument; ion mobility; mass spectrometer; multiple omics; prevent; programs; small molecule

Summary Biomedical research in the modern age has been revolutionized by the high resolution and high sensitivity mass analysis provided by state-of-the-art mass spectrometry (MS) instruments. The Lumigen Instrument Center (LIC) at Wayne State University has a mission to provide researchers at the university and in the regional area with reliable and diverse user-based MS analysis to elevate their research productivity. As part of the strategic plan of the Mass Spectrometry Laboratory in the LIC, the Mass Spectrometry Advisory Committee identified two current challenges for users. First, the substantial down time of a 17-year old Liquid Chromatography-Time-of-Flight (LC-TOF) instrument has prevented vital high resolution mass analysis, which has dramatically affected user research productivity. Second, the outdated hardware and software of a 14 year-old LTQ Orbitrap XL has stalled application to variety of projects. Finally, development of new methods to address the unique needs of individual users is limited due to the absence of new and powerful technologies, including Ion Mobility Spectrometry (IMS). To address these challenges, this application requests funds for an Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qTOF) Mass Spectrometer at Wayne State University. The instrument will significantly enhance research accomplishment in mass spectrometry (MS) on our campus. First, the IMS-qTOF will replace outdated and failing MS instruments for routine high-resolution analysis of small molecules and biomolecules. Second, the instrument will provide the only mass spectrometer in a shared facility on campus with IMS capability, which will enable NIH-funded researchers to expand and transform their research activities. The multiple capabilities of the instrument will include Top-Down and Bottom-Up Protein identification, protein adduct and conjugation characterization, protein conformational studies, biomarker discovery, drug-antibody characterization, accurate mass determination, and multi-omics workflows. The instrument will support the research programs of at least 19 users at Wayne State University and the regional area, which include chemists, biologists, environmental scientists, and medical researchers. The IMS-qTOF will be housed and maintained in the Lumigen Instrument Center (LIC), which has strong institution support from the University, the Office of the Vice President of Research, and the College of Liberal Arts and Science. The LIC has an exemplary record of instrument stewardship, with dedicated staff, facilities, and management to support the long-term use of the instrument. Importantly, the user-focused structure of the LIC will promote development of a unique array of new methods for each user’s individual research projects. The acquisition of an IMS-qToF is aligned with the long-term strategic plan of the Mass Spectrometry Laboratory at the LIC, which seeks to provide state-of-the-art and reliable MS instruments to implement innovative user-initiated projects at Wayne State University and local area community.

摘要 现代的生物医学研究已经因为高分辨率和高灵敏度而发生了革命性的变化 由最先进的质谱仪(MS)提供的质量分析。卢米根乐器 韦恩州立大学中心(LIC)的使命是为该大学和 为区域区域提供可靠和多样化的基于用户的MS分析，以提高其研究生产率。AS LIC的质谱学实验室战略计划的一部分，质谱学咨询 委员会确定了用户目前面临的两个挑战。首先，一个17岁少年的大量停机时间 液相色谱飞行时间(LC-TOF)仪器已经阻止了至关重要的高分辨率质量 分析，这极大地影响了用户研究的效率。第二，过时的硬件和 已有14年历史的LTQ Orbitrap XL的软件在各种项目中的应用陷入停滞。最后，发展 解决个人用户独特需求的新方法由于缺乏新的和 强大的技术，包括离子迁移率光谱(IMS)。 为了应对这些挑战，该应用程序请求为离子迁移率光谱分析-四极杆提供资金 韦恩州立大学的飞行时间(IMS-qTOF)质谱仪。该仪器将显著地 提高我校在质谱学(MS)方面的研究成果。首先，IMS-qTOF将 更换过时和故障的MS仪器，以进行常规的小分子和高分辨率分析 生物分子。其次，该仪器将在校园内的共享设施中提供唯一的质谱仪 具有IMS能力，这将使NIH资助的研究人员能够扩展和转变他们的研究 活动。该仪器的多种功能将包括自上而下和自下而上的蛋白质 鉴定，蛋白质加合物和连接特性，蛋白质构象研究，生物标记物 发现、药物抗体表征、准确的质量测定和多组学工作流程。这个 该仪器将支持至少19个用户在韦恩州立大学和 区域，包括化学家、生物学家、环境科学家和医学研究人员。这个 IMS-qTOF将安置在Lumigen仪器中心(LIC)并进行维护，该中心拥有强大的 来自大学、副校长办公室和学院的机构支持 文科和理科。LIC拥有堪称典范的仪器管理记录，拥有敬业的员工， 支持仪器长期使用的设施和管理。重要的是，以用户为中心 LIC的结构将促进针对每个用户个人的独特的新方法阵列的开发 研究项目。收购IMS-qToF符合MASS的长期战略计划 LIC的光谱实验室，该实验室致力于为 在韦恩州立大学和当地社区实施由用户发起的创新项目。