Expanding regional capacity for single molecule sequencing through the purchase of the Sequel IIe sequencing system

项目摘要 路易斯维尔大学的测序技术中心（STC）正在请求支持，以获得太平洋 生物科学Sequel IIe测序系统。所要求的系统将是第二个续集IIe系统， 该中心，根据不同STC用户群的需求，包括区域，国家和国际 由校外资助的调查人员远远超过了我们最初的单一仪器的能力。自推出以来 2021年，STC协助规划、准备和执行了许多项目， 长读段、单分子、实时（SMRT）测序，用于使用标准高保真（HiFi） 工作流程，以及那些涉及开发定制，创新的靶向方法，以阐明复杂的 具有定相的、高度准确的长读段的基因组基因座或社区。与其他测序系统不同， 在市场上，Sequel IIe系统具有长读取能力（150 bp - 100 kb）和最高的 任何NGS和单分子测序仪器均可提供的准确度（范围从Q30，99.9%到 Q60，99.9999%，在我们的项目），这两个都是至关重要的，以满足我们的主要，次要， 服务用户。STC已经建立，并已具备基础设施和业务能力， 实施这一要求的第二个续集IIe系统，这是绝对必要的，以满足我们的需要， 不断增长的用户群，主要用户研究项目，以及使用该电源的进一步基因组学方法开发 SMRT测序技术。路易斯维尔大学，特别是研究和创新办公室， 致力于通过对STC的财务和基础设施投资来支持所请求的系统。 从新的参考基因组的产生到病毒储库和免疫位点的靶向表征， 在整个肯塔基州，STC的SARS-CoV-2病原体监测至关重要 该设施已经并将利用其在SMRT测序和拟议的新Sequel IIe系统方面的专业知识， 与当地和国家专家密切合作，促进各种各样的尖端基因组学项目， 研究领域的主要目标是促进和激励研究和创新，以支持增长 路易斯维尔大学的研究机构。

PROJECT SUMMARY The University of Louisville’s Sequencing Technology Center (STC) is requesting support to acquire a Pacific Biosciences Sequel IIe sequencing system. The requested system would be the second Sequel IIe system in the Center, as demand from the diverse STC user base, comprised of regional, national, and international extramurally funded investigators has far exceeded the capacity of our initial, single instrument. Since launching in 2021, the STC has assisted in the planning, preparation, and execution of many projects leveraging the power of long read, single molecule, real-time (SMRT) sequencing for projects that use both standard high fidelity (HiFi) workflows, and those that involve development of custom, innovative targeted methods to elucidate complex genomic loci or communities with phased, highly accurate long reads. Unlike other sequencing systems on the market, the Sequel IIe system has the combination of long read capabilities (150 bp – 100 kb) and the highest accuracy available from any NGS- and single molecule-sequencing instrument (ranging from Q30, 99.9% - to Q60, 99.9999%, across our projects), both of which are critical for meeting the needs of our Major, Minor, and service users. The STC is well established and already has the infrastructure and operational capabilities to implement this requested second Sequel IIe system, which is absolutely necessary for meeting the needs of our growing user base, Major User research projects, and further genomics methods development using the power of SMRT sequencing technology. The University of Louisville, particularly the Office of Research and Innovation, is dedicated to supporting the requested system through financial and infrastructure investment into the STC. From the generation of new reference genomes to targeted characterization of viral reservoirs and immune loci, and critically important SARS-CoV-2 pathogen surveillance across the Commonwealth of Kentucky, the STC facility has and will use its expertise with SMRT sequencing and the proposed new Sequel IIe system to work closely with local and national experts to facilitate cutting-edge genomics projects across a wide variety of research areas with the major goal of promoting and inspiring research and innovation in support of the growth of the research enterprise at the University of Louisville.