Nikon Multiphoton (MP) Imaging System

尼康多光子 (MP) 成像系统

• 批准号: 10632942
• 负责人: JAMES E CASSAT
• 金额: $ 85.72万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-15 至 2024-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632942
• 关键词: Anesthesia procedures; Biological Process; Biology; Cells; Communicable Diseases; Contract Services; Core Facility; Dedications; Diabetes Mellitus; Digestive System Disorders; Ensure; Epithelium; Equipment; Event; Financial Support; Funding; Human; Image; Immunology; Infection; Inflammation; Institution; Lasers; Link; Malignant Neoplasms; Mass Spectrum Analysis; Microscope; Microscopy; Neurobiology; Operative Surgical Procedures; Organism; Output; Perfusion; Preparation; Recording of previous events; Research; Research Personnel; Resource Sharing; Resources; Science; Specialist; Specimen; Speed; Subcellular structure; System; Tissue imaging; Tissues; Training; United States National Institutes of Health; Visualization; Wages; Whole Organism; animal imaging; biomedical imaging; cellular imaging; ex vivo imaging; imaging system; in vivo; instrument; intravital imaging; light microscopy; lung injury; molecular imaging; multi-photon; multiphoton imaging; operation

PROJECT SUMMARY / ABSTRACT Funds are requested to purchase a Nikon A1R Multiphoton (MP) Imaging System, to be integrated into the Vanderbilt Cell Imaging Shared Resource (CISR). Vanderbilt has a history of excellence in biomedical imaging, with outstanding facilities for human and live animal imaging within the Institute of Imaging Sciences, groundbreaking molecular imaging within the Mass Spectrometry Research Center, and cutting-edge equipment for advanced light microscopy within the CISR. However, the ability to integrate these outstanding resources to image biologic processes across all scales – i.e. from the whole organism down to subcellular structure – has been limited by the lack of an imaging solution to bridge live animal imaging to ex vivo imaging of tissues and cells. The proposed MP System is capable of visualizing cellular events deep within the tissues of living organisms through intravital imaging, providing a critical link between in vivo and ex vivo scales. A group of 12 NIH-funded User Groups across Vanderbilt will initially be supported by this instrument. These investigators have unmet imaging needs to support NIH-funded projects spanning infectious diseases, immunology, neurobiology, digestive diseases, epithelial biology, diabetes, lung injury, and cancer. The proposed configuration of the MP System is based on the needs of the User Groups and includes: 1) a dual- output laser with primary tunable beam (800-1300 nm) and secondary fixed (1040 nm) beam to support imaging depth of up to 1.4mm, 2) a high-speed resonant scanner for dynamic imaging of up to 720fps, 3) a fully automated, large-format stage and upright microscope stand to accommodate anesthesia and intravital preparations, and 4) a selection of objectives specifically chosen to support the proposed User projects. Additionally, with support from the Vanderbilt Institute for Infection, Immunology, and Inflammation (VI4), we have already established a dedicated biosafety level 2 imaging suite with ancillary anesthesia, surgical, and perfusion equipment to house the MP System and ensure that we can accommodate biohazardous specimens. Vanderbilt is providing financial support to promote the long-term operation of the instrument, including salary for technical staff and necessary service contracts. Technical support and training of new users will be facilitated by the exceptional staff within the CISR, which has a greater than 20-year history of assisting over 300 labs on campus. Moreover, the Nikon Center of Excellence housed within the CISR provides a dedicated local team of specialists to support the microscopy needs of investigators on campus. Collectively, the proposed MP System, together with outstanding institutional and core facility support, will enable the long-term objective of establishing intravital imaging at Vanderbilt.

项目总结/摘要 申请资金用于购买尼康A1 R多光子（MP）成像系统，该系统将集成到 范德比尔特细胞成像共享资源（CISR）。范德比尔特在生物医学成像方面有着卓越的历史， 在成像科学研究所内拥有出色的人类和活体动物成像设施， 质谱研究中心内的开创性分子成像，以及尖端设备 先进的光学显微镜。然而，整合这些优秀资源的能力， 对所有尺度的生物过程进行成像--即从整个生物体到亚细胞结构-- 由于缺乏将活体动物成像与组织的离体成像连接起来的成像解决方案， 细胞所提出的MP系统能够可视化活体组织深处的细胞事件， 生物体通过活体成像，提供了体内和体外规模之间的关键环节。一组12 NIH资助的范德比尔特用户组最初将由该仪器支持。这些调查人员 未满足的成像需求，以支持NIH资助的项目，涵盖传染病，免疫学，神经生物学， 消化系统疾病、上皮生物学、糖尿病、肺损伤和癌症。 MP系统的拟议配置是基于用户组的需求，包括：1）一个双- 输出激光器，具有初级可调谐光束（800-1300 nm）和次级固定光束（1040 nm）以支持成像 深度可达1.4mm，2）用于动态成像的高速共振扫描器，高达720 fps，3）完全 自动化、大幅面载物台和直立显微镜支架，可容纳麻醉和活体 准备工作，以及4）一个目标的选择，专门选择支持拟议的用户项目。 此外，在范德比尔特感染、免疫学和炎症研究所（VI 4）的支持下，我们 我已经建立了一个专门的生物安全2级成像室，配有辅助麻醉、手术和 灌注设备，以容纳MP系统，并确保我们可以容纳生物危害性标本。 范德比尔特正在提供财政支持，以促进该仪器的长期运行，包括工资 技术人员和必要的服务合同。将为新用户的技术支持和培训提供便利 由CISR内部的优秀工作人员提供，CISR拥有超过20年的历史，为300多个实验室提供帮助， 校园此外，CISR内的尼康卓越中心提供了一个专门的本地团队， 专家来支持校园内研究人员的显微镜需求。总的来说，拟议的MP系统， 加上出色的机构和核心设施支持，将实现建立 在范德比尔特做活体成像