Supplement for temporal bone histological tissue scanning equipment

颞骨组织学组织扫描设备补充品

• 批准号: 10671303
• 负责人: Andrew J. Oxenham
• 金额: $ 22.15万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-03-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10671303
• 关键词: Accidents; Acoustics; Affect; Age; Archives; Auditory; Auditory Perception; Behavioral; Bone Tissue; Clinical; Clinical Research; Cochlea; Cochlear Implants; Cochlear Nerve; Collection; Communities; Data; Databases; Distal; Equipment; Funding Opportunities; Future; Goals; Hair Cells; Hearing; Hearing Aids; Histologic; Housing; Human; Image; Institution; Laboratories; Labyrinth; National Institute on Deafness and Other Communication Disorders; Natural Disasters; Nerve Fibers; Neurons; Organ; Parents; Pathologic; Patients; Performance; Property; Registries; Research; Research Personnel; Resolution; Resources; Scanning; Slide; Specimen; Speech Perception; Stria Vascularis; Temporal bone structure; Tissues; Universities; biological specimen archives; digital; digital repositories; hearing impairment; high resolution imaging; improved; interest; meetings; middle ear; neuroimaging; parent grant; parent project; relating to nervous system; searchable database; sex; spiral ganglion

Project summary This proposal is for the purchase of temporal bone histological tissue scanning equipment as a supplement to the parent proposal, R01 DC012262, entitled “Spectro-temporal interactions in electric and acoustic auditory perception”. The long-term goal of the parent proposal is to improve auditory and speech perception via cochlear implants (CIs) and hearing aids, through a greater understanding of the basic mechanisms that contribute to, and limit, the perception of speech in challenging acoustic conditions. The long-term goals of the parent project will benefit greatly from the use of otopathological specimens. Our university is fortunate to have one of the three largest human temporal bone laboratories in the US, housing a well-diversified collection of 2,252 human temporal bones. Our plan is to use digitized human temporal bone slides to relate the behavioral and neuroimaging results gathered under the parent grant with otopathological data, providing a unique opportunity to relate our perceptual findings to the cellular level within the inner ear and thereby assess the pathological underpinnings of altered speech perception. Our plan is to search the database of our otopathology laboratory and select specimens of special interest to our research (from patients who have similar background as ones in our clinical study: age- and sex- matched, with and without hearing loss). These specimens will be scanned using a high-resolution, high-throughput tissue scanner, which will be acquired through this funding opportunity (Zeiss Axioscan 7). We will then scrutinize the middle and inner ears of temporal bones for properties that relate to the perceptual performance of patients with CIs and hearing aids. We will perform quantitative analysis of cochlear hair cells, stria vascularis, spiral ganglion neurons, and dendritic distal cochlear nerve fibers. The benefits of having digital versions of the archival temporal bones will extend far beyond the parent project and beyond researchers at our institution, as these digital versions will protect the information from these irreplaceable specimens from being damaged in the case of accidents or natural disasters. In addition, by making the digitized collection of archival specimens widely available, they will become an invaluable long-term resource to the wider clinical and scientific communities involved with auditory and temporal bone research, meeting one of the fundamental objectives of the NIDCD Registry. The images will ultimately be made available to the scientific community through NIDCD approved databases.

项目摘要 本建议书是为购买颞骨组织学组织扫描设备作为补充， 父提案R 01 DC 012262，题为“电和声听觉中的频谱-时间相互作用 知觉”。家长提案的长期目标是通过耳蜗改善听觉和言语感知 植入物（CI）和助听器，通过更好地了解有助于， 并限制在具有挑战性的声学条件下对语音的感知。父项目的长期目标 会从耳病理学标本的使用中受益匪浅我们大学很幸运拥有这三所大学之一 美国最大的人类颞骨实验室，拥有2，252名人类颞骨标本， 颞骨我们的计划是使用数字化的人类颞骨切片将行为和 神经影像学结果收集下的父母赠款与耳病理学数据，提供了一个独特的机会， 将我们的感知发现与内耳内的细胞水平联系起来，从而评估病理性 言语感知改变的基础我们的计划是搜索我们耳病理学实验室的数据库 并选择对我们的研究特别感兴趣的标本（来自与 我们的临床研究：年龄和性别匹配，有和没有听力损失）。这些标本将被扫描 使用高分辨率，高通量组织扫描仪，这将通过这次资助机会获得 （Zeiss Axioscan 7）。然后，我们将仔细检查颞骨的中耳和内耳， 对CI和助听器患者的感知表现。我们将进行定量分析， 耳蜗毛细胞、血管纹、螺旋神经节神经元和树突状远端耳蜗神经纤维。的 拥有数字化版本的颞骨档案的好处将远远超出母项目， 除了我们机构的研究人员之外，因为这些数字版本将保护这些信息不受 不可替代的标本在事故或自然灾害的情况下被损坏。此外，通过使 随着档案标本的数字化收藏广泛使用，它们将成为宝贵的长期资源， 更广泛的临床和科学界参与听觉和颞骨研究，会议之一， NIDCD登记处的基本目标。这些图像最终将提供给 科学界通过NIDCD批准的数据库。