IsoSpark single cell proteomic and transcriptomic system for Keck Microarray Shared Resource (KMSR)

用于 Keck 微阵列共享资源 (KMSR) 的 IsoSpark 单细胞蛋白质组和转录组系统

• 批准号: 10632845
• 负责人: Guilin Wang
• 金额: $ 13.87万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632845
• 关键词: Acute Renal Failure with Renal Papillary Necrosis; Aging; Autoimmune Diabetes; Bar Codes; Basic Science; Biomedical Research; COVID-19; Capital; Cell Separation; Cell physiology; Cells; Chronic Kidney Failure; Communities; DNA; Data; Development; Ensure; Equipment; Funding; Genomics; Glioma; Immune; Infrastructure; Injury to Kidney; Institution; Investments; Malignant neoplasm of pancreas; Messenger RNA; Methods; Microarray Shared Resource; Nerve Degeneration; Parkinson Disease; Production; Productivity; Proteins; Proteomics; Research Personnel; Resolution; Services; System; Talents; Techniques; Technology; United States National Institutes of Health; Universities; Viral; base; cell type; cost effective; experience; fundamental research; gene therapy; insight; instrumentation; multiple omics; next generation; novel; single-cell RNA sequencing; transcriptomics; tumor microenvironment

Project Summary/Abstract Keck Microarray Shared Resource (KMSR) has been providing cutting-edge next-generation genomics technologies to support the fundamental research needs of hundreds of Yale and non-Yale NIH-funded investigators. Most recently, with the advances in single-cell isolation and barcoding technologies, the field of single cell genomics/proteomics and multiomics has started offering unprecedented opportunities to profile DNA, mRNA, and proteins at a single-cell resolution. Since 2016, KMSR has been successfully providing a variety of single cell services, most of them using 10x genomics technology and focusing on Single-cell RNA sequencing (scRNA-seq), which has been used to gain insights into cell types and states. However, this technique only provided one facet with low correlation to true cellular function. New analysis methods that combine scRNA-seq with proteomic profiling has been in active development. This would further reveal unique insights and give a better understanding of single-cell function. The requested IsoSpark system is a unique single-cell platform enables highly multiplexed proteomic and transcriptomic analysis. With the newly developed Duomic chip, IsoSpark is becoming the first platform integrating single-cell functional proteomics with transcriptomics, providing unprecedented insights into the relation between transcriptomics and proteomics. Twenty-seven investigators from 4 institutions support our request for funding of IsoSpark system, with apt justifications. They propose to use the requested system to study novel viral based immune-gene therapy, immune dysregulation in COVID-19, Parkinson’s disease, Tumor Microenvironment, autoimmune diabetes, Cerebellar Neurodegeneration, aging, glioma, pancreatic cancer, and Immune Dysregulation during Transition from Acute Kidney Injury (AKI) to chronic kidney disease (CKD). Moreover, the requested equipment is easy to use and cost-effective and has sufficient throughput to support the projects cited in this application and beyond. The strengths of this proposal include; (1) an efficient and effective centralized facility serving an extremely diverse and productive investigator user base; (2) the extensive experience of the PI (3) the demonstrated ability of KMSR to effectively integrate cutting edge data production and analysis for the benefit of hundreds of Yale and non-Yale researchers, and (4) the extensive infrastructure and expertise that is available to bring the requested instrumentation on-line and to oversee its continuous use. Yale University has made a major investment in capital and institutional talent to build the world-class genomic center that has proven highly successful. The requested instrumentation will leverage the infrastructure to ensure its high value and broad impact on NIH-funded biomedical and basic research within and beyond the Yale community.

项目摘要/摘要 Keck微阵列共享资源(KMSR)一直在提供尖端的下一代基因组学 支持数百名耶鲁大学和非耶鲁国家卫生研究院资助的基础研究需求的技术 调查人员。最近，随着单细胞分离和条形码技术的进步， 单细胞基因组学/蛋白质组学和多重组学已经开始提供前所未有的机会来分析DNA， 单细胞分辨率的信使核糖核酸和蛋白质。自2016年以来，KMSR成功地提供了各种 单细胞服务，其中大多数使用10倍基因组技术，专注于单细胞RNA测序 (scRNA-seq)，它已被用于了解细胞类型和状态。然而，这项技术仅 提供了一个与真实细胞功能相关性较低的方面。结合scRNA-seq的新分析方法 随着蛋白质组学研究的不断深入，蛋白质组学的研究也在不断深入。这将进一步揭示独特的见解，并提供 对单细胞功能有更好的理解。 所要求的IsoSpark系统是一个独特的单细胞平台，能够实现高度多元化的蛋白质组和 转录分析。凭借最新开发的双芯片，IsoSpark正在成为第一个平台 将单细胞功能蛋白质组学与转录组学相结合，提供了对 转录组学与蛋白质组学的关系。 来自4个机构的27名调查人员支持我们为IsoSpark系统提供资金的请求，APT 辩解。他们建议使用所要求的系统来研究新的基于病毒的免疫基因疗法， 新冠肺炎免疫失调，帕金森氏病，肿瘤微环境，自身免疫性糖尿病， 小脑神经退行性变、衰老、胶质瘤、胰腺癌和过渡期免疫调节失调 从急性肾脏损伤(AKI)到慢性肾脏疾病(CKD)。 此外，所要求的设备使用方便、成本效益高，并有足够的生产能力来支持 本申请书及以后引用的项目。这项建议的优点包括：(1)有效率和 高效的集中式设施，为极为多样化和富有成效的调查员用户群提供服务；(2)广泛的 PI的经验(3)KMSR有效集成尖端数据生产的能力 并对数百名耶鲁和非耶鲁研究人员的利益进行分析，以及(4)广泛的基础设施 以及可用来使所请求的仪器上线并监督其持续使用的专业知识。 耶鲁大学在资本和机构人才方面进行了重大投资，以建立世界级的基因组 该中心已被证明非常成功。所请求的检测将利用基础设施来确保 它对NIH资助的耶鲁内外的生物医学和基础研究具有很高的价值和广泛的影响 社区。