IsoSpark single cell proteomic and transcriptomic system for Keck Microarray Shared Resource (KMSR)

用于 Keck 微阵列共享资源 (KMSR) 的 IsoSpark 单细胞蛋白质组和转录组系统

• 批准号: 10632845
• 负责人: Guilin Wang
• 金额: $ 13.87万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632845
• 关键词: Acute Renal Failure with Renal Papillary Necrosis; Aging; Autoimmune Diabetes; Bar Codes; Basic Science; Biomedical Research; COVID-19; Capital; Cell Separation; Cell physiology; Cells; Chronic Kidney Failure; Communities; DNA; Data; Development; Ensure; Equipment; Funding; Genomics; Glioma; Immune; Infrastructure; Injury to Kidney; Institution; Investments; Malignant neoplasm of pancreas; Messenger RNA; Methods; Microarray Shared Resource; Nerve Degeneration; Parkinson Disease; Production; Productivity; Proteins; Proteomics; Research Personnel; Resolution; Services; System; Talents; Techniques; Technology; United States National Institutes of Health; Universities; Viral; base; cell type; cost effective; experience; fundamental research; gene therapy; insight; instrumentation; multiple omics; next generation; novel; single-cell RNA sequencing; transcriptomics; tumor microenvironment

Project Summary/Abstract Keck Microarray Shared Resource (KMSR) has been providing cutting-edge next-generation genomics technologies to support the fundamental research needs of hundreds of Yale and non-Yale NIH-funded investigators. Most recently, with the advances in single-cell isolation and barcoding technologies, the field of single cell genomics/proteomics and multiomics has started offering unprecedented opportunities to profile DNA, mRNA, and proteins at a single-cell resolution. Since 2016, KMSR has been successfully providing a variety of single cell services, most of them using 10x genomics technology and focusing on Single-cell RNA sequencing (scRNA-seq), which has been used to gain insights into cell types and states. However, this technique only provided one facet with low correlation to true cellular function. New analysis methods that combine scRNA-seq with proteomic profiling has been in active development. This would further reveal unique insights and give a better understanding of single-cell function. The requested IsoSpark system is a unique single-cell platform enables highly multiplexed proteomic and transcriptomic analysis. With the newly developed Duomic chip, IsoSpark is becoming the first platform integrating single-cell functional proteomics with transcriptomics, providing unprecedented insights into the relation between transcriptomics and proteomics. Twenty-seven investigators from 4 institutions support our request for funding of IsoSpark system, with apt justifications. They propose to use the requested system to study novel viral based immune-gene therapy, immune dysregulation in COVID-19, Parkinson’s disease, Tumor Microenvironment, autoimmune diabetes, Cerebellar Neurodegeneration, aging, glioma, pancreatic cancer, and Immune Dysregulation during Transition from Acute Kidney Injury (AKI) to chronic kidney disease (CKD). Moreover, the requested equipment is easy to use and cost-effective and has sufficient throughput to support the projects cited in this application and beyond. The strengths of this proposal include; (1) an efficient and effective centralized facility serving an extremely diverse and productive investigator user base; (2) the extensive experience of the PI (3) the demonstrated ability of KMSR to effectively integrate cutting edge data production and analysis for the benefit of hundreds of Yale and non-Yale researchers, and (4) the extensive infrastructure and expertise that is available to bring the requested instrumentation on-line and to oversee its continuous use. Yale University has made a major investment in capital and institutional talent to build the world-class genomic center that has proven highly successful. The requested instrumentation will leverage the infrastructure to ensure its high value and broad impact on NIH-funded biomedical and basic research within and beyond the Yale community.

项目总结/文摘