Component: Husbandry and Management Core

组成部分：畜牧和管理核心

• 批准号: 10666556
• 负责人: Kamm Prongay
• 金额: $ 141.29万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10666556
• 关键词: AIDS-Related Opportunistic Infections; Acquired Immunodeficiency Syndrome; Animal Model; Animals; Artificial Insemination; Breeding; Caring; Cytomegalovirus; Derivation procedure; Development; Fostering; Future; HIV; HIV/AIDS; Health; Herpesvirus 1; Human; Infant; Lymphocryptovirus; Macaca; Macaca mulatta; Maintenance; Measles; Monitor; Mothers; Partner in relationship; Pathogenesis; Population; Practice Management; Research; Resources; Rhadinovirus; Rhesus; SIV; Shelter facility; Simian Foamy Virus; Simian Retroviruses; Testing; Time; Vaccines; Viral Vector; Virus; animal resource; experience; germ free condition; improved; innovation; novel; programs; social

PROJECT SUMMARY, Core A: Husbandry and Management Core Expanded SPF (eSPF) rhesus macaques (Macaca mulatta), which are defined as being negative to rhesus rhadinovirus, simian immunodeficiency virus, simian foamy virus, simian T-lymphotropic virus, simian retrovirus, macacine herpesvirus-1 (BV), measles, and rhesus cytomegalovirus, are necessary to study an increasing range of viruses important to human health, as well as the development and testing of novel viral vectors. eSPF macaques have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, and the development of HIV treatments and vaccines. Currently, the national need for genetically characterized eSPF rhesus macaques for HIV/AIDS research far exceeds the current availability. Therefore it is imperative that the available eSPF rhesus macaque populations grow in size. The successful maintenance of expanded SPF colonies is challenging, however, and requires strict management practices in order to remain specific pathogen free. With over 18 years’ experience in establishing and maintaining a colony of eSPF animals, the ONPRC is the leader at managing eSPF rhesus macaques. The overall aims of the Husbandry and Management Core are to breed and grow the ONPRC eSPF colony in order to meet existing and future animal resource demands, continue to provide and improve upon the existing exemplary eSPF husbandry and veterinary care, and to improve the eSPF resource by making the entire colony lymphocryptovirus negative. To do this we will provide innovative team based management practices including expansion of the colony into outdoor sheltered housing, introduction of a time- mated breeding program and artificial insemination, a foster mother program for abandoned infants, implementation of new enrichment and social monitoring strategies, and re-derivation of LCV negative infants from within the eSPF colony.

项目总结，核心A：畜牧业和管理核心 扩展SPF(ESPF)猕猴(Macaca Mulatta)，定义为恒河猴阴性 杆状病毒、猴免疫缺陷病毒、猴泡沫病毒、猴T淋巴细胞病毒、猴 逆转录病毒、猕猴疱疹病毒-1(BV)、麻疹和恒河猴巨细胞病毒是研究 对人类健康至关重要的病毒种类不断增加，以及新型病毒的开发和测试 向量。ESPF猕猴已经成为艾滋病毒/艾滋病研究的主要动物模型，包括对 艾滋病毒的发病机制，艾滋病相关的机会性感染，以及艾滋病毒治疗和疫苗的发展。 目前，全国需要对ESPF恒河猴的基因特征进行艾滋病毒/艾滋病的研究 超出当前可用范围。因此，现有的ESPF恒河猴是当务之急 种群规模不断扩大。然而，成功维持扩大的SPF菌落是具有挑战性的， 并要求严格的管理做法，以保持特定的病原体。有超过18年的历史 在建立和维护ESPF动物群体方面的经验，ONPRC在管理方面处于领先地位 ESPF恒河猴。畜牧经营核心的总体目标是培育和培育 ONPRC ESPF群体为满足现有和未来的动物资源需求，继续提供和 改进现有的示范ESPF畜牧和兽医护理，并改善ESPF资源 通过使整个菌落的淋巴分泌病毒呈阴性。为此，我们将提供以创新团队为基础的 管理做法，包括将殖民地扩展到户外庇护房屋，引入时间- 交配繁殖计划和人工授精计划，这是一项针对被遗弃婴儿的养母计划， 实施新的充实和社会监测战略，并重新衍生LCV阴性婴儿 来自ESPF菌落内部。