Supplement for temporal bone histological tissue scanning equipment

项目总结 这项提议是为了购买颞骨组织扫描设备，作为对 父提案R01DC012262，题为《电声听觉中的频谱-时间相互作用》 感知力“。家长提案的长期目标是通过耳蜗改善听觉和言语知觉 植入物(CI)和助听器，通过更好地了解有助于 以及限制，在具有挑战性的声学条件下对语音的感知。母项目的长期目标 将极大地受益于耳病理标本的使用。我们大学很幸运地拥有这三所学校之一 美国最大的人类颞骨实验室，拥有2252名人类 颞骨。我们的计划是使用数字化的人类颞骨幻灯片来联系行为和 在父母资助下收集的神经成像结果和耳部病理数据，提供了一个独特的机会 将我们的感知发现与内耳内的细胞水平联系起来，从而评估病理 改变言语知觉的基础。我们的计划是搜索我们耳部病理实验室的数据库 并选择对我们的研究特别感兴趣的样本(来自与 我们的临床研究：年龄和性别匹配，有或没有听力损失)。这些标本将被扫描 使用高分辨率、高通量的组织扫描仪，这将通过这次融资机会获得 (蔡司Axioscan 7)。然后，我们将仔细检查颞骨的中耳和内耳，以寻找与 对配戴顺式耳机和助听器患者的知觉表现的影响。我们将进行定量分析， 耳蜗毛细胞、血管纹、螺旋神经节神经元和耳蜗远端树突神经纤维。这个 拥有档案颞骨的数字版本的好处将远远超出父项目和 超越了我们机构的研究人员，因为这些数字版本将保护信息不受 不可替代的标本，在发生事故或自然灾害时不会被损坏。此外，通过制作 档案标本的数字化收集随处可见，它们将成为无价的长期资源 为更广泛的临床和科学界参与听觉和颞骨研究，满足一个 NIDCD登记处的基本目标。这些图像最终将提供给 科学界通过NIDCD批准的数据库。

Project summary This proposal is for the purchase of temporal bone histological tissue scanning equipment as a supplement to the parent proposal, R01 DC012262, entitled “Spectro-temporal interactions in electric and acoustic auditory perception”. The long-term goal of the parent proposal is to improve auditory and speech perception via cochlear implants (CIs) and hearing aids, through a greater understanding of the basic mechanisms that contribute to, and limit, the perception of speech in challenging acoustic conditions. The long-term goals of the parent project will benefit greatly from the use of otopathological specimens. Our university is fortunate to have one of the three largest human temporal bone laboratories in the US, housing a well-diversified collection of 2,252 human temporal bones. Our plan is to use digitized human temporal bone slides to relate the behavioral and neuroimaging results gathered under the parent grant with otopathological data, providing a unique opportunity to relate our perceptual findings to the cellular level within the inner ear and thereby assess the pathological underpinnings of altered speech perception. Our plan is to search the database of our otopathology laboratory and select specimens of special interest to our research (from patients who have similar background as ones in our clinical study: age- and sex- matched, with and without hearing loss). These specimens will be scanned using a high-resolution, high-throughput tissue scanner, which will be acquired through this funding opportunity (Zeiss Axioscan 7). We will then scrutinize the middle and inner ears of temporal bones for properties that relate to the perceptual performance of patients with CIs and hearing aids. We will perform quantitative analysis of cochlear hair cells, stria vascularis, spiral ganglion neurons, and dendritic distal cochlear nerve fibers. The benefits of having digital versions of the archival temporal bones will extend far beyond the parent project and beyond researchers at our institution, as these digital versions will protect the information from these irreplaceable specimens from being damaged in the case of accidents or natural disasters. In addition, by making the digitized collection of archival specimens widely available, they will become an invaluable long-term resource to the wider clinical and scientific communities involved with auditory and temporal bone research, meeting one of the fundamental objectives of the NIDCD Registry. The images will ultimately be made available to the scientific community through NIDCD approved databases.