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Animal Core Facility

动物核心设施

基本信息

批准号：
10666695
负责人：
John Curlee Wilkinson
金额：
$ 59.72万
依托单位：
NORTH DAKOTA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-01 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10666695
关键词：
Achievement Address Advisory Committees Animal Cancer Model Animal Experimentation Animal Experiments Animal Housing Animal Model Animals Apoptosis Area Biological Models Biomedical Research Breeding Cancer Etiology Carcinogenesis Mechanism Cell Communication Centers of Research Excellence Cessation of life Clinical Clinical Management Collaborations Communities Complement Complex Core Facility Development Diagnosis Diagnostic Disease Early Diagnosis Early treatment Elements Ensure Experimental Designs Extramural Activities Faculty Faculty Recruitment Fluorouracil Fostering Genetic Engineering Genetically Engineered Mouse Goals Histocytochemistry Histology Housing IACUC Immunocompromised Host Immunohistochemistry Individual Institution Invaded KPC model KRASG12D Leucovorin Longevity Maintenance Malignant Neoplasms Malignant neoplasm of pancreas Mentors Methods Mission Modeling Mouse Strains Mus National Cancer Institute National Institute of Diabetes and Digestive and Kidney Diseases National Institute of General Medical Sciences Neoplasm Metastasis Pancreatic Ductal Adenocarcinoma Patients Phase Pilot Projects Play Postdoctoral Fellow Productivity Regimen Regulation Research Research Infrastructure Research Personnel Resources Role Sampling Scientist Senior Scientist Services Site Solid Survival Rate Testing Therapeutic Tissues Toxic effect Training United States United States National Institutes of Health animal care anticancer research biobank cancer diagnosis cancer therapy career cell type chemotherapeutic agent efficacy study fluorescence imaging gemcitabine graduate student imaging approach imaging modality implantation improved in vivo irinotecan model development novel novel therapeutic intervention novel therapeutics oxaliplatin patient derived xenograft model programs protocol development standard of care subcutaneous translational study treatment response treatment strategy tumor working group

项目摘要

Project Summary/Abstract (Animal Core Facility) Animal models play a vital role in the establishment and refinement of improved therapies for various cancers, including pancreatic ductal adenocarcinoma (PDAC). Thus, when the NDSU Center for Diagnostic and Therapeutic Strategies in Pancreatic Cancer was established, a key component included was the Animal Research Core Facility. During Phase I of this project, the Animal Core has made significant contributions to the COBRE project's mission and the overall NDSU research community. A breeding colony of nod-SCID- gamma (NSG™) mice was founded and used in multiple grafting studies, including subcutaneous, orthotopic, and patient-derived xenograft implantations. The Core also established a colony of genetically engineered LSL-KrasG12D/+; LSL-Trp53R172H/+; Pdx-1-Cre (KPC) mice to complement these NSG™ studies. These achievements allowed the Animal Core to complete our original goals of enhancing basic and translational studies devoted to detecting and treating pancreatic cancer and strengthening biomedical research at NDSU. For Phase II, the Animal Core aims are 1) to support the scientific and animal needs of individual Junior Investigators, Pilot Project Investigators, new and existing faculty, postdoctoral fellows, and graduate students associated with the CDTSPC; and 2) to increase the sustainability of the Animal Core Research platform. These aims will be achieved by establishing new colonies of genetically engineered models (including KC- smad4 and KC-Tgfbr2 strains), establishing a tissue biobank for archival maintenance of PDAC and other cancer samples, and expanding services to include on-site histology analysis and advanced imaging modalities.

项目总结/摘要（动物核心机构）动物模型在建立和改进各种疾病的治疗方法中起着至关重要的作用。癌症，包括胰腺导管腺癌（PDAC）。因此，当NDSU诊断中心和治疗策略，其中一个关键组成部分是动物研究核心设施。在该项目的第一阶段，动物核心做出了重大贡献， COBRE项目的使命和整个NDSU研究界。一个繁殖群， γ（NSG™）小鼠并用于多种移植研究，包括皮下，原位，和患者来源的异种移植物排斥。内核还建立了一个基因工程殖民地 LSL-KrasG 12 D/+; LSL-Trp 53 R172 H/+; Pdx-1-Cre（KPC）小鼠，以补充这些NSG™研究。这些这些成就使动物核心完成了我们最初的目标，即提高基础和翻译能力，致力于检测和治疗胰腺癌的研究，并加强在NDSU的生物医学研究。对于第二阶段，动物核心的目标是1）支持个别青少年的科学和动物需求研究人员，试点项目研究人员，新的和现有的教师，博士后研究员和研究生与CDTSPC相关;以及2）增加动物核心研究平台的可持续性。这些目标将通过建立新的基因工程模型（包括KC- smad 4和KC-Tgfbr 2菌株），建立用于PDAC和其他癌症样本，并扩大服务，包括现场组织学分析和先进的成像方式。