Component: Husbandry and Management Core

组成部分：畜牧和管理核心

• 批准号: 10014301
• 负责人: Kamm Prongay
• 金额: $ 141.29万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10014301
• 关键词: AIDS-Related Opportunistic Infections; AIDS/HIV problem; Acquired Immunodeficiency Syndrome; Animal Model; Animals; Artificial Insemination; Breeding; Caring; Cytomegalovirus; Derivation procedure; Development; Fostering; Future; HIV; Health; Herpesvirus 1; Housing; Human; Infant; Lymphocryptovirus; Macaca; Macaca mulatta; Maintenance; Measles; Monitor; Mothers; Partner in relationship; Pathogenesis; Population; Practice Management; Research; Resources; Rhadinovirus; Rhesus; SIV; Simian Foamy Virus; Simian Retroviruses; Testing; Time; Vaccines; Viral Vector; Virus; animal resource; base; experience; germ free condition; improved; innovation; novel; programs; social

PROJECT SUMMARY, Core A: Husbandry and Management Core Expanded SPF (eSPF) rhesus macaques (Macaca mulatta), which are defined as being negative to rhesus rhadinovirus, simian immunodeficiency virus, simian foamy virus, simian T-lymphotropic virus, simian retrovirus, macacine herpesvirus-1 (BV), measles, and rhesus cytomegalovirus, are necessary to study an increasing range of viruses important to human health, as well as the development and testing of novel viral vectors. eSPF macaques have become the premier animal model for HIV/AIDS research, including studies on HIV pathogenesis, AIDS-related opportunistic infections, and the development of HIV treatments and vaccines. Currently, the national need for genetically characterized eSPF rhesus macaques for HIV/AIDS research far exceeds the current availability. Therefore it is imperative that the available eSPF rhesus macaque populations grow in size. The successful maintenance of expanded SPF colonies is challenging, however, and requires strict management practices in order to remain specific pathogen free. With over 18 years’ experience in establishing and maintaining a colony of eSPF animals, the ONPRC is the leader at managing eSPF rhesus macaques. The overall aims of the Husbandry and Management Core are to breed and grow the ONPRC eSPF colony in order to meet existing and future animal resource demands, continue to provide and improve upon the existing exemplary eSPF husbandry and veterinary care, and to improve the eSPF resource by making the entire colony lymphocryptovirus negative. To do this we will provide innovative team based management practices including expansion of the colony into outdoor sheltered housing, introduction of a time- mated breeding program and artificial insemination, a foster mother program for abandoned infants, implementation of new enrichment and social monitoring strategies, and re-derivation of LCV negative infants from within the eSPF colony.

项目总结，核心A：饲养和管理核心 扩展SPF（eSPF）恒河猴（Macaca mulatta），定义为恒河猴阴性 猴嗜T淋巴细胞病毒 逆转录病毒，猕猴疱疹病毒-1（BV），麻疹和恒河猴巨细胞病毒，是研究 增加对人类健康重要的病毒的种类，以及开发和测试新的病毒 向量。eSPF猕猴已成为艾滋病毒/艾滋病研究的首要动物模型， 艾滋病发病机制，艾滋病相关的机会性感染，以及艾滋病治疗和疫苗的发展。 目前，国家对用于艾滋病毒/艾滋病研究的具有遗传特征的eSPF恒河猴的需求远远超过了 超过了目前的可用性。因此，必须将可用的SPF恒河猴 人口规模增长。然而，成功维持扩大的SPF菌落是具有挑战性的， 并且需要严格的管理实践以保持无特定病原体。拥有超过18年的 在建立和维护一个eSPF动物群体的经验，ONPRC是管理的领导者 SPF恒河猴。畜牧和管理核心的总体目标是培育和发展 为了满足现有和未来的动物资源需求，ONPRC eSPF菌落继续提供和 改进现有的示范性eSPF畜牧业和兽医护理，并改善eSPF资源 使整个菌落的淋巴隐病毒呈阴性为此，我们将提供基于创新团队的 管理措施，包括将殖民地扩展为户外庇护所，引入时间- 交配繁殖计划和人工授精，弃婴寄养计划， 实施新的丰富和社会监测策略，并重新推导LCV阴性婴儿 在ESPF的殖民地内。